breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,484,228 C→T Q390* (CAG→TAG)  hrpA → putative ATP‑dependent helicase
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,106,741 A→T S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,296,297 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 3423741–3424538 3424538 1–798 8 [6] [6] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)31 (1.080) 29/282 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913_3_pae_tpiA 1978503 =NA (NA)25 (0.850) 22/290 0.4 96.2% intergenic (‑306/+15) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913_3_pae_tpiA = 2490627 1 (0.030)coding (1324/1695 nt) oxc oxalyl CoA decarboxylase, ThDP‑dependent
* ? NC_000913_3_pae_tpiA = 1979269NA (NA)20 (0.680) 18/288 0.7 95.3% intergenic (‑54/‑484) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913_3_pae_tpiA 2490618 = 1 (0.030)coding (1333/1695 nt) oxc oxalyl CoA decarboxylase, ThDP‑dependent
* ? NC_000913_3_pae_tpiA = 3427065NA (NA)6 (0.220) 6/272 2.8 NA intergenic (‑32/+11) alaU/ileU tRNA‑Ala/tRNA‑Ile
?NC_000913_3_pae_tpiA = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile