New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 965454	45 (0.860)	4 (0.080)	4/250	NT	9.5%	coding (436/768 nt)	ptxC	phosphonate transport system permease PtxC
	?	NC_002947	= 965474	34 (0.700)					coding (456/768 nt)	ptxC	phosphonate transport system permease PtxC

TTGCAAGGGGCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTACAGCTTGTACCGCTG  >  NC_002947/965307‑965596                                                                                                                                                     |                                                                                                                                               ttGCAAGGGGCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCgttt                                                                                                                                               <  7:5953/149‑1 (MQ=255)     aaGGGGCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCgtttgttt                                                                                                                                           >  2:159440/1‑149 (MQ=255)      aGGGGCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGCTACCGATGACTGTGCTGGCGGATGGGATGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGATGCCGTGCTGGGGCgtttgttt                                                                                                                                           >  1:252236/1‑149 (MQ=255)       ggggCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGc                                                                                                                                         <  2:58640/149‑1 (MQ=255)           ccGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAg                                                                                                                                     <  6:60911/149‑1 (MQ=255)           ccGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACGGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAg                                                                                                                                     <  8:182343/149‑1 (MQ=255)            cgcgcgTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAgg                                                                                                                                    >  1:170088/1‑149 (MQ=255)               gcgTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGgcgc                                                                                                                                 <  3:27790/149‑1 (MQ=255)                cgTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGTGGCGTTTGTTTGCCGAGGCGCt                                                                                                                                <  3:219046/149‑1 (MQ=255)                    ggcggctCAATGCCTTGCGGGCCATTCCTCAACTGGTGTGGGC‑CCCGCGGACGGTGCGGCCGGCGGGGCTGGGGCCGAAGCCCGGTACCCGGGCTGTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTga                                                                                                                             <  1:247875/144‑1 (MQ=255)                       tgctCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGc                                                                                                                                         <  4:312540/133‑1 (MQ=255)                       tgctCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGc                                                                                                                                         >  3:312540/1‑133 (MQ=255)                       tgctCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGgc                                                                                                                                   >  3:7761/1‑139 (MQ=255)                       tgctCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGgc                                                                                                                                   <  4:7761/139‑1 (MQ=255)                                 cTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGTGTTTGTTTGCCGAGgc                                                                                                                                   <  3:92780/129‑1 (MQ=255)                                 cTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGAGTTTGTTTGCCGAGgc                                                                                                                                   >  4:92780/1‑129 (MQ=255)                                       tGCCATTCCTGCACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCcgg                                                                                                         <  4:65772/149‑1 (MQ=255)                                         ccATTCCTGAACTGGTGTGGGG‑TTCGCTGACTGTGGATGCGGCTGGGCTGGGGCGGAATGCGGGTACCCTGGCTGTGGCGTTGCTCACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCGCCGCCCGAGCcggc                                                                                                        <  5:103689/148‑1 (MQ=255)                                           aTTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCcggcggc                                                                                                     >  3:160518/1‑149 (MQ=255)                                               cTGAACTGTTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTctcaagcgcctcggcaaacaaacgccccagcacgccagcggt                                                                                                      <  5:323405‑M1/144‑43 (MQ=255)                                               cTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCGTGGGGGTTataaagcgccacggcaaaaaaacgccccagcacgccaccggtgaga                                                                                                  >  6:323405‑M1/1‑102 (MQ=255)                                                tGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAg                                                                                                                                     >  5:275415/1‑112 (MQ=255)                                                tGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAg                                                                                                                                     <  6:275415/112‑1 (MQ=255)                                                       gtgtGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCTCACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGATCGCCCCGCCCGAGCCGGCGGCGGCGATCCGttt                                                                                         >  4:258107/1‑149 (MQ=255)                                                        tgtgGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCgttt                                                                                                                                               <  7:134912/94‑1 (MQ=255)                                                        tgtgGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCgttt                                                                                                                                               >  8:134912/1‑94 (MQ=255)                                                                aGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAggggg                                                                                 >  8:237189/1‑149 (MQ=255)                                                                aGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAggggg                                                                                 <  6:52882/149‑1 (MQ=255)                                                                     