New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3894523	34 (0.880)	4 (0.110)	4/294	NT	10.1%	coding (1058/1617 nt)	bglH	carbohydrate‑specific outer membrane porin, cryptic
	?	CP009273	= 3894651	39 (1.080)					coding (930/1617 nt)	bglH	carbohydrate‑specific outer membrane porin, cryptic

AATAGATTCGAAATCAGAGTGGGCGCCTGTTTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCG  >  CP009273/3894261‑3894750                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    aaTAGATTCGAAATCAGATTGGGCTCCTGTTTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTc                                                                                                                                                                                                                          <  1:320662/274‑1 (MQ=255)                        cgcCTGATTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGTCAATATAGGCTTCGCCCTGCGAATCCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTCAAGGTGGTAAATGGACTTGCGCAAGCATAACGGCCAAAGTTACTGGCGATAGCGTAATTCGCGACCAGGAAGGAGAAATCGTTAAAGCCACCTTAATCAAATTTCTGCGTTAAAGGTGTGACAAGCaca                                                                                                                                                                                                  >  2:5978/1‑273 (MQ=255)                                       nntatagagCGGTACCGAAGGAGTAAACCATCGCGTTAGATACAATGAAATGATCGCCAATATAGGCTTCGCACTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTcc                                                                                                                                                                                     <  2:307952/271‑1 (MQ=255)                                                cGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTCGAAGCCACCTTTATCAAATTTCTGCTTTAAAGATGTTACAAACATCCAGGTCTCTTTCCCGTCataata                                                                                                                                                                           >  2:680749/1‑274 (MQ=255)                                                cGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGCACTTGCGCCACCCTAACGGCCANAGTTACTGGCGATAGAGTTATTCGCGACCCGGAAGGAGAATTCGTTGAAGCCACCTTTATCCACTTTCTGCGTTCAAGATGTGCCAAACATCCAGATATCTTTCCAGTCATAATAc                                                                                                                                                                          >  1:668253/1‑275 (MQ=255)                                                   tACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATAccc                                                                                                                                                                        >  1:415370/1‑274 (MQ=255)                                                                          ttAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCaa                                                                                                                                                                                                                               >  2:249799/1‑196 (MQ=255)                                                                              cTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAAttt                                                                                                                                                                                                                           <  1:249804/196‑1 (MQ=255)                                                                                                  agataGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATAACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGAc                                                                                                                        <  1:680758/273‑1 (MQ=255)                                                                                                          ctcccccTGCTAATTCAGACGGACCGGTCTTCCGCGTGTGTGATCACCATTATTACGACCATTGGAGGTGGAAGATGGGCGTGCGCCAGAATAACGGATAAAGTTACTGGCGTGAGAGGTAGTCGCGAGCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAAGTTCTGCGTTGAAGATTTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCGGATTAGCTTTTTCGGATGCGCTTTCGTTTGCCGTGACATAACGAc                                                                                                                <  2:668244/271‑1 (MQ=255)                                                                                                                                      gTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTAGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATg                                                                                                                                           >  2:165233/1‑220 (MQ=255)                                                                                                                                          cgccgGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTAGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCt                                                                                                                                       <  1:165236/220‑1 (MQ=255)                                                                                                                                           gccgGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGAGCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATaa                                                                                 <  1:399671/273‑1 (MQ=255)                                                                                                                                                          ataataACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATc                                                                                       >  1:263548/1‑252 (MQ=255)                                                                                                                                                              taaCGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCAt                                                                                   <  2:263543/252‑1 (MQ=255)                                                                                                                                                                   aCCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAg                                                                                                                                                                                               >  1:39979/1‑139 (MQ=255)                                                                                                                                                                   aCCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTc                                                                                                                                                                                      >  2:292189/1‑148 (MQ=255)                                                                                                                                                                   aCCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCTCTTTCGTTTGCCGTGCCATAACGAACACTTACCTTTCATTTGGCTTTATCCCAtatctttatatctttaatatttaaattaa                                                       >  2:364021/1‑262 (MQ=255)                                                                                                                                                                   aCCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATa                                                                                  >  2:106216/1‑248 (MQ=255)                                                                                                                                                                   aCCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATACCATAACGGGATATCTTTATAGCGtaaataaa                                                       >  1:149257/1‑275 (MQ=255)                                                                                                                                                                     cATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGNTACTGGCGNTAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCTGTGACATCACGACCACTTACCATTaaggtggctt                                                                                           >  1:465218‑M2/1‑227 (MQ=255)                                                                                                                                                                       ttAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTAt                                                                                                                                                                                           <  2:39979/139‑1 (MQ=255)                                                                                                                                                                       ttAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGt                                                                                                                                                                                  <  1:292195/148‑1 (MQ=255)                                                                                                                                                                       ttAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACgg                                                                              <  1:106217/248‑1 (MQ=255)                                                                                                                                                                            ggtggtAAATGGACTTGCGCCAGTATAAAAGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTaatggtaagtggtcgttatgtcacggcaaccgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagataccnggatgtttggcccatctttaac                                                                                                                   >  1:508864‑M1/1‑92 (MQ=255)                                                                                                                                                                                ggaaatgGACTTGCGGCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGGGAATTCGTTGAAGCCACCTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagagacctggatgtttggcacatctttaacgcag                                                                                                               <  2:508857‑M1/204‑119 (MQ=255)                                                                                                                                                                                       gACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCataa                                                                                                                                                                             >  2:398521/1‑137 (MQ=255)                                                                                                                                                                                          ttGCGCCAGCATAACGGCCTAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAAc                                                                                >  1:568626/1‑227 (MQ=255)                                                                                                                                                                                          ttGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagatacctg                                                                                                                                        >  1:40966‑M1/1‑78 (MQ=255)                                                                                                                                                                                           tGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATAcc                                                                                                                                                                         <  1:398528/137‑1 (MQ=255)                                                                                                                                                                                              gcCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagatacctggatg                                                                                                                                    <  2:40966‑M1/171‑98 (MQ=255)                                                                                                                                                                                              gcCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGa                                                                            <  2:568617/227‑1 (MQ=255)                                                                                                                                                                                                                                  aTTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCaaa                                                                                                                                                                                                                              >  1:288154/1‑45 (MQ=255)                                                                                                                                                                                                                                      gcgACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTc                                                                                                                                                                                                                          <  2:288149/45‑1 (MQ=255)                                                                                                                                                                                                                                                 ggaGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAgg                                                                                                                                                                                              <  1:159940/62‑1 (MQ=255)                                                                                                                                                                                                                                                       ttCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATc                                                                        >  1:694014/1‑174 (MQ=255)                                                                                                                                                                                                                                                           ttGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTta                                                                    <  2:694005/174‑1 (MQ=255)                                                                                                                                                                                                                                                            tGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACa                                                                                                                       >  1:184162/1‑122 (MQ=255)                                                                                                                                                                                                                                                              aaGCCACCTTaatggtaagtggtcgtgatgtcacagcaaacgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagatacctggatgtttggcacatctttaacgcagaaatttgataaaggtggcttcaacgaattctccttcctggtcgcgaataactctancgccagtanctttggccgttatgctggcgcaagtccatttaccacctttaatg  >  1:465218‑M1/1‑10 (MQ=255)                                                                                                                                                                                                                                                                gCCACCTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcggaaaaagataatcaggataataacgggtattatgactggaaagatacctggatgtttgg                                                                                                                               <  2:181862‑M1/110‑103 (MQ=255)                                                                                                                                                                                                                                                                gCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTGGCCGTGACATAAc                                                                                                                   <  2:184159/122‑1 (MQ=255)                                                                                                                                                                                                                                                                    ccTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcggaaaaaga                                                                                                                                                                                  >  1:629698‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                                                    ccTTaatggtaagtggtcgttatgtcacggcaaacgaaagcgcatcgg                                                                                                                                                                                         >  1:86797‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                                                    ccTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACCACCACTTACCATTAAGGTGGCTTTATCCCATAAc                                                                                >  1:311085/1‑153 (MQ=255)                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    AATAGATTCGAAATCAGAGTGGGCGCCTGTTTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCG  >  CP009273/3894261‑3894750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.