New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 765795	39 (0.690)	3 (0.050)	3/250	NT	6.5%	coding (390/936 nt)	PP_0658	homocysteine S‑methyltransferase family protein
	?	NC_002947	= 765837	49 (0.890)					coding (432/936 nt)	PP_0658	homocysteine S‑methyltransferase family protein

GTGAAGAACGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGTTCTGGGTG  >  NC_002947/765647‑765942                                                                                                                                                     |                                                                                                                                                     gTGAAGAACGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGCCCTGTTCCAG                                                                                                                                                      >  4:100053‑M1/1‑149 (MQ=255) gTGAAGAACGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAG                                                                                                                                                      <  3:250713‑M1/149‑1 (MQ=255)    aagaaCGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGccc                                                                                                                                                   >  4:195429/1‑149 (MQ=255)      gaaCGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGccc                                                                                                                                                   >  6:207525/1‑147 (MQ=255)        aCGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGc                                                                                                                                               <  7:175337/149‑1 (MQ=255)            tttGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCgtgt                                                                                                                                           >  1:265498/1‑149 (MQ=255)              tGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCgtg                                                                                                                                            <  8:254525/146‑1 (MQ=255)              tGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCgtg                                                                                                                                            >  7:254525/1‑146 (MQ=255)                ccgccgAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTgg                                                                                                                                         >  5:111103/1‑147 (MQ=255)                 cgccgAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGCGCGTGTgg                                                                                                                                         >  4:238333/1‑146 (MQ=255)                        gggCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGGGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACGTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCt                                                                                                                               <  7:84558/149‑1 (MQ=255)                                        atatCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAg                                                                                                               <  8:77638/149‑1 (MQ=255)                                        atatCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAg                                                                                                               >  5:307154/1‑149 (MQ=255)                                            cGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCc                                                                                                           <  2:314648‑M2/149‑2 (MQ=255)                                             gCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGTGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCc                                                                                                           >  6:31222‑M2/1‑147 (MQ=255)                                             gCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGtgtg                                                                                                                                          <  6:258630/117‑1 (MQ=255)                                             gCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGtgtg                                                                                                                                          >  5:258630/1‑117 (MQ=255)                                               aGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCctgg                                                                                                        <  4:8113‑M2/149‑5 (MQ=255)                                                   cAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGcccc                                                                                                    <  3:238333/149‑1 (MQ=255)                                                    aGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGgccctggagcaacggggtcagcacctctgccacacgctcgggctggaacag                                                                                                   <  7:94056‑M1/149‑52 (MQ=255)                                                          cgcggcacgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGt‑t                                                                                            <  2:247579/149‑1 (MQ=255)                                                          cgcggcacgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGt                                                                                              <  6:307154/148‑1 (MQ=255)                                                           gcggcacgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑Tg                                                                                           >  6:42514/1‑149 (MQ=255)                                                             ggcacgcggcaGATAGCGGCGCGCTCCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGAc                                                                                         <  4:287728/149‑1 (MQ=255)                                                             ggcacgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGAc                                                                                         <  6:212045/149‑1 (MQ=255)                                                             ggcacgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGAc                                                                                         <  8:16734/149‑1 (MQ=255)                                                                acgcggcaGATAGCGGCGCGCACCCGGTCCGGGTGGCAGGGGCGCGGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCCGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCtg                                                                                      <  3:90126/149‑1 (MQ=255)                                                                acgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCtg                                                                                      <  3:90123/149‑1 (MQ=255)                                                                 cgcggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCtgt                                                                                     <  3:8113/149‑1 (MQ=255)                                                                    ggcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtggc                                                                                  >  3:215294/1‑149 (MQ=255)                                                                     gcaGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtggct                                                                                 <  1:314648/149‑1 (MQ=255)                                                                               gcgcgcACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtgg                                                                                   >  3:297976/1‑137 (MQ=255)                                                                               gcgcgcACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtgg                                                                                   <  4:297976/137‑1 (MQ=255)                                                                               gcgcgcACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGGGGCTGGCCGAAAcc                                                                       <  6:111103/149‑1 (MQ=255)                                                                                        cGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTcc                                                              <  7:28311/149‑1 (MQ=255)                                                                                        cGGGCAGCGTGGCAGGGTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTGCCAGCCCGAGCGTGGGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTcc                                                              <  2:265498/149‑1 (MQ=255)                                                                                                 tGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGgccctggagcaacggggtcagcacctctgccacacgctcgggctggaaca                                                                                                    <  7:17654‑M1/103‑51 (MQ=255)                                                                                                 tGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGgccctggagcaacggggtcagcacctctgccacacgctcgggctggaaca                                                                                                    >  8:17654‑M1/1‑53 (MQ=255)                                                                                                 tGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCg                                                       >  5:35405/1‑147 (MQ=255)                                                                                                                    gcTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGc                                   >  1:182692/1‑148 (MQ=255)                                                                                                                    gcTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGc                                   >  6:341911/1‑148 (MQ=255)                                                                                                                           gggTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGcc                           <  6:35405/149‑1 (MQ=255)                                                                                                                                  aCCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGAc                    >  6:123897/1‑149 (MQ=255)                                                                                                                                         cGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGc             <  5:341911/149‑1 (MQ=255)                                                                                                                                             cTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgccaccctgaccgggtgcgcgccgctatctgccgcgtgccgcgcaagct          <  7:94056‑M2/149‑99 (MQ=255)                                                                                                                                             cTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtggctggc                                                                              >  6:101935/1‑80 (MQ=255)                                                                                                                                             cTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGtggctggc                                                                              <  5:101935/80‑1 (MQ=255)                                                                                                                                             cTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGtt         >  2:205432/1‑149 (MQ=255)                                                                                                                                              tGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgcca                                                       >  8:17654‑M2/1‑50 (MQ=255)                                                                                                                                              tGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgcca                                                       <  7:17654‑M2/103‑54 (MQ=255)                                                                                                                                                ttCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGTTc        <  4:215294/147‑1 (MQ=255)                                                                                                                                                ttCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGTTCTg      >  1:199946/1‑149 (MQ=255)                                                                                                                                                  ccAGgccctggagcaacggggtcagcacctctgccacacgctcgggctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgccaccctgaccgggtgcgcgccgctatctgccgcgtgccgcgcaagctggcct     <  4:8113‑M1/149‑146 (MQ=255)                                                                                                                                                  ccAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGTTCTgg     <  5:207525/148‑1 (MQ=255)                                                                                                                                                     ggccctggagcaacggggtcagcacctctgccacacgctcgggctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgccaccctgaccgggtgcgcgccgctatctgccgcgtgccgcgcaagctggcctgcg  <  2:314648‑M1/149‑149 (MQ=255)                                                                                                                                                     ggccctggagcaacggggtcagcacctctgccacacactcgggctggaacaggtcgggccggtaggacccgaacagcggcggcagcgaacctgccaccctgaccgggtgcgcgccgctatctgccgcgtgccgcgcaagctggcctgc   >  6:31222‑M1/1‑1 (MQ=255)                                                                                                                                                     gCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGTGATGCCTGTGGCTGGCCCAAACCCACAGGTACCTTGCCGAAGTGCGGGCCATTAAAAACCATTTTCCCGCAAAAGGCCAGCCGttaatggtt  >  1:98978/1‑142 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTGAAGAACGCTTTGCCGCCGAAGGGCGACAGCTGGCCCATATCGCAGGCCAGCTTGCGCGGCACGCGGCAGATAGCGGCGCGCACCCGGTCAGGGTGGCAGGTTCGCTGCCGCCGCTGTTCGGGTCCTACCGGCCCGACCTGTTCCAGCCCGAGCGTGTGGCAGAGGTGCTGACCCCGTTGCTCCAGGGCCTGGCCCCGCATGT‑TGACCTGTGGCTGGCCGAAACCCAGAGTTCCGTGGCCGAAGTGCGAGCCATTCATAGCCATTTGCCGGCAGACGGCAGGCCGTTCTGGGTG  >  NC_002947/765647‑765942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.