tGACTGTGCTGGCGGCTGGGCGGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGc                                                                            <  2:252236/149‑1 (MQ=255)                                                                            gCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTgg                                                                     >  2:312169/1‑149 (MQ=255)                                                                             cTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTggc                                                                    >  6:225881/1‑149 (MQ=255)                                                                                gcggcTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTggcggc                                                                 <  3:222186/149‑1 (MQ=255)                                                                                     tgggctgggGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTctcaagcgcctcggcaaacaaacgccc                                                                                                                     <  5:313949‑M1/92‑28 (MQ=255)                                                                                     tgggctgggGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTctcaagcgcctcggcaaacaaacgccc                                                                                                                     >  6:313949‑M1/1‑65 (MQ=255)                                                                                            gggCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTg                                                      <  4:143642/148‑1 (MQ=255)                                                                                                           aCCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCt                                      >  6:328463/1‑149 (MQ=255)                                                                                                               tGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTgg                                                     >  1:258936/1‑130 (MQ=255)                                                                                                               tGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTgg                                                     <  2:258936/130‑1 (MQ=255)                                                                                                                 gCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGc                                 <  5:285060/148‑1 (MQ=255)                                                                                                                  cTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTggcggc                                                                 >  2:193929/1‑115 (MQ=255)                                                                                                                  cTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTggcgg                                                                  >  1:115190/1‑114 (MQ=255)                                                                                                                  cTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTggcgg                                                                  <  2:115190/114‑1 (MQ=255)                                                                                                                       gCGTTGCTCACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGt                           <  3:258107/148‑1 (MQ=255)                                                                                                                       gCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGtt                          <  1:312169/149‑1 (MQ=255)                                                                                                                       gCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGtt                          >  1:25594/1‑149 (MQ=255)                                                                                                                         gTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGc                        <  4:160518/149‑1 (MQ=255)                                                                                                                            tctcaccggTGGCGTGCTGGGGCGTTTTTTTGCCGTGGCGCTTTATaacccccacgcacgccagcggtgtgcaacgccaaagccagggtaccggcattcggccccagcccagccgccagcacagtcagcgctgcccacaccagttcag                      >  6:323405‑M2/4‑46 (MQ=255)                                                                                                                            gCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCt                       <  5:315437/147‑1 (MQ=255)                                                                                                                            gCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCt                       >  6:315437/1‑147 (MQ=255)                                                                                                                                acCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGaacgccccagcacgccagcggtgtgcaacgccaaagccagggtaccggcattcggccccagcccagccgccagcacagtcagcgctgcccacaacagttcag                      <  5:323405‑M2/144‑103 (MQ=255)                                                                                                                                acCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTa                 <  2:170088/149‑1 (MQ=255)                                                                                                                                  cGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTa                 >  1:19722/1‑147 (MQ=255)                                                                                                                                     tGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTACAGCt            >  2:220567/1‑149 (MQ=255)                                                                                                                                               gggCGTTTGTTTGCCGAGGCGCTTGAGaacgccccagcacgccagcggtgtgcaacgccaaagccagggtaccggcattcggccccagccca                                                           >  6:313949‑M2/1‑27 (MQ=255)                                                                                                                                               gggCGTTTGTTTGCCGAGGCGCTTGAGaacgccccagcacgccagcggtgtgcaacgccaaagccagggtaccggcattcggccccagccca                                                           <  5:313949‑M2/92‑66 (MQ=255)                                                                                                                                               gggCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTACAGCTTGTACCGCTg  <  5:328463/149‑1 (MQ=255)                                                                                                                                               gggCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGGTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTACAGCTTGTACCGCTg  <  5:225881/149‑1 (MQ=255)                                                                                                                                               gggCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGAAGGGGGGCAGCCAGTTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACATGTGGCCGAAGTTGCTGGACTACAGCATGAACCGcag  >  4:47021/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                               TTGCAAGGGGCCGCGCGTTTGCTGCTCAATGCCTTGCGTGCCATTCCTGAACTGGTGTGGGC‑AGCGCTGACTGTGCTGGCGGCTGGGCTGGGGCCGAATGCCGGTACCCTGGCTTTGGCGTTGCACACCGCTGGCGTGCTGGGGCGTTTGTTTGCCGAGGCGCTTGAGAACGCCCCGCCCGAGCCGGCGGCGGCGATCCGTTTGCAGGGGGGCAGCCAGGTGGCGGCGTTCTGCTTTGGTACCCTGCCCAACCTGTGGCCGCAGTTGCTGGCCTACAGCTTGTACCGCTG  >  NC_002947/965307‑965596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.