breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 430,451 C→A A316E (GCG→GAG)  secF → Sec translocon accessory complex subunit SecF
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,506,124 C→A intergenic (‑44/‑48) ydcO ← / → sutR putative transport protein YdcO/DNA‑binding transcriptional dual regulator SutR
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,138,486 C→A G13W (GGG→TGG)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase
RA 3,247,261 C→A R204S (CGC→AGC)  exuR → DNA‑binding transcriptional repressor ExuR
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,584,196 Δ743 bp IS1‑mediated yrhA[yrhB] yrhA, yrhD, [yrhB]
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102978 3104051 1074 26 [22] [25] 30 [mutY] [mutY]
* * ÷ NC_000913 3423765–3424234 3424525–3424238 5–761 27 [25] [22] 38 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)50 (0.760) 17/190 2.2 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)31 (0.470) 15/190 2.6 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375203 =NA (NA)44 (0.590) 26/216 1.5 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 1207790 =100 (1.040)38 (0.450) 28/244 1.6 30.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 87 (1.030)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)87 (0.930) 50/268 0.5 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =159 (1.660)175 (1.840) 81/274 0.0 68.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)103 (1.070) 68/276 0.1 95.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA