Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,581,699	(19-bp)2→1	intergenic (+60/+17)	tktB → / ← ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2581699	2581717	19	31 [0]	[0] 31	tktB/ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2581698	NA (NA)	27 (0.430)	23/238	1.2	NA	noncoding (36/36 nt)	other	REP179b
	?	NC_000913	2581718 =	NA (NA)					intergenic (+79/+16)	tktB/ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

CGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTG  >  NC_000913/2581585‑2581850                                                                                                                                      |                                                                                                                                     tcccgttctttggctttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCg                                                                                                                                >  2:552753‑M2/134‑140 (MQ=255)            ggttttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGttt                                                                                                                     <  1:625651‑M2/18‑1 (MQ=255)                ttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACg                                                                                                                 >  2:667074‑M2/119‑140 (MQ=255)                         agaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttacccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCca                                                                                                        <  2:1097523‑M2/31‑1 (MQ=255)                             tattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAAc                                                                                                    <  1:535219‑M2/35‑1 (MQ=255)                              attgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACg                                                                                                   <  1:673302‑M2/36‑1 (MQ=255)                                   ggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGc                                                                                              >  1:644217‑M2/100‑140 (MQ=255)                                    gcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGcc                                                                                             >  2:552211‑M2/99‑140 (MQ=255)                                     caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  1:1104711‑M2/98‑140 (MQ=255)                                     caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  1:264940‑M2/98‑140 (MQ=255)                                     caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  2:928297‑M2/98‑140 (MQ=255)                                     caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  1:1209323‑M2/98‑140 (MQ=255)                                     aaaaagcgcatagggtcctgggagtgaggggtgcctggtggtgattgccggatgtggattgccggatggggcgcggacgcgtcttatccggcttacaTGGCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            <  2:398455‑M2/43‑1 (MQ=255)                                                     gctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGc                                                                            <  2:80894‑M2/59‑1 (MQ=255)                                                            gtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     >  2:614897‑M2/75‑140 (MQ=255)                                                            gtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     <  2:1209323‑M2/66‑1 (MQ=255)                                                                   gtgcctgatggtgattgcgggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGt                                                              <  2:16484‑M2/73‑1 (MQ=255)                                                                      cctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCTCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTggg                                                           >  1:1205142‑M2/65‑140 (MQ=255)                                                                         gatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTc                                                        >  2:702644‑M2/62‑140 (MQ=255)                                                                          atggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCt                                                       >  1:287239‑M2/61‑140 (MQ=255)                                                                           tggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTg                                                      <  1:614897‑M2/81‑1 (MQ=255)                                                                             gtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTTTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcg                                                    <  2:451827‑M2/83‑1 (MQ=255)                                                                             gtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcg                                                    <  1:79094‑M2/83‑1 (MQ=255)                                                                                                      atgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCt                           >  1:940212‑M2/33‑140 (MQ=255)                                                                                                        gcgacgctgacgcgtcttatccggcctacaTGTCCCTCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGa                         <  2:1205142‑M2/110‑1 (MQ=255)                                                                                                           acgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCg                      >  2:383321‑M2/28‑140 (MQ=255)                                                                                                                 acgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAg                >  1:185521‑M2/22‑140 (MQ=255)                                                                                                                             cggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGcc    >  2:679378‑M2/10‑140 (MQ=255)                                                                                                                               gcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg  >  2:1180106‑M2/8‑140 (MQ=255)                                                                                                                               gcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg  >  2:568033‑M2/8‑140 (MQ=255)                                                                                                                               gcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg  <  1:667074‑M2/133‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTG  >  NC_000913/2581585‑2581850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