New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	799772 =	47 (0.730)	3 (0.050)	3/246	NT	5.8%	coding (701/969 nt)	ispB	octaprenyl diphosphate synthase
	?	NC_002947	799809 =	52 (0.840)					coding (664/969 nt)	ispB	octaprenyl diphosphate synthase

GGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGG  >  NC_002947/799624‑799916                                                                                                                                                     |                                                                                                                                                 ggTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGc                                                                                                                                                  <  7:209422/149‑1 (MQ=255)  gtgtATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGcc                                                                                                                                                 <  7:27208/149‑1 (MQ=255)     tATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGccca                                                                                                                                               >  2:370540/1‑147 (MQ=255)               cgcCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtc                                                                                                                                     <  1:335088/148‑1 (MQ=255)                gcCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtcg                                                                                                                                    >  8:125612/1‑148 (MQ=255)                  ccGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCa                                                                                                                                 <  1:370540/149‑1 (MQ=255)                   cGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCAc                                                                                                                                <  7:125612/149‑1 (MQ=255)                    gATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCAGCGTAGGTTAGACATCTACAAGGTCGTCAcc                                                                                                                               >  6:10520/1‑149 (MQ=255)                        cttcGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGAc                                                                                                                            <  1:253868/148‑1 (MQ=255)                         ttcGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTACCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGt                                                                                                                          >  5:49705/1‑149 (MQ=255)                                  aaCGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGcc                                                                                                                   >  1:326152/1‑147 (MQ=255)                                      tggcccaggccaccgacgagcagcgcgaagccctgcgtacctttggcgaccatctgggcgtggccttccagctggtcgacgacctgctggactacaagggtgactcgcagaCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGt                                                                                                             >  4:27701‑M1/112‑149 (MQ=255)                                       gAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGACTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTc                                                                                                            >  5:288602/1‑149 (MQ=255)                                         aTCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTACCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTg                                                                                                          <  6:49705/149‑1 (MQ=255)                                            tGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGc                                                                                                         >  5:122264/1‑147 (MQ=255)                                             gTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtcgt                                                                                                                                   <  3:152250/120‑1 (MQ=255)                                              ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGa                                                                                                       <  7:174597/147‑1 (MQ=255)                                              ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGt                                                                                                     >  7:286911/1‑149 (MQ=255)                                              ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGt                                                                                                     >  3:320152/1‑149 (MQ=255)                                              ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCCGCAGTGCCTTCGCGCATGGTGTAGATCAGCGTCAGGGTAGGATAAACATCATAAAGCTCGTCACAGACGTTCTTGACCAGGCTCTGCGAga                                                                                                     >  8:376916/1‑148 (MQ=255)                                                 cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCATGGTAGTTTTTAAATCAGACAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAc                                                                                                  >  8:184497/1‑149 (MQ=255)                                                 cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCa                                                                                                                                 <  3:283628/118‑1 (MQ=255)                                                 cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCAAGGTCGTCa                                                                                                                                 >  4:283628/1‑118 (MQ=255)                                                  aGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAcc                                                                                                 >  7:7644/1‑149 (MQ=255)                                                   gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTTCAAACAGCAAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTc                                                                                                    >  6:59752/1‑145 (MQ=255)                                                   gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTc                                                                                                    <  5:59752/145‑1 (MQ=255)                                                   gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGGCATCGAAGGATAAAAATAAAAAAGTTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAccc                                                                                                >  6:37668/1‑149 (MQ=255)                                                     ttttCCCGGCCACCCTTTTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCtt                                                                                              <  6:288602/147‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGGCGGCTGCTCGGCAGTGCCTTCGCGCATGGTTTAGATCAGCGGCAGGGGAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                                             <  2:290544/146‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                                             <  7:184497/146‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                                             <  4:320152/146‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                                           <  8:286911/148‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                                           <  2:326152/148‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                                           <  1:258289/148‑1 (MQ=255)                                                         ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCATGGTAGTTTTCAAATCTAAAAAGTCGTCACCGACGtt                                                                                                                         >  8:35333/1‑118 (MQ=255)                                                          cAGGCCAGCGTTCTGGATCGCCTGGCGTACCAGCGCCGCATGCTCGGCAGTGGCTTCGCGCATGGTGTAGTTCAGCGGGATGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGt                                                                                         <  5:291335/149‑1 (MQ=255)                                                          cAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCATCGAAGTATAAAAAAAAAAAAAATCGTAACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGt                                                                                         >  4:377130/1‑149 (MQ=255)                                                             gCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGGCTGCTCGGCTGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCca                                                                                      <  6:122264/149‑1 (MQ=255)                                                                aCCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCcagca                                                                                   <  8:7644/149‑1 (MQ=255)                                                                 cccTTCTGGATCGCCTGGCGTACCAGCGCCTCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCTGCGGCATGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCcagcag                                                                                  <  5:113145/149‑1 (MQ=255)                                                                         gATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGa                                                                          <  5:10520/149‑1 (MQ=255)                                                                                   gcgaccatctgggcgtggccttccagctggtcgacgacctgctggactacaagggtgactcgcagaCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGc                                                                                                         >  3:230522‑M1/67‑108 (MQ=255)                                                                                   gcgaccatctgggcgtggccttccagctggtcgacgacctgctggactacaagggtgactcgcagaCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGc                                                                                                         <  4:230522‑M1/42‑1 (MQ=255)                                                                                    gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAg                                                               <  3:104065/149‑1 (MQ=255)                                                                                    gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAg                                                               <  7:376916/149‑1 (MQ=255)                                                                                       ccAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGcc                                                            <  3:377130/149‑1 (MQ=255)                                                                                                     tCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTc                                              >  5:205443/1‑149 (MQ=255)                                                                                                                         ggTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCtt                          >  3:303502/1‑149 (MQ=255)                                                                                                                            gtAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAg                               >  6:225222/1‑141 (MQ=255)                                                                                                                            gtAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAg                               <  5:225222/141‑1 (MQ=255)                                                                                                                             tAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGGCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTcgcg                      <  8:378459/149‑1 (MQ=255)                                                                                                                                 tCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctgc                  >  2:350702/1‑149 (MQ=255)                                                                                                                                          gtgTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGGCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTg         <  5:359350/147‑1 (MQ=255)                                                                                                                                                gTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGgtcg                                                                              <  3:72908/74‑1 (MQ=255)                                                                                                                                                gTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGgtcg                                                                              >  4:72908/1‑74 (MQ=255)                                                                                                                                                 tttGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTggg  <  4:303502/149‑1 (MQ=255)                                                                                                                                                 tttGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTggg  >  5:14148/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGG  >  NC_002947/799624‑799916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.