New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	319208 =	48 (0.750)	4 (0.060)	4/246	NT	7.7%	coding (534/1815 nt)	PP_0264	sensor histidine kinase
	?	NC_002947	319244 =	49 (0.790)					coding (498/1815 nt)	PP_0264	sensor histidine kinase

GTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGCCGACGAA  >  NC_002947/319061‑319354                                                                                                                                                    |                                                                                                                                                   accttggccgcctcgaactgggacaagcacgacagcttcgtcggccgaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccacggcaccacctcggccaagcgcggctactactttGc                                                                                                                                                   <  4:169704‑M1/2‑1 (MQ=255)   cttggccgcctcgaactgggacaagcacgacagcttcgtcggccgaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCcg                                                                                                                                                 <  8:255627‑M1/4‑1 (MQ=255)   cttggccgcctcgaactgggacaagcacgacagcttcgtcggccgaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCcg                                                                                                                                                 <  4:67413‑M1/4‑1 (MQ=255)    ggTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCcga                                                                                                                                                <  2:147939/149‑1 (MQ=255)     gTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCcgac                                                                                                                                               <  6:83954/149‑1 (MQ=255)     gTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCcgac                                                                                                                                               >  5:363483/1‑149 (MQ=255)     gTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCcgac                                                                                                                                               >  5:83954/1‑149 (MQ=255)        aGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCcgacga                                                                                                                                             <  5:125637/148‑1 (MQ=255)        aGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGAC‑TTGACCACCAGCACGCCGACGATc                                                                                                                                           <  7:177211/149‑1 (MQ=255)           gTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCg                                                                                                                                         >  8:164049/1‑149 (MQ=255)                     cACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTcg                                                                                                                               >  5:142992/1‑149 (MQ=255)                           gcacgacagcttcgtcggccgaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCCGACGATCCGCTCGCGGTCGCGCACg                                                                                                                         <  6:356484‑M1/28‑1 (MQ=255)                                     gggCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCa                                                                                                                >  7:299414/1‑148 (MQ=255)                                          gcgAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATc                                                                                                                                           >  2:280275/1‑116 (MQ=255)                                           ggccgaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCCGACGATCCGCTCTCGGTCGCGCACGGCCGCGGCAaagaag                                                                                                          >  5:356484‑M1/106‑148 (MQ=255)                                               gaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAaag                                                                                                             <  6:114432‑M1/40‑1 (MQ=255)                                               gaaacttcgccttccgcccttacttcaacgaagccatggccggccaccttggccgcttcttcggccagggcaccacctcggccaagcgcggctacttctttGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAaag                                                                                                             >  5:114432‑M1/102‑141 (MQ=255)                                                    gATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCt                                                                                                >  3:81394/1‑149 (MQ=255)                                                      tGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTg                                                                                              <  7:164049/149‑1 (MQ=255)                                                          cACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGcc                                                                                           >  3:199347/1‑148 (MQ=255)                                                          cACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGcc                                                                                           >  3:181791/1‑148 (MQ=255)                                                          cACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCg                                                                                          <  6:142992/149‑1 (MQ=255)                                                               cATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAggtg                                                                                     >  4:160566/1‑149 (MQ=255)                                                                   gtgATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGgtggtg                                                                                  <  3:160566/148‑1 (MQ=255)                                                                      aTCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAgg                                                                                       >  6:231669/1‑140 (MQ=255)                                                                      aTCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAgg                                                                                       <  5:231669/140‑1 (MQ=255)                                                                         ggTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTgg                                                                            <  8:76373/148‑1 (MQ=255)                                                                          gTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGcc                                                                          >  3:182205/1‑149 (MQ=255)                                                                                     ctgctCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGCTCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGGGGTGCCCTGGCCGAAGAAGCGGc                                                               >  8:12299/1‑149 (MQ=255)                                                                                        ctCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCaa                                                            <  4:199347/149‑1 (MQ=255)                                                                                           gggCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGt                                                         <  4:182205/149‑1 (MQ=255)                                                                                                   ggCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCgaa                                                                       <  1:359754/127‑1 (MQ=255)                                                                                                   ggCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCgaa                                                                       >  2:359754/1‑127 (MQ=255)                                                                                                   ggCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTggccggc                                                  >  5:296453/1‑148 (MQ=255)                                                                                                   ggCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTggccggc                                                  >  3:302447/1‑148 (MQ=255)                                                                                                       ccACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTg                                                                                              >  6:46415/1‑100 (MQ=255)                                                                                                       ccACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTg                                                                                              <  5:46415/100‑1 (MQ=255)                                                                                                       ccACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATgg                                             >  6:406825/1‑149 (MQ=255)                                                                                                           aGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTc                                         >  4:95599/1‑149 (MQ=255)                                                                                                                   ggTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAg                                  >  1:174711/1‑148 (MQ=255)                                                                                                                    gtgtGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGaa                                   <  3:186629/146‑1 (MQ=255)                                                                                                                    gtgtGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGaa                                   >  4:186629/1‑146 (MQ=255)                                                                                                                     tgtgGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTaa                               <  3:95599/149‑1 (MQ=255)                                                                                                                       tgGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGGGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCg                                        >  1:288249/1‑138 (MQ=255)                                                                                                                       tgGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCg                                        <  2:288249/138‑1 (MQ=255)                                                                                                                       tgGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAgg                             <  4:81394/149‑1 (MQ=255)                                                                                                                       tgGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAgg                             <  2:174711/149‑1 (MQ=255)                                                                                                                          tCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCg                          >  3:169704/1‑149 (MQ=255)                                                                                                                              gaTCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAg                      >  3:67413/1‑149 (MQ=255)                                                                                                                                   aCTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGaa                 <  4:181791/149‑1 (MQ=255)                                                                                                                                    cTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAg                >  3:298164/1‑149 (MQ=255)                                                                                                                                         accaccAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCg           <  6:296453/149‑1 (MQ=255)                                                                                                                                          ccaccaGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCgg          >  7:158578/1‑149 (MQ=255)                                                                                                                                          ccaccaGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCgg          >  5:145098/1‑149 (MQ=255)                                                                                                                                           caccaGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGc         >  5:220814/1‑149 (MQ=255)                                                                                                                                            accaGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGcc        >  5:263125/1‑149 (MQ=255)                                                                                                                                                  aCGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGCcgacg    >  1:46994/1‑147 (MQ=255)                                                                                                                                                  aCGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGCCGACGaa  <  6:363483/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGATGACCACGCCATTGTGATCGGTCAGCAACAGCTGCTCGGGCGTACGGCCCCACAGGGTTTCGGTGTGGTCGAGATCGACTTTGACCACCAGCACGCCGACGATCCGCTCGCGGTCGCGCACGGCCGCGGCAAAGAAGTAGCCGCGCTTGGCCGAGGTGGTGCCCTGGCCGAAGAAGCGGCCAAGGTGGCCGGCCATGGCTTCGTTGAAGTAAGGGCGGAAGGCGAAGTTTCGGCCGACGAA  >  NC_002947/319061‑319354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.