breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 644,464 C→A E180D (GAG→GAT rna ← RNase I
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 961,862 G→T R221L (CGC→CTC)  cmk → cytidylate kinase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,333,844 G→T intergenic (+199/‑11) topA → / → cysB DNA topoisomerase 1/DNA‑binding transcriptional dual regulator CysB
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,843,297 G→T Q309H (CAG→CAT gdhA → glutamate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,890,026 G→T G169G (GGC→GGA yeaY ← Slp family lipoprotein YeaY
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,981,085 C→A Q176H (CAG→CAT otsA ← trehalose‑6‑phosphate synthase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,360,363 G→T P351Q (CCG→CAG)  rhmD ← L‑rhamnonate dehydratase
RA 3,243,503 G→T S413R (AGC→AGA uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,435,412 C→A P69Q (CCG→CAG)  rsmB → 16S rRNA m(5)C967 methyltransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,430,255 C→A P131H (CCC→CAC)  cycA → serine/alanine/glycine/cycloserine:H(+)symporter
RA 4,472,227 G→T R57S (CGC→AGC)  pyrB ← aspartate carbamoyltransferase catalytic subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211436 15162 34 [30] [28] 33 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102960 3104073 1114 31 [30] [30] 31 mutY mutY
* * ÷ NC_000913 3423770–3424234 3424533–3424239 6–764 32 [26] [30] 33 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)173 (1.510) 89/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =205 (1.740)219 (1.870) 83/274 0.1 67.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)164 (1.390) 79/276 0.1 96.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA