breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 308,274 G→T S353R (AGC→AGA ecpC ← putative fimbrial usher protein EcpC
RA 599,560 G→T E283* (GAA→TAA)  cusA → copper/silver export system RND permease
RA 700,386 C→A L403F (TTG→TTT nagC ← DNA‑binding transcriptional dual regulator NagC
RA 779,896 G→T S100I (AGC→ATC)  cpoB → cell division coordinator CpoB
RA 867,867 G→T S116S (TCG→TCT gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 973,599 C→A T21T (ACC→ACA cmoM → tRNA cmo(5)U34 methyltransferase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,184,456 G→T Q21K (CAG→AAG)  thiD ← bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,587,305 G→T P121P (CCG→CCT yhhA → DUF2756 domain‑containing protein YhhA
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,987,883 G→T intergenic (‑1/+2) hemY ← / ← hemX protein HemY/PF04375 family protein HemX
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,347,718 C→A A343S (GCC→TCC)  dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,360,559 A→C V459G (GTT→GGT)  cadC ← DNA‑binding transcriptional activator CadC
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196255 1211443 15189 35 [30] [33] 35 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102986 3104073 1088 35 [30] [34] 36 mutY mutY
* * ÷ NC_000913 3423768–3424234 3424534–3424239 6–767 36 [32] [33] 35 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)93 (1.190) 20/190 2.4 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)89 (1.140) 29/190 1.2 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375102 =NA (NA)45 (0.510) 24/216 2.3 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375203 =NA (NA)81 (0.910) 32/216 1.4 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 12994980 (0.000)107 (0.970) 69/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =180 (1.580)166 (1.470) 79/274 0.1 64.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)123 (1.080) 66/276 0.3 96.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA