breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 21,699 G→T R98L (CGT→CTT)  ribF → bifunctional riboflavin kinase/FMN adenylyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 990,012 C→G W115C (TGG→TGC pncB ← nicotinate phosphoribosyltransferase
RA 1,036,395 G→T M14I (ATG→ATT hyaE → putative HyaA chaperone
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,637,726 (T)8→7 coding (57/174 nt) gnsB ← Qin prophage; protein GnsB
RA 1,649,898 Δ1 bp coding (105/192 nt) ydfD → Qin prophage; lysis protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,703,612 C→A A233S (GCT→TCT)  ydgJ ← putative oxidoreductase YdgJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,835,689 C→A A579A (GCG→GCT hypF ← carbamoyltransferase HypF
RA 3,047,046 C→A E666* (GAA→TAA)  gcvP ← glycine decarboxylase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,315,367 C→A V58F (GTT→TTT)  phnO ← aminoalkylphosphonate N‑acetyltransferase
RA 4,472,556 G→T intergenic (‑23/+258) pyrL ← / ← yjgH pyrBI operon leader peptide/RutC family protein YjgH

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196256 1211435 15180 32 [30] [30] 31 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102994 3104052 1059 31 [29] [28] 32 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424528–3424238 5–759 31 [27] [30] 31 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)130 (1.070) 79/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =208 (1.660)240 (1.930) 103/274 0.0 69.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)165 (1.320) 75/276 0.2 94.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA