New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 3654539 =33 (0.910)4 (0.120) 4/258 4.5 11.1% intergenic (+12/‑144) slp/dctR outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
?NC_000913_3_KO_pgi 3654568 = 34 (1.020)intergenic (+41/‑115) slp/dctR outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGTT  >  NC_000913_3_KO_pgi/3654426‑3654714
                                                                                                                                              |                                                                                                                                                  
gtgtgGGATTACGGCTATGATGCATTCTGGCAGGAACCGGGCGGGGGTGCGCCTTACTACACCAAAGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCGACGCAAGATGCAGATACTTTCTCAGGAACTGCATCATGCAtt                                                                                                                                              >  2:338379/2‑149 (MQ=255)
 tgtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTa                                                                                                                                             >  1:135857/1‑149 (MQ=255)
              cTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATa                                                                                                                                <  1:132927/149‑1 (MQ=255)
                    tGCATTCTGGCCGGAACCGGGATGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGt                                                                                                                          >  2:461858/1‑149 (MQ=255)
                                  aaCCGGGCTGGGGGGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattat                                                                                                            <  1:225504/149‑1 (MQ=255)
                                      gggCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatt                                                                                                        <  1:449793/149‑1 (MQ=255)
                                                         tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATacac                                                                                                                             >  1:401823/1‑109 (MQ=255)
                                                         tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATacac                                                                                                                             <  2:401823/109‑1 (MQ=255)
                                                          acacCAATACGGTGAGTCACGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCGGCATCATGCATTATAGCAAATTAAGACACAGTAAGCTAACTAATTGTCTTATGAGCCCTGTCCTGTTAAt                                                                                    >  2:540206/1‑149 (MQ=255)
                                                               aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                                     >  1:152143/1‑127 (MQ=255)
                                                               aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                                     <  2:152143/127‑1 (MQ=255)
                                                                    ggTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAACTTTGGc                                                                          >  1:248828/1‑149 (MQ=255)
                                                                      tGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGAGGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATATGCCCTGTCCTGTTAATTACCTTTGGCaa                                                                        >  1:290265/1‑149 (MQ=255)
                                                                             ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 >  2:492034/1‑149 (MQ=255)
                                                                             ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 <  1:492034/149‑1 (MQ=255)
                                                                             ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 >  2:269114/1‑149 (MQ=255)
                                                                                  cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCGTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATaaa                                                            <  2:294560/149‑1 (MQ=255)
                                                                                   acCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATGATAAAg                                                           >  1:481724/1‑149 (MQ=255)
                                                                                        aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAt                                                      <  2:472644/149‑1 (MQ=255)
                                                                                                     accccaccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCTTGTCCTGTTAATTACCTTTGGCAAACTGATTATAATGTTAATGTCCGCACCAGGa                                         <  2:216400/146‑1 (MQ=255)
                                                                                                             gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                                  <  2:549283/116‑1 (MQ=255)
                                                                                                             gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTGAATTACCTTTGGCAAACTGAt                                                                  >  1:549283/1‑116 (MQ=255)
                                                                                                             aaaaCATTCAGATGCCTTCTCAGGATCTGCATCATGCATTACATCGAATTAATACACAGTAATCTAACTATTATTATTCTAAGCCCGATCCTGTTAATTACCTTTGGCAATCTGATTATGAAGTTAATGTCCGCACCAGGAGTCGGTTa                                 <  1:540206/148‑1 (MQ=255)
                                                                                                              aaaGATGCAGATGCTTTCTCAGCATCTGCAGCATGCATTACATCAAATTAATACCCAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                                <  1:523012/149‑1 (MQ=255)
                                                                                                                  atgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCCCCAGGAGTCGGTTATGttt                            >  2:488316/1‑149 (MQ=255)
                                                                                                                      agatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGTGTATAATGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCttc                        >  1:283807/1‑148 (MQ=255)
                                                                                                                       gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                                    <  2:28678/88‑1 (MQ=255)
                                                                                                                       gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                                    >  1:28678/1‑88 (MQ=255)
                                                                                                                        atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAc                                                                                 <  2:121172/90‑1 (MQ=255)
                                                                                                                        atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAc                                                                                 >  1:121172/1‑90 (MQ=255)
                                                                                                                        atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtata                      >  1:215396/1‑149 (MQ=255)
                                                                                                                            tttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTa                  >  2:352935/1‑149 (MQ=255)
                                                                                                                                tcAGCATCTGCATCATGCATTACATAAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAg              <  2:135857/149‑1 (MQ=255)
                                                                                                                                 cAGCATCTTCATCATGCATTACATCAAATTAATACACCGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAgg             <  1:557537/149‑1 (MQ=255)
                                                                                                                                          catcatGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAACGTTACTGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATg    >  2:482699/1‑149 (MQ=255)
                                                                                                                                          catcatGCAGTACATCAAATTAATACACAGTAAGCTAACTAGTATTATTATAAGCCCTGTCCTGTTAATTACCGTTGGCAAACTGATTATAAAGATAATGTCCGCACCAGGAGTGGGTTATGTTTCTTATAATTACCAGGGATACGAta    >  2:409208/1‑148 (MQ=255)
                                                                                                                                            tcatGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGtt  <  2:178733/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGTT  >  NC_000913_3_KO_pgi/3654426‑3654714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.