New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 322783052 (1.070)4 (0.090) 4/274 6.8 7.3% coding (30/1071 nt) ygjJ putative periplasmic protein
?NC_000913_3_pae_tpiA = 3227835 53 (1.160)coding (35/1071 nt) ygjJ putative periplasmic protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATATG  >  NC_000913_3_pae_tpiA/3227688‑3227980
                                                                                                                                                   |                                                                                                                                                 
cAGGCATTTTCCCTCTTCCTTGATCAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACtt                                                                                                                                                  <  2:41049/149‑1 (MQ=255)
     aTTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTCATAATTGCAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTTctct                                                                                                                                             <  2:647318/149‑1 (MQ=255)
        ttCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACCCAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGcc                                                                                                                                          <  2:570130/149‑1 (MQ=255)
             tctTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGtttt                                                                                                                                     <  2:843974/149‑1 (MQ=255)
             tctTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGtttt                                                                                                                                     <  1:552814/149‑1 (MQ=255)
               ttCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTACTGGATTAACACAATGAAATTAATCACTGCACCATGCGGAGCATTACTTGCTCTGCCGTTTTGc                                                                                                                                   >  1:221747/1‑149 (MQ=255)
                tCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCt                                                                                                                                  >  2:740959/1‑149 (MQ=255)
                 ccTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTa                                                                                                                                 <  1:350167/149‑1 (MQ=255)
                 ccTGGATGAGGAGAGGGAAAATAGAAGCGGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTAATTGATCTGACGttgatgac                                                                                                                                 >  1:594571/1‑143 (MQ=255)
                  cTGGATGAGGAGAGGGAAAATAGTTTCTGCATTTACATTCTTACGCTTACTACTATAAAAGGCTGTACTTTTTCTTTAGCTCAGGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAc                                                                                                                                <  1:66311/149‑1 (MQ=255)
                      aTGAGGAGAGGGAAAATAGTTTCTGCCTTCATATTTTTATAATTACAACGATAAGAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCt                                                                                                                            <  1:731857/149‑1 (MQ=255)
                              agGGAAAGTCGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTTATGGATTAACACAAGGAAATTAATCACTGCACCATGCAGAGCATTACTTGGTCTGCCGGTTTGCTTCACCTTTTCTGCg                                                                                                                    >  1:601063/1‑149 (MQ=255)
                                ggAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATTACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGc                                                                                                                  >  1:283485/1‑149 (MQ=255)
                                 gAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCa                                                                                                                 <  1:638820/149‑1 (MQ=255)
                                   aaaTAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTAATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAACACTTTACTGCGCCAgg                                                                                                               >  2:131999/1‑149 (MQ=255)
                                      tAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATAGATTAACAAAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCACTGCCGTTTTGATACGCCATTTCTGCGGCAGgata                                                                                                            >  2:850954/1‑147 (MQ=255)
                                        gTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGggaag                                                                                                          >  1:257026/1‑149 (MQ=255)
                                        gTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTCCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGgagcat                                                                                                          >  1:258323/1‑146 (MQ=255)
                                          ttCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAgaagaa                                                                                                        <  2:777247/149‑1 (MQ=255)
                                           tCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGaagaag                                                                                                       <  1:515327/149‑1 (MQ=255)
                                             tGCCTTTATATTTTTATAATTACAACGATAAAATTCTGTACTTTTTATTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTCCGCCTTTTCTGCGGCATGagaagaccaa                                                                                                     >  2:311062/1‑145 (MQ=255)
                                              gCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGGTCAGGCATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCAc                                                                                                    <  1:450396/149‑1 (MQ=255)
                                                         tttATAATTACAACGAGAAAAGGCTGTATTTTTTCTTTAGCTCATGGATTAACACAATGGAATTAATCACTGGACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGaa                                                                                         <  2:138037/149‑1 (MQ=255)
                                                         tttATAATTACAACGAAAAAAGGCTGTACTTTTGCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGaa                                                                                         <  2:66974/149‑1 (MQ=255)
                                                             taATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATg                                                                                     <  2:584752/149‑1 (MQ=255)
                                                             taATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATAAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATg                                                                                     >  1:594880/1‑149 (MQ=255)
                                                               aTTAAAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGAGTAACACAATGAAATTAAGCACTGCACCCTGCATAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGTAGAAGAAGCACGTCCGTCAGAACATgac                                                                                   <  2:115995/149‑1 (MQ=255)
                                                                ttACAACTATAAAAGGCTGTACTTTCTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacg                                                                                  <  1:492631/149‑1 (MQ=255)
                                                                 tACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacga                                                                                 <  1:18575/149‑1 (MQ=255)
                                                                   cactgATAAAAGGCTGCACTTCTTCTTTAGTTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTTCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGaca                                                                               <  1:373030/145‑1 (MQ=255)
                                                                   cAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAAAACCCTGCGGTGGATCGCTCCACAATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGaca                                                                               <  1:374514/149‑1 (MQ=255)
                                                                       gATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAACAAGCGCGTCCGACAGAACATAACGAAACaaa                                                                           >  1:74281/1‑149 (MQ=255)
                                                                       gATAAAAGGCCTTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACaaa                                                                           <  1:91129/149‑1 (MQ=255)
                                                                         tAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACaaaaa                                                                         <  1:797035/149‑1 (MQ=255)
                                                                                      tttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCTGCAGAACATGACGACACAAAAACACCCGCCATAAc                                                            >  2:545604/1‑149 (MQ=255)
                                                                                       ttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTACGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTAcc                                                           >  2:93250/1‑149 (MQ=255)
                                                                                         ttCTTTAGCTCATGGATTAACACAATGAAATTCATCACTGCACCATGGATAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACTACACAAAAACACCCGCAATTACCTc                                                         <  2:655010/149‑1 (MQ=255)
                                                                                         ttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTc                                                         <  2:747972/149‑1 (MQ=255)
                                                                                                  tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACAtcttc                                                >  1:112740/1‑149 (MQ=255)
                                                                                                  tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCACGCTAAGGAGAAAAAAACCATGAGTATGACGACACAAAAACACCCTCAATTACCTCGACAtcttc                                                <  1:839159/149‑1 (MQ=255)
                                                                                                     tGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGCCATCTTCTcc                                             >  1:342754/1‑149 (MQ=255)
                                                                                                             cacaATGAAAGTAATCACTGCACCATGCAGGGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCAttgc                                     <  2:435336/149‑3 (MQ=255)
                                                                                                              acaATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCg                                    >  2:2659/1‑149 (MQ=255)
                                                                                                                aaTGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtt                                  <  1:671128/149‑1 (MQ=255)
                                                                                                                             aCTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAAAACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAAtt                     >  1:575337/1‑149 (MQ=255)
                                                                                                                               tGCACCATGCAGAGCATGACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCGCCTTCACTCCGTTTTTACGGCGAATTAg                   >  2:670726/1‑149 (MQ=255)
                                                                                                                                    cATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCCGGACATGACGACACGAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGtt              >  1:756183/1‑149 (MQ=255)
                                                                                                                                        cAGAGCATTACTTGCCCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCACGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTtggtg          <  1:503318/149‑1 (MQ=255)
                                                                                                                                           agCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTACGGCAAAACATGACGACACAACAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGGTTTTACGGCGAATTAGGGGTTGGTGGat       <  1:169570/149‑1 (MQ=255)
                                                                                                                                           agCATTACTTGCTCTGACGTTTTGCTACGCCTCTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGGTTTGACTGCGAATTAGGGGTTGGTGGag       >  2:814890/1‑148 (MQ=255)
                                                                                                                                              aTTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGAGGatata    >  2:255690/1‑149 (MQ=255)
                                                                                                                                               ttACTTGCTCTGCCGTTTTGCTACGGCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGatatat   <  2:375289/149‑1 (MQ=255)
                                                                                                                                               ttACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGatatat   <  1:93250/149‑1 (MQ=255)
                                                                                                                                                tACTTGCTCTGCCGTTTCGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATATg  <  1:404911/149‑1 (MQ=255)
                                                                                                                                                gaCTTGCTCGGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATATg  <  2:323771/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATATG  >  NC_000913_3_pae_tpiA/3227688‑3227980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.