breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,604,344 | 0 | C | T | 37.2% | 129.4 / 63.6 | 102 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 4,568,504 | 0 | T | G | 32.5% | 234.6 / 24.6 | 127 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
* | NC_000913 | 4,111,747 | 0 | G | T | 32.4% | 154.3 / 43.2 | 71 | N156K (AAC→AAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 445,558 | 0 | A | C | 32.3% | 107.4 / 32.9 | 65 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
* | NC_000913 | 753,125 | 0 | T | A | 29.6% | 128.5 / 29.3 | 71 | intergenic (‑330/+60) | ybgD/gltA | putative fimbrial protein YbgD/citrate synthase |
* | NC_000913 | 3,206,435 | 0 | C | T | 29.4% | 114.8 / 33.4 | 68 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 3,556,207 | 0 | T | C | 28.8% | 92.9 / 17.1 | 52 | S215G (AGT→GGT) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 2,001,918 | 0 | A | C | 28.1% | 166.1 / 28.0 | 82 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
* | NC_000913 | 4,101,507 | 0 | T | G | 27.6% | 156.4 / 11.0 | 76 | intergenic (+77/‑183) | sodA/kdgT | superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter |
* | NC_000913 | 1,825,061 | 0 | G | A | 27.1% | 86.0 / 17.0 | 48 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
* | NC_000913 | 3,556,205 | 0 | A | C | 26.9% | 104.1 / 19.0 | 52 | S215R (AGT→AGG) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 2,608,244 | 0 | T | G | 26.0% | 184.1 / 13.3 | 100 | V443G (GTG→GGG) | hyfF | hydrogenase 4 component F |
* | NC_000913 | 1,550,663 | 0 | A | C | 25.3% | 172.3 / 24.0 | 79 | N68T (AAC→ACC) | fdnH | formate dehydrogenase N subunit beta |
* | NC_000913 | 2,483,268 | 0 | T | A | 23.8% | 240.2 / 31.8 | 109 | L24F (TTA→TTT) | emrK | tripartite efflux pump membrane fusion protein EmrK |
* | NC_000913 | 2,326,450 | 0 | T | G | 23.5% | 187.5 / 34.3 | 85 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 4,628,649 | 0 | A | C | 23.4% | 184.5 / 16.8 | 78 | intergenic (+102/+206) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
* | NC_000913 | 4,111,752 | 0 | T | C | 23.2% | 167.6 / 11.5 | 82 | K155E (AAA→GAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 95,568 | 0 | C | A | 23.0% | 314.8 / 69.5 | 113 | L307M (CTG→ATG) | murF | D‑alanyl‑D‑alanine‑adding enzyme |
* | NC_000913 | 888,014 | 0 | T | G | 22.4% | 151.1 / 12.4 | 67 | noncoding (44/81 nt) | rybB | small regulatory RNA RybB |
* | NC_000913 | 3,492,017 | 0 | T | G | 22.1% | 209.5 / 11.9 | 87 | N94T (AAC→ACC) | ppiA | peptidyl‑prolyl cis‑trans isomerase A |
Marginal new junction evidence (lowest skew 10 of 22 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 374922 = | NA (NA) | 24 (0.180) | 13/258 | 5.6 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377329 = | NA (NA) | intergenic (+18/+206) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 374921 = | NA (NA) | 18 (0.190) | 7/188 | 6.0 | NA | intergenic (+40/‑538) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377531 = | NA (NA) | intergenic (+220/+4) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 4542690 | 90 (0.650) | 9 (0.070) | 9/256 | 6.8 | 8.1% | intergenic (+57/‑425) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | = 4542986 | 120 (0.920) | intergenic (+353/‑129) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | 4542682 = | 84 (0.600) | 11 (0.080) | 9/256 | 6.8 | 10.0% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 120 (0.920) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | 375124 = | NA (NA) | 5 (0.050) | 5/190 | 7.0 | NA | intergenic (+243/‑335) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | NA (NA) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 374922 = | NA (NA) | 10 (0.100) | 5/200 | 7.2 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377430 = | NA (NA) | intergenic (+119/+105) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 608007 = | NA (NA) | 9 (0.080) | 6/222 | 7.3 | 5.1% | noncoding (1/1345 nt) | IS186 | repeat region |
? | NC_000913 | 609352 = | 169 (1.500) | intergenic (+175/+107) | insL‑2/entD | IS186/IS421 transposase/phosphopantetheinyl transferase EntD | |||||
* | ? | NC_000913 | = 375165 | NA (NA) | 5 (0.050) | 4/190 | 7.5 | NA | noncoding (33/34 nt) | other | REP31e |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375023 = | NA (NA) | 17 (0.180) | 4/190 | 7.5 | NA | intergenic (+142/‑436) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | NA (NA) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 375160 | NA (NA) | 10 (0.070) +T |
7/272 | 7.8 | NA | noncoding (28/34 nt) | other | REP31e |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d |