BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	DH1	2,204,804	Δ120 bp	intergenic (‑239/‑54)	tqsA ← / → mdtJ	AI‑2 transporter TqsA/spermidine export protein MdtJ

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	DH1	2204804	2204923	120	270 [3]	[0] 266	tqsA/mdtJ	AI‑2 transporter TqsA/spermidine export protein MdtJ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	DH1	= 2204803	3 (0.010)	263 (0.530)	160/296	2.2	99.4%	intergenic (‑238/‑174)	tqsA/mdtJ	AI‑2 transporter TqsA/spermidine export protein MdtJ
*	?	DH1	2204924 =	0 (0.000)	263 (0.530)	160/296	2.2	99.4%	intergenic (‑359/‑53)	tqsA/mdtJ	AI‑2 transporter TqsA/spermidine export protein MdtJ

Only 100 of 266 total aligned reads displayed.

TGTTCAGGACGTTATTTTCTACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGG  >  DH1/2204779‑2205074

aatattttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTc                                                                                                                                                   <  2:1787680‑M2/6‑1 (MQ=255)

aatattttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGATTc                                                                                                                                                   >  8:1409231‑M2/146‑151 (MQ=255)

    ttttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTg                                                                                                                                               >  2:31970‑M2/142‑151 (MQ=255)

     tttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTg                                                                                                                                               <  5:824604‑M2/10‑1 (MQ=255)

     tttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTg                                                                                                                                               >  7:1725274‑M2/141‑150 (MQ=255)

     tttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTg                                                                                                                                               <  2:1187946‑M2/10‑1 (MQ=255)

      ttgagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacggtattttctacattGGTTTCGCTGa                                                                                                                                              >  1:380486‑M2/140‑150 (MQ=255)

        gagttaattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAAtt                                                                                                                                           <  7:368230‑M2/14‑1 (MQ=255)

             aattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatgcgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCg                                                                                                                                      <  7:893999‑M2/19‑1 (MQ=255)

             aattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGc                                                                                                                                       <  6:845613‑M2/18‑1 (MQ=255)

             aattcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCg                                                                                                                                      >  6:601193‑M2/133‑151 (MQ=255)

                tcttaagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGaa                                                                                                                                    >  1:1483923‑M2/130‑150 (MQ=255)

                   taagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGaaa                                                                                                                                   <  6:657003‑M2/22‑1 (MQ=255)

                   taagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGaaa                                                                                                                                   <  3:1286837‑M2/22‑1 (MQ=255)

                   taagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAAt                                                                                                                                 <  1:1085118‑M2/24‑1 (MQ=255)

                    aagctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAAtt                                                                                                                                <  2:1335183‑M2/25‑1 (MQ=255)

                     agctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTa                                                                                                                               >  6:306227‑M2/125‑150 (MQ=255)

                       ctatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTaaa                                                                                                                             >  3:663669‑M2/123‑150 (MQ=255)

                        tatgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAaat                                                                                                                           >  5:1283214‑M2/122‑151 (MQ=255)

                         atgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTaaaa                                                                                                                            <  2:1512117‑M2/29‑1 (MQ=255)

                         atgatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAaat                                                                                                                           <  6:1476856‑M2/30‑1 (MQ=255)

                           gatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAaataat                                                                                                                        <  4:921304‑M2/33‑1 (MQ=255)

                           gatacaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAaataa                                                                                                                         <  1:484366‑M2/32‑1 (MQ=255)

                              acaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATtct                                                                                                                     >  3:411846‑M2/116‑151 (MQ=255)

                              acaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATtct                                                                                                                     >  4:1143798‑M2/116‑151 (MQ=255)

                              acaaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATtct                                                                                                                     <  2:337506‑M2/36‑1 (MQ=255)

                                aaatcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTctct                                                                                                                   >  3:1139667‑M2/114‑151 (MQ=255)

                                aaatcaggcgtgttcaactaccgaggacaattatcaaccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTctct                                                                                                                   <  7:459527‑M2/38‑1 (MQ=255)

