breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,589,124 C→A G510G (GGC→GGA acrD → multidrug efflux pump RND permease AcrD
RA 2,890,653 C→A A416S (GCC→TCC)  cysJ ← sulfite reductase, flavoprotein subunit
RA 3,256,645 C→A D3Y (GAT→TAT)  cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,450,062 C→A A58S (GCT→TCT)  rplV ← 50S ribosomal subunit protein L22
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,624,269 G→T A174E (GCA→GAA)  ytjB ← protein Smp

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196254 1211433 15180 45 [38] [38] 46 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102950 3104078 1129 42 [41] [41] 42 mutY mutY
* * ÷ NC_000913 3423775–3424234 3424526–3424238 5–752 42 [41] [39] 44 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)181 (1.230) 96/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)172 (1.200) 83/260 0.1 99.4% intergenic (‑305/+16) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
?NC_000913 1979279 = 2 (0.010)intergenic (‑64/‑474) insA‑5/uspC IS1 protein InsA/universal stress protein C
* ? NC_000913 3363001 =234 (1.540)279 (1.850) 101/274 0.0 70.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)185 (1.220) 91/276 0.1 95.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA