breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA BN000925 9,791 A→G D797G (GAC→GGC)  tnpA → Tn1 transposase
RA BN000925 9,802 G→A D801N (GAC→AAC)  tnpA → Tn1 transposase
RA BN000925 10,089 A→C A896A (GCA→GCC tnpA → Tn1 transposase
RA BN000925 10,359 T→A G986G (GGT→GGA tnpA → Tn1 transposase
RA BN000925 12,239 +A intergenic (‑601/+1055) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,406 T→G intergenic (‑768/+888) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,688 T→A intergenic (‑1050/+606) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 14,765 A→C D239A (GAT→GCT)  tetA → tetracycline exporter protein
RA BN000925 14,957 C→G A303G (GCG→GGG)  tetA → tetracycline exporter protein
RA BN000925 15,561 Δ1 bp intergenic (+312/+320) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,639 C→G intergenic (+390/+242) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,898 +C coding (649/666 nt) upf16.5 ← hypothetical protein
RA BN000925 16,410 +T coding (137/666 nt) upf16.5 ← hypothetical protein
RA BN000925 31,028 C→G P35A (CCC→GCC)  trbP → TrbP protein
RA BN000925 36,655 A→G R116G (AGA→GGA) 
R108G (AGA→GGA) 
istA →
istA →
IS21 transposase
IS21 transposase
RA BN000925 38,388 A→C pseudogene (45/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,390 1 bp→AG pseudogene (47/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,393 C→T pseudogene (50/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,421 Δ1 bp pseudogene (78/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,510 Δ1 bp pseudogene (167/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,526 Δ1 bp intergenic (+12/‑232) upf35.8 → / → aphA probable truncation of theoretical product due to sequence errors/aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,136 Δ1 bp coding (379/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,147 Δ1 bp coding (390/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,179 +CG coding (422/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 53,069 +T intergenic (+42/+10) traM → / ← kfrC TraM protein/KfrC protein
RA BN000925 54,833 Δ1 bp intergenic (‑32/+127) kfrB ← / ← kfrA KfrB protein/KfrA coiled‑coil protein
RA BN000925 56,817 Δ1 bp coding (297/522 nt) korF ← KorF repressor protein
MC JC NC_000913 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA NC_000913 482,975 C→A A477S (GCA→TCA)  acrB ← multidrug efflux pump RND permease AcrB
JC JC NC_000913 486,094 IS2 (–) +5 bp coding (334‑338/648 nt) acrR → DNA‑binding transcriptional repressor AcrR
JC JC NC_000913 986,937 IS5 (–) +4 bp coding (43‑46/1089 nt) ompF ← outer membrane porin F
JC JC NC_000913 1,125,778 IS1 (–) +9 bp coding (217‑225/585 nt) rimJ → ribosomal‑protein‑S5‑alanine N‑acetyltransferase
RA NC_000913 1,258,818 G→T G10C (GGT→TGT)  ychH → stress‑induced protein
RA NC_000913 1,296,375 A→G F583L (TTC→CTC)  adhE ← aldehyde‑alcohol dehydrogenase
RA NC_000913 1,298,371 C→A intergenic (‑250/‑227) adhE ← / → ychE aldehyde‑alcohol dehydrogenase/putative inner membrane protein
MC JC NC_000913 1,299,499 Δ1,199 bp insH21 insH21
JC JC NC_000913 1,978,377 IS5 (+) +4 bp intergenic (‑180/+138) flhD ← / ← insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
RA NC_000913 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA NC_000913 2,249,587 A→T F11Y (TTT→TAT)  yeiE ← putative LysR‑type transcriptional regulator YeiE
MC JC NC_000913 3,366,751 Δ295 bp IS5‑mediated yhcE[yhcF] yhcE, [yhcF]
RA NC_000913 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA NC_000913 3,717,751 C→T L148L (CTG→TTG)  ghrB → glyoxylate reductase
RA NC_000913 4,212,973 Δ1 bp intergenic (+27/‑67) rrlE → / → rrfE 23S ribosomal RNA/5S ribosomal RNA
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC NC_000913 4,543,608 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (494‑499/549 nt) fimA → type 1 fimbriae major subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423870–3424234 3424449–3424239 6–580 24 [17] [17] 18 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 8073360 (0.000)51 (0.780)
+G
26/424 1.5 62.3% intergenic (‑55/+97) ybhC/ybhB outer membrane lipoprotein YhbC/putative kinase inhibitor
?NC_000913 = 4171849 62 (0.940)intergenic (+93/‑208) rrfB/murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
* ? NC_000913 1207790 =60 (0.910)25 (0.410) 24/394 1.5 33.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 45 (0.740)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780560 (0.910)19 (0.310) 15/394 2.7 27.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 45 (0.740)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment