breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,158,7790TG64.5% 26.4 / 31.9 31L97W (TTG→TGG) oxyRoxidative and nitrosative stress transcriptional regulator
*NC_000913_3_bme_pgi1,756,4970GT63.6% 30.2 / 34.4 33C267F (TGT→TTT) pykFpyruvate kinase I
*NC_000913_3_bme_pgi4,158,8850AC63.6% 33.8 / 33.8 33L132F (TTA→TTCoxyRoxidative and nitrosative stress transcriptional regulator
*NC_000913_3_bme_pgi3,546,3910CT53.8% 6.9 / 45.7 39intergenic (+192/‑168)nfuA/gntTFe/S biogenesis protein, putative scaffold/chaperone protein/gluconate transporter, high‑affinity GNT I system
*NC_000913_3_bme_pgi1,233,1570TA38.9% 11.7 / 20.2 18intergenic (‑127/+19)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
*NC_000913_3_bme_pgi1,233,1540TA36.8% 15.6 / 19.9 19intergenic (‑124/+22)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
*NC_000913_3_bme_pgi2,074,6910CG31.6% 55.1 / 38.0 38intergenic (+33/‑88)flu/yeeRCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
*NC_000913_3_bme_pgi4,262,2560GC30.3% 49.6 / 30.4 34S196T (AGC→ACC) dusAtRNA‑dihydrouridine synthase A
*NC_000913_3_bme_pgi4,262,2510GC29.4% 45.7 / 29.8 34G194G (GGG→GGCdusAtRNA‑dihydrouridine synthase A
*NC_000913_3_bme_pgi4,296,0610CT25.0% 77.1 / 29.0 49intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_000913_3_bme_pgi639,7240TG23.1% 66.4 / 26.9 39intergenic (+216/‑29)ahpC/ahpFalkyl hydroperoxide reductase, C22 subunit/alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
*NC_000913_3_bme_pgi1,449,0500TA23.1% 108.8 / 35.2 52intergenic (+32/+26)feaB/tynAphenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
*NC_000913_3_bme_pgi2,866,4870GC22.9% 74.1 / 20.8 35intergenic (+22/+72)ygbN/rpoSputative transporter/RNA polymerase, sigma S (sigma 38) factor
*NC_000913_3_bme_pgi2,866,4880GC22.9% 74.7 / 19.9 35intergenic (+23/+71)ygbN/rpoSputative transporter/RNA polymerase, sigma S (sigma 38) factor
*NC_000913_3_bme_pgi3,891,5020CG22.2% 80.3 / 22.8 36intergenic (+19/‑113)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
*NC_000913_3_bme_pgi4,088,0910GC22.2% 81.1 / 22.4 36intergenic (+34/+16)yiiG/frvRDUF3829 family lipoprotein/putative frv operon regulator; contains a PTS EIIA domain
*NC_000913_3_bme_pgi2,305,0850AG21.9% 86.1 / 17.8 41intergenic (+692/+23)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
*NC_000913_3_bme_pgi2,305,0860CG21.9% 102.8 / 25.5 41intergenic (+693/+22)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
*NC_000913_3_bme_pgi2,305,0870CT21.9% 83.7 / 18.8 41intergenic (+694/+21)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
*NC_000913_3_bme_pgi22,7730AT21.7% 49.9 / 11.0 23E128V (GAA→GTA) ileSisoleucyl‑tRNA synthetase

Marginal new junction evidence (lowest skew 10 of 213 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 4233518 =643 (17.360)30 (0.930) 22/212 0.6 5.1% intergenic (‑285/‑2389) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_bme_pgi 4233534 = 555 (17.240)intergenic (‑301/‑2373) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
* ? NC_000913_3_bme_pgi 4227808 =648 (17.490)34 (0.960) 22/234 0.7 5.1% coding (78/1632 nt) yjbB putative Na+/Pi‑cotransporter
?NC_000913_3_bme_pgi 4227825 = 645 (18.160)coding (95/1632 nt) yjbB putative Na+/Pi‑cotransporter
* ? NC_000913_3_bme_pgi = 4229386661 (17.840)28 (0.800) 20/230 0.9 4.3% noncoding (11/70 nt) REP312 (repetitive extragenic palindromic) element; contains 2 REP sequences REP312 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913_3_bme_pgi = 4229405 NA (NA)noncoding (30/70 nt) REP312 (repetitive extragenic palindromic) element; contains 2 REP sequences REP312 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913_3_bme_pgi 4226588 =554 (14.960)20 (0.540) 19/242 1.1 3.6% coding (2761/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
?NC_000913_3_bme_pgi 4226623 = 521 (14.180)coding (2796/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
* ? NC_000913_3_bme_pgi = 4227812648 (17.490)20 (0.560) 14/234 1.7 3.1% coding (82/1632 nt) yjbB putative Na+/Pi‑cotransporter
?NC_000913_3_bme_pgi = 4227819 645 (18.160)coding (89/1632 nt) yjbB putative Na+/Pi‑cotransporter
* ? NC_000913_3_bme_pgi = 4235432466 (12.580)16 (0.460) 13/230 1.8 3.5% noncoding (11/77 nt) REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913_3_bme_pgi = 4235448 NA (NA)noncoding (27/77 nt) REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913_3_bme_pgi = 4226475528 (14.250)20 (0.570) 13/232 1.8 3.8% coding (2648/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
?NC_000913_3_bme_pgi = 4226484 511 (14.510)coding (2657/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
* ? NC_000913_3_bme_pgi 4226590 =551 (14.870)13 (0.370) 12/230 2.0 2.5% coding (2763/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
?NC_000913_3_bme_pgi 4226621 = 514 (14.720)coding (2794/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
* ? NC_000913_3_bme_pgi 4235057 =580 (15.660)16 (0.460) 11/230 2.2 2.9% intergenic (‑1824/‑850) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_bme_pgi 4235081 = 526 (15.060)intergenic (‑1848/‑826) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
* ? NC_000913_3_bme_pgi 4226470 =528 (14.250)13 (0.370) 11/232 2.2 2.5% coding (2643/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
?NC_000913_3_bme_pgi 4226491 = 511 (14.510)coding (2664/3684 nt) metH homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent