New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	2460351 =	47 (0.900)	5 (0.100)	4/250	NT	10.7%	coding (453/480 nt)	PP_2153	hypothetical protein
	?	NC_002947	2460402 =	39 (0.800)					coding (402/480 nt)	PP_2153	hypothetical protein

CGCCATGGGACAGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAG  >  NC_002947/2460205‑2460497                                                                                                                                                   |                                                                                                                                                   cGCCATGGGACAGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAgctg                                                                                                                                                  <  1:114615/149‑1 (MQ=255)    cATGGGACAGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGctgctg                                                                                                                                               <  3:234212/149‑1 (MQ=255)    cATGGGACAGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGctgctg                                                                                                                                               >  2:258891/1‑149 (MQ=255)      cgggtgaaagtgaaaatggtgctgatgccgcgcgcccacggcctgctgctgcgcggcaaggtcacccattgcgaccctcgcccggagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGgc                                                                                                                                             <  7:95908‑M1/8‑1 (MQ=255)            atgCATTGCTGAAAGCTCTTCAGGGGGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCgtt                                                                                                                                       <  3:4425/147‑1 (MQ=255)            aGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCgtt                                                                                                                                       <  8:299252/149‑1 (MQ=255)                aTTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCgttgttg                                                                                                                                   <  1:311809/149‑1 (MQ=255)                    cTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGTCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGttgttgttg                                                                                                                                <  1:279099/148‑1 (MQ=255)                      gAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTg                                                                                                                             >  8:95908/1‑149 (MQ=255)                       aaaGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCt                                                                                                                              <  2:92262/147‑1 (MQ=255)                          gCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGgcgc                                                                                                                         <  2:251545/149‑1 (MQ=255)                           ctctTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCt                                                                                                                        >  6:5415/1‑149 (MQ=255)                                 aGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGTGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAgta                                                                                                                  <  7:84360/149‑3 (MQ=255)                                        tctcGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTAGCg                                                                                                           >  6:224259/1‑149 (MQ=255)                                         ccacggcctgctgctgcgcggcaaggtcacccattgcgaccctcgcccggagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCgg                                                                                                          <  4:143360‑M1/43‑1 (MQ=255)                                            gggTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCgg                                                                                                          <  7:18639/146‑1 (MQ=255)                                               tGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCgttgttgt                                                                                                                                  >  2:308185/1‑119 (MQ=255)                                               tGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCgttgttgt                                                                                                                                  <  1:308185/119‑1 (MQ=255)                                                  ttCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGt                                                                                                 >  2:141912/1‑149 (MQ=255)                                                   ctgctgcgcggcaaggtcacccattgcgaccctcgcccggagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagcag                                                                                                  >  3:143360‑M1/97‑147 (MQ=255)                                                      ctgcgcggcaaggtcacccattgcgaccctcgcccgaagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTg                                                                                               >  5:57445‑M1/94‑147 (MQ=255)                                                           ggCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTg                                                                                         <  5:224259/148‑1 (MQ=255)                                                            gcgcCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTggg                                                                                       >  6:144948/1‑149 (MQ=255)                                                               ccgcGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGa                                                                                                                  >  5:201863/1‑119 (MQ=255)                                                               ccgcGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGa                                                                                                                  <  6:201863/119‑1 (MQ=255)                                                                  cgATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGa                                                                                                                  <  1:26793/116‑1 (MQ=255)                                                                  cgATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGa                                                                                                                  >  2:26793/1‑116 (MQ=255)                                                                        ccGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTAGTTCAGTTCCAGCTGCTGTCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCATTTGTCGTTGGGCGTCGGTCATGt                                                                           >  8:126147/1‑149 (MQ=255)                                                                          gTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTc                                                                          >  6:77084/1‑148 (MQ=255)                                                                             gATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGAt                                                                       <  1:141912/148‑1 (MQ=255)                                                                              gaccctcgcccggagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagcag                                                                                                  >  6:79537‑M1/70‑120 (MQ=255)                                                                              gaccctcgcccggagggcggcttcgaggtcggtaccgagttcatcgacatgaccgacgcccaacgacaaCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagcag                                                                                                  <  5:79537‑M1/51‑1 (MQ=255)                                                                               tCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagca                                                                                                   >  2:175047/1‑118 (MQ=255)                                                                               tCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagca                                                                                                   <  1:175047/118‑1 (MQ=255)                                                                                         gttcgttcCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTAcc                                                           >  3:306092/1‑148 (MQ=255)                                                                                          ttcgttcCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGa                                                         >  7:234447/1‑149 (MQ=255)                                                                                              ttcCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGGTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGGCAGCAGTTGTCGTTCGGCGTCGGTCATGTCTATGACATCGGTACCGACCTc                                                     >  8:109985/1‑149 (MQ=255)                                                                                                 cTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagcag                                                                                                  <  4:303899/101‑1 (MQ=255)                                                                                                  ttCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAg                                                 >  7:136883/1‑149 (MQ=255)                                                                                                   tCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGaa                                                  >  8:161603/1‑147 (MQ=255)                                                                                                   tCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGaa                                                  <  7:157144/147‑1 (MQ=255)                                                                                                   tCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGaa                                                  <  7:161603/147‑1 (MQ=255)                                                                                                   tCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGaa                                                  >  8:157144/1‑147 (MQ=255)                                                                                                     aaaTCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAgccg                                              <  5:144948/149‑1 (MQ=255)                                                                                                            ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAGCTCGGTACCGACCTCGAAGCCGCCCTCCg                                       >  3:37407/1‑149 (MQ=255)                                                                                                             gAAAAATTGGCCCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTCCCGCCCGCGAAGCCGCCCTCCgg                                      >  2:227196/1‑149 (MQ=255)                                                                                                               aaaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCggg                                     <  4:306092/148‑1 (MQ=255)                                                                                                                        tCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCg                            >  5:134581/1‑148 (MQ=255)                                                                                                                           ggaggCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAAt                        <  8:136883/149‑1 (MQ=255)                                                                                                                                    gTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCt               >  6:15458/1‑149 (MQ=255)                                                                                                                                     tCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGCCCtt              >  8:25702/1‑149 (MQ=255)                                                                                                                                       gTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGc            <  6:134581/149‑1 (MQ=255)                                                                                                                                         tCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCcg          >  2:241477/1‑149 (MQ=255)                                                                                                                                             ttCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCgca      <  7:126147/149‑1 (MQ=255)                                                                                                                                               ccAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCgcag     >  5:303640/1‑148 (MQ=255)                                                                                                                                                 aGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGGCGCAATGGGTGACCTTGcc           <  4:246859/140‑1 (MQ=255)                                                                                                                                                  gctgctGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCgcagcag  >  7:16924/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGCCATGGGACAGGCATTGCTGAAAGCTCTTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAG  >  NC_002947/2460205‑2460497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.