breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT45.6% 30.9 / 46.0 57S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,363,1510TC44.3% 70.3 / 242.4 192G115G (GGT→GGCyhcDputative fimbrial usher protein YhcD
*NC_0009133,556,2070TC36.4% 63.2 / 14.8 44S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009132,001,9180AC34.2% 55.6 / 14.0 38intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009133,556,2050AC31.8% 72.5 / 11.1 44S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,825,0610GA30.4% 70.7 / 17.2 46intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009134,568,5040TG29.1% 215.7 / 14.9 111T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_00091329,4960TA28.3% 99.0 / 18.2 46intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009134,101,5140GT28.3% 106.5 / 15.9 46intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,111,7470GT28.2% 94.4 / 12.2 39N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_000913445,5580AC28.1% 51.5 / 14.4 32V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
*NC_0009133,404,2180TG27.8% 105.7 / 13.8 54G245G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_0009133,404,2150TG27.1% 115.9 / 14.3 59G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_000913650,8090TG25.9% 121.6 / 12.2 58T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009132,326,4500TG25.8% 126.6 / 27.7 66G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_000913372,6400TG25.6% 171.9 / 20.3 82L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009134,032,7310TG25.4% 114.7 / 12.7 59G80G (GGT→GGGyigZIMPACT family member YigZ
*NC_000913753,1250TA25.0% 96.6 / 11.1 40intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_000913332,9910GT24.4% 207.3 / 27.0 82L207F (TTG→TTTpdeLDNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
*NC_0009134,287,7190TA24.2% 156.2 / 19.8 66intergenic (‑348/‑45)acs/nrfAacetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 608007 =NA (NA)6 (0.080) 4/224 7.1 5.8% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 97 (1.330)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 374945 =NA (NA)4 (0.050)
+TACGCCGCATCCGGT
4/246 7.5 NA noncoding (15/34 nt) other REP31a
?NC_000913 377422 = NA (NA)noncoding (1/34 nt) other REP32b
* ? NC_000913 = 297353789 (0.990)3 (0.030) 3/270 8.6 3.4% coding (326/1194 nt) lplT lysophospholipid transporter
?NC_000913 2973541 = 84 (0.950)coding (322/1194 nt) lplT lysophospholipid transporter
* ? NC_000913 = 217336264 (0.710)7 (0.080) 3/272 8.6 9.4% pseudogene (917/1358 nt) gatC galactitol‑specific PTS enzyme IIC component
?NC_000913 2173368 = 72 (0.810)pseudogene (911/1358 nt) gatC galactitol‑specific PTS enzyme IIC component
* ? NC_000913 339772 =NA (NA)3 (0.030)
+T
3/274 8.6 NA noncoding (15/34 nt) other REP23a
?NC_000913 377408 = NA (NA)noncoding (15/34 nt) other REP32b
* ? NC_000913 511043 =76 (0.840)3 (0.030) 3/276 8.7 3.8% coding (337/2505 nt) copA Cu(+) exporting P‑type ATPase
?NC_000913 511081 = 78 (0.860)coding (299/2505 nt) copA Cu(+) exporting P‑type ATPase