breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,604,344 | 0 | C | T | 39.6% | 103.5 / 60.3 | 96 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 4,111,747 | 0 | G | T | 31.4% | 74.3 / 18.6 | 35 | N156K (AAC→AAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 2,326,450 | 0 | T | G | 31.3% | 95.4 / 43.0 | 67 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 3,206,435 | 0 | C | T | 29.5% | 95.0 / 32.7 | 61 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 2,001,918 | 0 | A | C | 28.3% | 106.6 / 23.5 | 60 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
* | NC_000913 | 3,608,719 | 0 | G | A | 28.0% | 84.8 / 12.9 | 50 | intergenic (+70/+32) | zntA/tusA | Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase/sulfur transfer protein TusA |
* | NC_000913 | 4,101,507 | 0 | T | G | 27.2% | 165.9 / 14.1 | 81 | intergenic (+77/‑183) | sodA/kdgT | superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter |
* | NC_000913 | 3,556,205 | 0 | A | C | 26.8% | 113.1 / 10.8 | 56 | S215R (AGT→AGG) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 1,645,034 | 0 | A | C | 26.7% | 113.5 / 10.7 | 60 | intergenic (‑132/+85) | rem/hokD | Qin prophage; protein Rem/Qin prophage; toxic protein HokD |
* | NC_000913 | 4,628,649 | 0 | A | C | 26.7% | 113.2 / 16.5 | 60 | intergenic (+102/+206) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
* | NC_000913 | 1,825,061 | 0 | G | A | 26.1% | 88.2 / 14.0 | 46 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
* | NC_000913 | 372,640 | 0 | T | G | 25.9% | 114.6 / 14.5 | 58 | L176V (TTA→GTA) | mhpD | 2‑hydroxypentadienoate hydratase |
* | NC_000913 | 888,014 | 0 | T | G | 25.8% | 130.8 / 12.6 | 66 | noncoding (44/81 nt) | rybB | small regulatory RNA RybB |
* | NC_000913 | 3,404,215 | 0 | T | G | 25.3% | 158.7 / 32.9 | 83 | G244G (GGT→GGG) | yhdH | acrylyl‑CoA reductase |
* | NC_000913 | 29,496 | 0 | T | A | 25.0% | 160.9 / 31.3 | 72 | intergenic (+301/‑155) | dapB/carA | 4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha |
* | NC_000913 | 3,206,444 | 0 | T | A | 24.6% | 125.5 / 14.0 | 61 | intergenic (‑188/‑19) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 646,747 | 0 | T | G | 24.5% | 96.5 / 23.3 | 53 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
* | NC_000913 | 770,897 | 0 | T | G | 23.9% | 131.7 / 10.3 | 71 | intergenic (+286/‑561) | mngB/cydA | alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I |
* | NC_000913 | 753,125 | 0 | T | A | 23.9% | 151.5 / 23.0 | 67 | intergenic (‑330/+60) | ybgD/gltA | putative fimbrial protein YbgD/citrate synthase |
* | NC_000913 | 445,558 | 0 | A | C | 23.7% | 69.0 / 13.8 | 38 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
Marginal new junction evidence (lowest skew 10 of 32 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 374921 = | NA (NA) | 60 (0.880) | 14/190 | 3.1 | NA | intergenic (+40/‑538) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377531 = | NA (NA) | intergenic (+220/+4) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 374922 = | NA (NA) | 72 (0.770) | 18/260 | 3.6 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377329 = | NA (NA) | intergenic (+18/+206) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375023 = | NA (NA) | 52 (0.760) | 9/192 | 4.4 | NA | intergenic (+142/‑436) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | NA (NA) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 375160 | NA (NA) | 41 (0.420) +T |
14/274 | 4.6 | NA | noncoding (28/34 nt) | other | REP31e |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | = 375219 | NA (NA) | 13 (0.170) | 9/216 | 5.0 | NA | noncoding (1/36 nt) | other | REP31f |
? | NC_000913 | = 377333 | NA (NA) | intergenic (+22/+202) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375248 = | NA (NA) | 54 (0.630) | 10/238 | 5.1 | NA | noncoding (15/34 nt) | other | REP31g |
? | NC_000913 | 377507 = | NA (NA) | noncoding (16/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | = 375064 | NA (NA) | 8 (0.120) | 7/192 | 5.1 | NA | noncoding (33/34 nt) | other | REP31c |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 340035 = | NA (NA) | 36 (0.370) | 11/274 | 5.4 | NA | coding (243/297 nt) | yahH | putative uncharacterized protein YahH |
? | NC_000913 | 377322 = | NA (NA) | intergenic (+11/+213) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 375246 | NA (NA) | 10 (0.110) +GAACGCCTTATCCGGT |
8/244 | 5.9 | NA | noncoding (13/34 nt) | other | REP31g |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | 377321 = | NA (NA) | 26 (0.270) | 9/268 | 6.0 | NA | intergenic (+10/+214) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |
? | NC_000913 | = 508817 | NA (NA) | noncoding (2/34 nt) | other | REP48e |