breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 15,253 +GC coding (1086/1131 nt) dnaJ → chaperone protein DnaJ
JC 254,298 (TCAGTCAGCAGT)1→2 coding (1419/1458 nt) pepD ← peptidase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 344,004 G→A intergenic (+71/‑172) yahK → / → yahL aldehyde reductase, NADPH‑dependent/uncharacterized protein YahL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,233,268 C→G V484L (GTC→CTC)  nhaB ← Na(+):H(+) antiporter NhaB
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC 2,337,701 (CTT)3→2 coding (1718‑1720/2628 nt) gyrA ← DNA gyrase subunit A
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
MC JC 3,370,930 Δ14 bp coding (94‑107/690 nt) nanE ← putative N‑acetylmannosamine‑6‑phosphate epimerase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,743,353 A→T K207N (AAA→AAT yiaK → 2,3‑diketo‑L‑gulonate reductase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696470 696576 107 23 [22] [22] 23 [glnX]–[glnV] [glnX],[glnV]
* * ÷ NC_000913 1196255 1211436 15182 25 [22] [22] 24 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1287301 1287607 307 24 [21] [21] 25 [tyrV]–[tyrT] [tyrV],[tyrT]
* * ÷ NC_000913 3423750–3424234 3424533–3424240 7–784 24 [21] [22] 23 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)76 (0.950) 46/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =170 (2.060)145 (1.770) 67/274 0.1 62.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)89 (1.080) 53/276 0.3 95.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA