breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 264,098 G→T 100% L50L (CTC→CTA ykfH ← uncharacterized protein
RA 275,065 G→A 100% T18I (ACC→ATC)  insH1 ← IS5 transposase and trans‑activator
RA 389,919:1 +T 100% intergenic (‑192/‑332) hemB ← / → yaiT 5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 482,230 G→A 100% P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 550,955 C→T 9.0% L173F (CTC→TTC)  ybcF → putative carbonate kinase
RA 1,158,559 C→T 100% Q231* (CAG→TAG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,196,232 C→T 20.2% T370T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,197,502 C→T 100% G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,224,239 A→T 11.5% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor
RA 1,544,022 1 bp→AA 100% coding (39/1389 nt) narU ← nitrate/nitrite transporter
RA 1,726,165 A→G 43.6% Y48C (TAC→TGC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
JC 1,726,433 Δ11 bp 33.3% coding (411‑421/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,726,530 G→C 29.1% A170P (GCG→CCG)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,734,464 G→C 20.3% T10T (ACG→ACC mepH → murein DD‑endopeptidase, space‑maker hydrolase
RA 1,774,576 G→A 100% G263E (GGG→GAG)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,106,741 A→T 100% S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T 100% A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A 100% Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A 100% E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 2,948,287 T→C 9.8% I242V (ATC→GTC)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase
RA 2,948,288 G→A 8.4% D241D (GAC→GAT amiC ← N‑acetylmuramoyl‑L‑alanine amidase
RA 2,976,638 T→G 69.2% S14A (TCA→GCA)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 2,976,684 G→T 19.0% S29I (AGC→ATC)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,183,136 G→T 72.8% R637L (CGT→CTT)  rpoB → RNA polymerase, beta subunit
RA 4,187,572 G→T 18.9% M747I (ATG→ATT rpoC → RNA polymerase, beta prime subunit
RA 4,296,042 C→T 16.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,362:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,698 C→A 100% A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_tpiA 365890 366373 484 10 [8] [8] 9 [lacZ] [lacZ]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 129734 =46 (1.320)3 (0.090) 3/264 NT 6.3% coding (1527/1854 nt) yacH DUF3300 family protein
?NC_000913_3_hsa_tpiA 129754 = 45 (1.350)coding (1507/1854 nt) yacH DUF3300 family protein
* ? NC_000913_3_hsa_tpiA = 35715539 (1.120)3 (0.090) 3/264 NT 7.4% coding (985/1284 nt) codA cytosine/isoguanine deaminase
?NC_000913_3_hsa_tpiA = 357165 38 (1.140)coding (995/1284 nt) codA cytosine/isoguanine deaminase
* ? NC_000913_3_hsa_tpiA 1207790 =7 (0.200)16 (0.520) 15/244 NT 66.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_hsa_tpiA 1209619 = 10 (0.330)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_hsa_tpiA = 12078059 (0.260)22 (0.720) 20/244 NT 71.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_hsa_tpiA = 1209602 10 (0.330)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_hsa_tpiA = 12994980 (0.000)48 (1.420) 38/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_hsa_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein