New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 6002866	49 (0.950)	4 (0.080)	4/240	NT	9.3%	coding (1071/1491 nt)	amaB	L‑piperidine‑6‑carboxylate dehydrogenase
	?	NC_002947	= 6002960	33 (0.690)					coding (977/1491 nt)	amaB	L‑piperidine‑6‑carboxylate dehydrogenase

GCAGGGCCTCCTCGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCACTTTGCCGTAGG  >  NC_002947/6002730‑6003011                                                                                                                                         |                                                                                                                                                    gcAGGGCCTCCTCGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCg                                                                                                                                         >  7:86497/1‑149 (MQ=255)   aGGGCCTCCTCGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGc                                                                                                                                        >  7:284166/1‑148 (MQ=255)         tcctcGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGaa                                                                                                                                 >  5:261641/1‑149 (MQ=255)         tcctcGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGaa                                                                                                                                 >  3:23309/1‑149 (MQ=255)            tcGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATAc                                                                                                                              >  7:178052/1‑149 (MQ=255)                     tcgaAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCAcc                                                                                                                     >  5:21449/1‑149 (MQ=255)                       gtAGGCCATCACCTACACGAAACGAACCAACGTTTCGTGGCGCACCACGTCACTTTGCGAAGGCATATCAGCAATCGCTCGCGAAACGTAGAAGCCGTTCGCGTACTGGTCGGCCATCTGAAGCTCCCCAACGAATTCCTGGCCAcagt                                                                                                                   >  8:298166/1‑146 (MQ=255)                          ggCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑tcgt                                                                                                               >  4:290799/1‑149 (MQ=255)                           gCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑tcgtc                                                                                                              <  4:133511/149‑1 (MQ=255)                            ccAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑tcgtcg                                                                                                             >  5:186276/1‑149 (MQ=255)                            ccAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑tcgtcg                                                                                                             <  6:261641/149‑1 (MQ=255)                             cAGCACGTACTGGTTCGTTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTcgc                                                                                                            <  8:284166/149‑1 (MQ=255)                                   gTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCt                                                                                                     >  1:290926/1‑149 (MQ=255)                                       aGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGc                                                                                                 >  6:105889/1‑149 (MQ=255)                                              gTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTgcg                                                                                          >  2:133617/1‑149 (MQ=255)                                               tGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCTTCGC‑GGGCCTTGGCCAGTgcgc                                                                                         >  2:209237/1‑149 (MQ=255)                                                 cGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGccc                                                                                       <  3:290799/149‑1 (MQ=255)                                                   gaGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTg                                                                                                                           >  7:83172/2‑113 (MQ=255)                                                   gaGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTg                                                                                                                           <  8:83172/112‑1 (MQ=255)                                                         gcGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGa                                                                                                                                               <  6:224260/86‑1 (MQ=255)                                                           gTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGt                                                                             >  2:318576/1‑149 (MQ=255)                                                            tGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCACCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTc                                                                            <  2:277195/149‑1 (MQ=255)                                                                gcACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCcgctgatcgacaagcagtcgttcgacgccatgcagggcgcactggccaaggcccgcgacgaaggtggccaggtat                                                                          >  6:168642‑M1/1‑74 (MQ=255)                                                                 caccacGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACg                                                                       <  2:93886/149‑1 (MQ=255)                                                                   ccacGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGAc                                                                     <  4:23309/149‑1 (MQ=255)                                                                      cGTCACTTTGCGCGGGCATTTGAGCAATGGCTGGCGAAAGGTAGTAGGCGTTCGGGTACTGGTCGGGCAGCTGACGCTCGCCACCGTATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGc                                                                  <  3:164776/149‑1 (MQ=255)                                                                      cGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGTCAGTGCGCCCTGCATGGCGTCGAACGACTGc                                                                  >  6:156142/1‑149 (MQ=255)                                                                      cGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGTCAGTGCGCCCTGCATGGCGTCGAACGACTGc                                                                  <  5:156142/149‑1 (MQ=255)                                                                        tCACTTTGCGCCGGCATTTCAGCAATGTCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCtt                                                                <  8:288224/149‑1 (MQ=255)                                                                               gcgcCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGAt                                                          <  1:209237/148‑1 (MQ=255)                                                                               gcgcCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGAt                                                          <  1:318576/148‑1 (MQ=255)                                                                                 gcCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAg                                                       >  4:203674/1‑149 (MQ=255)                                                                                       aTTTCTGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCagctgatcgacaagcagtagttcgactccatgcagggcgcactgtccaaggccctcgacgaaggtggccaggtattcgttggcgagcttcagctggcc                                                   <  4:39675‑M1/149‑99 (MQ=255)                                                                                       aTTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTg                                                                                            <  8:75524/106‑1 (MQ=255)                                                                                       aTTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTg                                                                                            >  7:75524/1‑106 (MQ=255)                                                                                           cAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCcgctgatcgacaagcagtcgttcgacgccatgcagggcgcactggccaaggcccgcgacgaaggtggccaggtattcggtggcgagcgtcagctggccgac                                                <  5:168642‑M1/148‑102 (MQ=255)                                                                                            aGCAATGGCTGGCGAAACGTAGTAGGCGTTCGCGTACTGGTCGGCCcgctgatcgacaagcagtcgttcgacgccatgcagggcgcactggccaaggcccgcgacgaaggtggccaggtattcggtggcgagcgtcagctggccgacc                                               <  2:141281‑M1/148‑103 (MQ=255)                                                                                             gCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGggccgaccag                                           >  8:178052‑M2/1‑139 (MQ=255)                                                                                                    ctggcGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCt                                    >  8:258393/1‑149 (MQ=255)                                                                                                          aaaCGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGt                              <  1:133617/149‑1 (MQ=255)                                                                                                           aaCGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGCAAGGCCTTGGCCAGTGCCCCCTGAATGGCGTCGAACGACTGCTTTACGATCCGCGGCCCGACCTGGTTGTATTTACGt                              >  1:266826/1‑149 (MQ=255)                                                                                                                agtagGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCTGACGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTc                         >  1:94748/1‑149 (MQ=255)                                                                                                                      cGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCTAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATg                  <  6:186276/149‑1 (MQ=255)                                                                                                                       gTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTc                                                                            >  7:209280/1‑90 (MQ=255)                                                                                                                       gTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTc                                                                            <  8:209280/90‑1 (MQ=255)                                                                                                                       gTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTg                             <  1:260482/137‑1 (MQ=255)                                                                                                                       gTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTg                             >  2:260482/1‑137 (MQ=255)                                                                                                                          cGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATgcgc               <  8:86497/148‑1 (MQ=255)                                                                                                                           gggTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCa              <  4:303315/148‑1 (MQ=255)                                                                                                                           gggTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCa              >  3:303315/1‑148 (MQ=255)                                                                                                                           gggTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCa              <  1:50574/148‑1 (MQ=255)                                                                                                                                cTGGTCGGCCcgctgatcgacaagcagtcgttcgacgccatgcagggcgcactggccaaggcccgcgacgaaggtggccaggtattcggtggcgagcgtcagctggccgaccagtacccgaacgcctactacgtttcgccagccattgc          >  8:178052‑M1/1‑10 (MQ=255)                                                                                                                                cTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCACTTTGc        <  6:21449/149‑1 (MQ=255)                                                                                                                                  ggTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGggccgaccagtacgcgaacgcctactacgtttcgccagccattgct       <  2:141281‑M2/148‑47 (MQ=255)                                                                                                                                  ggTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCACTTTGCCg      >  8:153201/1‑149 (MQ=255)                                                                                                                                   gTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGggccgaccagtacccgaacgcctactacgtttcgccagccattgctg      <  5:168642‑M2/148‑48 (MQ=255)                                                                                                                                    tCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGGGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCACTTTGCCGTa    <  7:298166/149‑1 (MQ=255)                                                                                                                                      ggCCAGCTGAAGCTCGCCAACGAATACCTGGCCACCT‑TCGTCGA‑GGGCCTTGGACAGTGCGCCCTGCATGGAGTCGAACTACTGCTTGTCGATCAGCTggccgaccagtacccgaacgcctactacgtttcgccagccattgcagaaat  <  4:39675‑M2/149‑52 (MQ=255)                                                                                                                                         |                                                                                                                                                    GCAGGGCCTCCTCGAAGTCGTCGTAGGCCAGCACGTACAGGATCGGTGCGAAGGTTTCGTGGCGCACCACGTCACTTTGCGCCGGCATTTCAGCAATGGCTGGCGAAACGTAGTAGGCGTTCGGGTACTGGTCGGCCAGCTGACGCTCGCCACCGAATACCTGGCCACCT‑TCGTCGC‑GGGCCTTGGCCAGTGCGCCCTGCATGGCGTCGAACGACTGCTTGTCGATCAGCGGGCCGACCAGGTTGTCTTTACGTGGGTCGCCGATGCGCACTTTGCCGTAGG  >  NC_002947/6002730‑6003011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.