New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_002947 | 2982409 = | 50 (0.880) | 3 (0.060) | 3/246 | NT | 5.8% | coding (770/810 nt) | PP_2608 | shikimate dehydrogenase family protein |
? | NC_002947 | 2982442 = | 49 (0.910) | coding (737/810 nt) | PP_2608 | shikimate dehydrogenase family protein |
CCACGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGGGCGGCG > NC_002947/2982267‑2982555 | gcatgggcacccgcgcagagctgccgttgtccgccgccctgctggctaccctgcagccagacaccctggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGc > 4:283077‑M1/146‑149 (MQ=255) cACGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTAAGATCTCCAACCGTGTGACGCCCAAGAAGGCGCCAAGGTGGCCCac > 6:109349/1‑148 (MQ=255) cACGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGCGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAg > 5:355163/1‑149 (MQ=255) cACGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGGTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCGCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAg > 3:85972/1‑149 (MQ=255) aCGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGCA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAACGTGGCCCAGt > 5:6931/1‑149 (MQ=255) gcggGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTgg < 4:75589/149‑1 (MQ=255) cgcgGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAg < 2:136662/149‑1 (MQ=255) cgcgGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGGCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGGGCCAAGGTGGCCCAGTTGGGCGAAg < 5:109349/149‑1 (MQ=255) tGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCAt < 4:236454/149‑1 (MQ=255) gcagcggcaAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGCGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCg < 6:355163/149‑1 (MQ=255) gagcgggaAAAACAACGGGACCGCTTCCGAGGATAATCATGTCGAATTTACCGTTGTGTGTGTGCTCCAGACGCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTc < 1:115987/148‑1 (MQ=255) gcaAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAg > 2:13428/1‑149 (MQ=255) cgccgccctgctggctaccctgcagccagacaccccggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGcc < 6:143901‑M1/5‑1 (MQ=255) cgccgccctgctggctaccctgcagccagacaccccggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGcc > 5:143901‑M1/115‑119 (MQ=255) gccgccctgctggctaccctgcagccagacaccctggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTc > 5:244206‑M1/114‑149 (MQ=255) caccGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGAt < 6:159795/149‑1 (MQ=255) accGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGCGCGAAGGCCATTTCCGGGCCAGTCTGGATc < 6:6931/149‑1 (MQ=255) ggCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTg < 1:13428/149‑1 (MQ=255) aCCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCaa > 4:267760/1‑149 (MQ=255) ccGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGCCGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAAc < 6:58398/149‑1 (MQ=255) tGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑Ac > 4:132313/1‑149 (MQ=255) agacaccctggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCa > 4:331900‑M1/88‑120 (MQ=255) agacaccctggtcgccgacgtggtcacctcacccgagatcaccccgctgctgaaccgcgcgcggcaagtaggttgcaggatccagacTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCa < 3:331900‑M1/33‑1 (MQ=255) gAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTcag > 6:11340/1‑148 (MQ=255) cTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCg > 7:68352/1‑149 (MQ=255) cTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCg > 7:73232/1‑149 (MQ=255) cTGTTGTGTGTGTGCTCCAGAGGCGTCATGGCTTCACATCTCCAACGGTGTGACGCCCATGCAGGCGCCAAGGGGGCCCAGTTGGGCGAAGGCCATTTCCGGCCCAGTCTGGATCCTGCAACCTAACTTGCCGCGCGCGGTTCagcagc < 7:225125/149‑1 (MQ=255) tgttgtGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCgg > 1:867/1‑149 (MQ=255) ttgtGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCggg < 3:267760/148‑1 (MQ=255) ttgtGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCggg < 7:97594/148‑1 (MQ=255) ttgtGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCggg < 5:11340/148‑1 (MQ=255) tgtgtgtgCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTg < 3:157632/148‑1 (MQ=255) tgtgtgtgCTCCAGAGTCGTCATGGCTTCAGAGCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTg < 4:85972/148‑1 (MQ=255) tgtgtgCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGAtc > 8:278730/1‑149 (MQ=255) tCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGggt > 7:245988/1‑148 (MQ=255) cATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACg > 8:67174/1‑149 (MQ=255) ggCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCg > 1:330640/1‑149 (MQ=255) ttCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGggtgag < 3:154078/134‑1 (MQ=255) ttCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGggtgag > 4:154078/1‑134 (MQ=255) ttCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCg > 2:36644/1‑149 (MQ=255) cAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGa < 7:67174/148‑1 (MQ=255) cAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGa < 7:278730/148‑1 (MQ=255) cAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGGGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGa < 2:136562/148‑1 (MQ=255) aaCGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTc < 3:132313/149‑1 (MQ=255) cGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTg > 1:302119/1‑149 (MQ=255) ggTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTgg < 8:12721/149‑1 (MQ=255) ggTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTgg > 7:12721/1‑149 (MQ=255) ggTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTgg < 2:330640/149‑1 (MQ=255) gCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAggg > 5:56475/1‑149 (MQ=255) ccATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAggg > 1:36032/1‑147 (MQ=255) cATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAggg > 6:247165/1‑146 (MQ=255) cATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTa < 5:247165/148‑1 (MQ=255) ggCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAgg < 1:266359/124‑1 (MQ=255) ggCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAgg > 2:266359/1‑124 (MQ=255) gcCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTcagc > 6:275192/1‑79 (MQ=255) gcCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTcagc < 5:275192/79‑1 (MQ=255) cAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGggcg < 8:68352/149‑1 (MQ=255) cAAGGTGGCCCAGTGGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGggcg < 8:73232/149‑1 (MQ=255) ggTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGGgcggcg < 2:867/149‑1 (MQ=255) gTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGGgcggcg > 6:38074/1‑148 (MQ=255) | CCACGGCGGGCCGCGGTGGCGGCAGCGGCAAAACCACCGGCACCGCTGCCCAGCACAACCAGGTCGAA‑‑‑GCTGTTGTGTGTGTGCTCCAGAGTCGTCATGGCTTCAGATCTCCAACGGTGTGACGCCCATGAAGGCGCCAAGGTGGCCCAGTTGGGCGAAGGCCATTTCCGGGCCAGTCTGGATCCTGCAACCT‑ACTTGCCGCGCGCGGTTCAGCAGCGGGGTGATCTCGGGTGAGGTGACCACGTCGGCGACCAGGGTGTCTGGCTGCAGGGTAGCCAGCAGGGCGGCG > NC_002947/2982267‑2982555 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |