breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT39.0% 113.8 / 64.4 100S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009132,001,9180AC36.7% 121.6 / 44.5 79intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_00091329,4960TA33.3% 108.8 / 34.8 66intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_000913501,5750AC31.5% 96.6 / 25.9 54V555G (GTG→GGG) ybaLputative transporter YbaL
*NC_000913753,1250TA31.4% 152.5 / 39.4 86intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_000913372,6400TG31.1% 136.2 / 24.1 74L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009133,556,2050AC29.8% 97.4 / 14.0 47S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,111,7470GT29.4% 118.0 / 26.7 51N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,111,7520TC28.6% 104.8 / 11.0 56K155E (AAA→GAA) yiiQDUF1454 domain‑containing protein YiiQ
*NC_000913501,5640AC28.0% 96.8 / 26.2 50*559E (TAA→GAA) ybaLputative transporter YbaL
*NC_0009133,556,2070TC27.7% 88.6 / 12.3 47S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,825,0610GA26.8% 104.9 / 17.8 56intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_000913501,5720AC26.4% 115.7 / 13.5 53V556G (GTG→GGG) ybaLputative transporter YbaL
*NC_0009131,491,0980AC25.6% 195.4 / 19.3 90Y66S (TAC→TCC) cybBcytochrome b561
*NC_0009131,491,1030AC24.2% 204.3 / 18.9 91T68P (ACC→CCC) cybBcytochrome b561
*NC_0009134,296,0600CT24.0% 248.9 / 73.4 125intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913753,1210CA23.6% 229.0 / 15.6 90intergenic (‑326/+64)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_000913753,1140GT22.9% 254.4 / 16.3 96intergenic (‑319/+71)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_0009131,335,6910TG22.7% 217.7 / 17.5 97intergenic (+24/‑140)ymiC/acnAprotein YmiC/aconitate hydratase 1
*NC_000913175,6940TG21.3% 214.0 / 18.7 89G196G (GGT→GGGclcAchloride:H(+) antiporter ClcA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1462089 =100 (0.980)6 (0.060) 5/252 8.3 5.2% coding (1197/1206 nt) paaJ beta‑ketoadipyl‑CoA thiolase
?NC_000913 1462223 = 128 (1.370)coding (99/1314 nt) paaK phenylacetate‑CoA ligase
* ? NC_000913 2728258 =NA (NA)4 (0.040) 4/262 9.1 NA noncoding (927/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 2728336 = NA (NA)noncoding (849/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 = 22668536 (0.350)4 (0.040) 4/262 9.1 10.5% noncoding (927/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 2728336 = NA (NA)noncoding (849/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 = 3410771121 (1.190)4 (0.040) 4/268 9.2 3.4% coding (492/966 nt) dusB tRNA‑dihydrouridine synthase B
?NC_000913 = 3410820 109 (1.100)coding (541/966 nt) dusB tRNA‑dihydrouridine synthase B
* ? NC_000913 250706 =96 (0.940)3 (0.030) 3/254 9.6 2.7% pseudogene (635/756 nt) lafU flagellar system protein, promoterless fragment
?NC_000913 250755 = 125 (1.330)pseudogene (684/756 nt) lafU flagellar system protein, promoterless fragment
* ? NC_000913 493785 =88 (0.860)3 (0.030) 3/262 9.7 3.3% coding (1694/1932 nt) dnaX DNA polymerase III subunit tau
?NC_000913 493879 = 93 (0.960)coding (1788/1932 nt) dnaX DNA polymerase III subunit tau
* ? NC_000913 2422045 =76 (0.750)3 (0.030) 3/266 9.8 3.6% coding (338/894 nt) yfcH putative NAD‑dependent epimerase YfcH
?NC_000913 2422157 = 88 (0.900)coding (450/894 nt) yfcH putative NAD‑dependent epimerase YfcH
* ? NC_000913 467996 =90 (0.880)3 (0.030) 3/264 9.8 2.8% coding (585/819 nt) cof HMP‑PP phosphatase
?NC_000913 468078 = 123 (1.260)coding (667/819 nt) cof HMP‑PP phosphatase
* ? NC_000913 = 2833944104 (1.020)3 (0.030) 3/276 10.0 3.0% coding (33/1134 nt) norW NADH:flavorubredoxin reductase
?NC_000913 2834305 = 93 (0.910)coding (394/1134 nt) norW NADH:flavorubredoxin reductase
* ? NC_000913 2393580 =108 (1.060)3 (0.030) 3/272 10.0 2.9% coding (1467/1842 nt) nuoL NADH:quinone oxidoreductase subunit L
?NC_000913 2393629 = 96 (0.960)coding (1418/1842 nt) nuoL NADH:quinone oxidoreductase subunit L