                                   tcaggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGc                                                                                                                >  3:1908493‑M2/111‑151 (MQ=255)

                                    caggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGc                                                                                                                >  5:1169126‑M2/110‑150 (MQ=255)

                                    caggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGc                                                                                                                >  4:1434588‑M2/110‑150 (MQ=255)

                                    caggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCa                                                                                                               <  1:942366‑M2/42‑1 (MQ=255)

                                     aggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAg                                                                                                              <  5:1583220‑M2/43‑1 (MQ=255)

                                      ggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAgg                                                                                                             >  6:144017‑M2/108‑151 (MQ=255)

                                      ggcgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAgg                                                                                                             <  1:1210223‑M2/44‑1 (MQ=255)

                                        cgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAgg                                                                                                             >  8:355001‑M2/106‑149 (MQ=255)

                                        cgtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGgag                                                                                                           <  5:76038‑M2/46‑1 (MQ=255)

                                         gtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAgg                                                                                                             >  2:1474529‑M2/105‑148 (MQ=255)

                                         gtgttcaactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGgaga                                                                                                          <  1:634558‑M2/47‑1 (MQ=255)

                                               aactaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGAc                                                                                                     >  6:410392‑M2/99‑150 (MQ=255)

                                                 ctaccaaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGAGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAAt                                                                                                  >  5:374048‑M2/97‑151 (MQ=255)

                                                  aaccgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATg                                                                                                 <  3:1066724‑M2/56‑1 (MQ=255)

                                                   accgaggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATg                                                                                                 <  4:1908239‑M2/56‑1 (MQ=255)

                                                        ggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtatt                                                                                           >  1:703018‑M2/90‑151 (MQ=255)

                                                        ggacaattatcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtatt                                                                                           <  7:1320593‑M2/62‑1 (MQ=255)

                                                         gacaattatcatccgcgatgacgagaagctacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtattt                                                                                          >  2:399219‑M2/89‑151 (MQ=255)

                                                                atcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGa                                                                                    <  7:287882‑M2/69‑1 (MQ=255)

                                                                atcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAt                                                                                   >  5:1527886‑M2/82‑151 (MQ=255)

                                                                 tcatccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAtt                                                                                  >  4:496422‑M2/81‑151 (MQ=255)

                                                                     ccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTtta                                                                               <  4:1136002‑M2/74‑1 (MQ=255)

                                                                     ccgcgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTtta                                                                               <  2:797725‑M2/74‑1 (MQ=255)

                                                                       gcgatcacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTttatta                                                                            <  7:185337‑M2/77‑1 (MQ=255)

                                                                        cgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTttatt                                                                             >  5:1904378‑M2/74‑149 (MQ=255)

                                                                        cgatgacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAg                                                                           <  3:1663500‑M2/78‑1 (MQ=255)

                                                                            gacgagaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTc                                                                        <  6:1945573‑M2/81‑1 (MQ=255)

                                                                                agaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCt                                                                   >  8:1207610‑M2/66‑151 (MQ=255)

                                                                                 gaagcaacactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTa                                                                  <  1:116215‑M2/87‑1 (MQ=255)

                                                                                       acactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCt                                                             >  2:622081‑M2/59‑150 (MQ=255)

                                                                                        cactgcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTAc                                                           >  6:1295467‑M2/58‑151 (MQ=255)

                                                                                            gcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAg                                                         <  2:1703144‑M2/96‑1 (MQ=255)

                                                                                            gcggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAg                                                         <  8:1810633‑M2/96‑1 (MQ=255)

                                                                                             cggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGaaa                                                      <  6:1634992‑M2/99‑1 (MQ=255)

                                                                                             cggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGaa                                                       >  3:886633‑M2/53‑150 (MQ=255)

                                                                                              ggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAAt                                                     <  1:253805‑M2/100‑1 (MQ=255)

                                                                                              ggataattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAAt                                                     <  5:1461270‑M2/100‑1 (MQ=255)

                                                                                                 taattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAc                                                  >  3:1612233‑M2/49‑151 (MQ=255)

                                                                                                 taattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAc                                                  >  5:164301‑M2/49‑151 (MQ=255)

                                                                                                  aattgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATtat                                                  <  5:411240‑M2/103‑2 (MQ=255)

                                                                                                     tgtaatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCgg                                               <  8:266588‑M2/106‑1 (MQ=255)

                                                                                                       taatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTAc                                            >  3:594837‑M2/43‑151 (MQ=255)

                                                                                                       taatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTA‑TAGGTCTGGCTATTGCTACAGAAATTACCGGTAc                                            <  7:1234740‑M2/108‑1 (MQ=255)

                                                                                                        aatattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACg                                           >  2:479208‑M2/42‑151 (MQ=255)

                                                                                                        aatattatggacaatttgtgcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACg                                           >  5:1353914‑M2/42‑151 (MQ=255)

                                                                                                         atattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGc                                          <  8:1463129‑M2/111‑1 (MQ=255)

                                                                                                          tattatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCt                                         >  3:714786‑M2/40‑151 (MQ=255)

                                                                                                           attatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTg                                        >  3:332429‑M2/39‑151 (MQ=255)

                                                                                                           attatggacaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTg                                        >  1:1624244‑M2/39‑151 (MQ=255)

                                                                                                                  acaatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGa                                 <  3:833856‑M2/120‑1 (MQ=255)

                                                                                                                    aatttgttcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGaa                                >  4:1729832‑M2/30‑150 (MQ=255)

                                                                                                                           tcaggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCg                         >  7:1582358‑M2/23‑150 (MQ=255)

                                                                                                                            caggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCg                         >  3:973671‑M2/22‑149 (MQ=255)

                                                                                                                             aggacgttattttctacattGGTTTCTCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGc                      <  4:728755‑M2/131‑1 (MQ=255)

                                                                                                                             aggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGAGAATTTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGagg                      <  4:102075‑M2/131‑2 (MQ=255)

                                                                                                                             aggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGc                      <  1:1673336‑M2/131‑1 (MQ=255)

                                                                                                                             aggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGc                      >  7:104159‑M2/21‑151 (MQ=255)

                                                                                                                              ggacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCg                     >  8:1553481‑M2/20‑151 (MQ=255)

                                                                                                                               gacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATCAAATGGGCGAGCGt                    >  4:402274‑M2/19‑151 (MQ=255)

                                                                                                                               cacgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACACAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGt                    >  8:812622‑M2/19‑151 (MQ=255)

                                                                                                                                acgttattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTc                   >  1:87259‑M2/18‑151 (MQ=255)

                                                                                                                                    tattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTg               >  2:1416797‑M2/14‑151 (MQ=255)

                                                                                                                                    tattttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTg               >  5:583155‑M2/14‑151 (MQ=255)

                                                                                                                                       tttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGAGAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAg             <  4:949483‑M2/140‑1 (MQ=255)

                                                                                                                                       tttctacattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAg             <  8:539927‑M2/140‑1 (MQ=255)

                                                                                                                                            acattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGaa         <  5:1918947‑M2/144‑1 (MQ=255)

                                                                                                                                            acattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGaa         <  8:1830485‑M2/144‑1 (MQ=255)

                                                                                                                                            acattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAAt       >  1:1906165‑M2/6‑151 (MQ=255)

                                                                                                                                            acattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAAt       >  8:1299545‑M2/6‑151 (MQ=255)

                                                                                                                                             cattGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATg      <  1:1027273‑M2/147‑1 (MQ=255)

                                                                                                                                                tGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGc    <  5:1729483‑M2/149‑1 (MQ=255)

                                                                                                                                                 ggTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATggcgg  <  2:1607791/151‑1 (MQ=255)

TGTTCAGGACGTTATTTTCTACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGG  >  DH1/2204779‑2205074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`