New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3227830	52 (1.070)	4 (0.090)	4/274	6.8	7.3%	coding (30/1071 nt)	ygjJ	putative periplasmic protein
	?	NC_000913_3_pae_tpiA	= 3227835	53 (1.160)					coding (35/1071 nt)	ygjJ	putative periplasmic protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATGAGCAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATA  >  NC_000913_3_pae_tpiA/3227683‑3227978                                                                                                                                                    |                                                                                                                                                     aTGAGCAGGCATTTTCCCTCTTCCTTTTTTAGTATAGGGAAAATAGATTCTGCCTTTATATTTTTATAATTCCAACGATAAAAGGCTGTACTTTTTCTTTTGCTCATGGATTAACACAATTCATTTAATCATTTCACCATGCAGAGCAt                                                                                                                                                     >  2:796481/1‑149 (MQ=255)      cAGGCATTTTCCCTCTTCCTTGATCAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACtt                                                                                                                                                <  2:41049/149‑1 (MQ=255)           aTTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTCATAATTGCAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTTctct                                                                                                                                           <  2:647318/149‑1 (MQ=255)              ttCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACCCAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGcc                                                                                                                                        <  2:570130/149‑1 (MQ=255)                   tctTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGtttt                                                                                                                                   <  2:843974/149‑1 (MQ=255)                   tctTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGtttt                                                                                                                                   <  1:552814/149‑1 (MQ=255)                     ttCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTACTGGATTAACACAATGAAATTAATCACTGCACCATGCGGAGCATTACTTGCTCTGCCGTTTTGc                                                                                                                                 >  1:221747/1‑149 (MQ=255)                      tCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCt                                                                                                                                >  2:740959/1‑149 (MQ=255)                       ccTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTa                                                                                                                               <  1:350167/149‑1 (MQ=255)                       ccTGGATGAGGAGAGGGAAAATAGAAGCGGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTAATTGATCTGACGttgatgac                                                                                                                               >  1:594571/1‑143 (MQ=255)                        cTGGATGAGGAGAGGGAAAATAGTTTCTGCATTTACATTCTTACGCTTACTACTATAAAAGGCTGTACTTTTTCTTTAGCTCAGGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAc                                                                                                                              <  1:66311/149‑1 (MQ=255)                            aTGAGGAGAGGGAAAATAGTTTCTGCCTTCATATTTTTATAATTACAACGATAAGAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCt                                                                                                                          <  1:731857/149‑1 (MQ=255)                                    agGGAAAGTCGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTTATGGATTAACACAAGGAAATTAATCACTGCACCATGCAGAGCATTACTTGGTCTGCCGGTTTGCTTCACCTTTTCTGCg                                                                                                                  >  1:601063/1‑149 (MQ=255)                                      ggAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATTACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGc                                                                                                                >  1:283485/1‑149 (MQ=255)                                       gAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCa                                                                                                               <  1:638820/149‑1 (MQ=255)                                         aaaTAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTAATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAACACTTTACTGCGCCAgg                                                                                                             >  2:131999/1‑149 (MQ=255)                                            tAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATAGATTAACAAAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCACTGCCGTTTTGATACGCCATTTCTGCGGCAGgata                                                                                                          >  2:850954/1‑147 (MQ=255)                                              gTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGggaag                                                                                                        >  1:257026/1‑149 (MQ=255)                                              gTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTCCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGgagcat                                                                                                        >  1:258323/1‑146 (MQ=255)                                                ttCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAgaagaa                                                                                                      <  2:777247/149‑1 (MQ=255)                                                 tCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGaagaag                                                                                                     <  1:515327/149‑1 (MQ=255)                                                   tGCCTTTATATTTTTATAATTACAACGATAAAATTCTGTACTTTTTATTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTCCGCCTTTTCTGCGGCATGagaagaccaa                                                                                                   >  2:311062/1‑145 (MQ=255)                                                    gCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGGTCAGGCATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCAc                                                                                                  <  1:450396/149‑1 (MQ=255)                                                               tttATAATTACAACGAGAAAAGGCTGTATTTTTTCTTTAGCTCATGGATTAACACAATGGAATTAATCACTGGACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGaa                                                                                       <  2:138037/149‑1 (MQ=255)                                                               tttATAATTACAACGAAAAAAGGCTGTACTTTTGCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGaa                                                                                       <  2:66974/149‑1 (MQ=255)                                                                   taATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATg                                                                                   <  2:584752/149‑1 (MQ=255)                                                                   taATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATAAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATg                                                                                   >  1:594880/1‑149 (MQ=255)                                                                     aTTAAAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGAGTAACACAATGAAATTAAGCACTGCACCCTGCATAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGTAGAAGAAGCACGTCCGTCAGAACATgac                                                                                 <  2:115995/149‑1 (MQ=255)                                                                      ttACAACTATAAAAGGCTGTACTTTCTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacg                                                                                <  1:492631/149‑1 (MQ=255)                                                                       tACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacga                                                                               <  1:18575/149‑1 (MQ=255)                                                                         cactgATAAAAGGCTGCACTTCTTCTTTAGTTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTTCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGaca                                                                             <  1:373030/145‑1 (MQ=255)                                                                         cAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAAAACCCTGCGGTGGATCGCTCCACAATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGaca                                                                             <  1:374514/149‑1 (MQ=255)                                                                             gATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAACAAGCGCGTCCGACAGAACATAACGAAACaaa                                                                         >  1:74281/1‑149 (MQ=255)                                                                             gATAAAAGGCCTTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACaaa                                                                         <  1:91129/149‑1 (MQ=255)                                                                               tAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACaaaaa                                                                       <  1:797035/149‑1 (MQ=255)                                                                                            tttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCTGCAGAACATGACGACACAAAAACACCCGCCATAAc                                                          >  2:545604/1‑149 (MQ=255)                                                                                             ttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTACGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTAcc                                                         >  2:93250/1‑149 (MQ=255)                                                                                               ttCTTTAGCTCATGGATTAACACAATGAAATTCATCACTGCACCATGGATAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACTACACAAAAACACCCGCAATTACCTc                                                       <  2:655010/149‑1 (MQ=255)                                                                                               ttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTc                                                       <  2:747972/149‑1 (MQ=255)                                                                                                        tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACAtcttc                                              >  1:112740/1‑149 (MQ=255)                                                                                                        tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCACGCTAAGGAGAAAAAAACCATGAGTATGACGACACAAAAACACCCTCAATTACCTCGACAtcttc                                              <  1:839159/149‑1 (MQ=255)                                                                                                           tGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGCCATCTTCTcc                                           >  1:342754/1‑149 (MQ=255)                                                                                                                   cacaATGAAAGTAATCACTGCACCATGCAGGGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCAttgc                                   <  2:435336/149‑3 (MQ=255)                                                                                                                    acaATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCg                                  >  2:2659/1‑149 (MQ=255)                                                                                                                      aaTGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtt                                <  1:671128/149‑1 (MQ=255)                                                                                                                                   aCTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAAAACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAAtt                   >  1:575337/1‑149 (MQ=255)                                                                                                                                     tGCACCATGCAGAGCATGACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCGCCTTCACTCCGTTTTTACGGCGAATTAg                 >  2:670726/1‑149 (MQ=255)                                                                                                                                          cATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCCGGACATGACGACACGAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGtt            >  1:756183/1‑149 (MQ=255)                                                                                                                                              cAGAGCATTACTTGCCCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCACGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTtggtg        <  1:503318/149‑1 (MQ=255)                                                                                                                                                 agCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTACGGCAAAACATGACGACACAACAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGGTTTTACGGCGAATTAGGGGTTGGTGGat     <  1:169570/149‑1 (MQ=255)                                                                                                                                                 agCATTACTTGCTCTGACGTTTTGCTACGCCTCTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGGTTTGACTGCGAATTAGGGGTTGGTGGag     >  2:814890/1‑148 (MQ=255)                                                                                                                                                    aTTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGAGGatata  >  2:255690/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ATGAGCAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATA  >  NC_000913_3_pae_tpiA/3227683‑3227978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.