New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 4117855	43 (0.760)	5 (0.090)	5/244	NT	11.3%	coding (545/2256 nt)	PP_3622	isoquinoline 1‑oxidoreductase subunit beta
	?	NC_002947	= 4117881	38 (0.710)					coding (571/2256 nt)	PP_3622	isoquinoline 1‑oxidoreductase subunit beta

GCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACAAGTCCACCGG  >  NC_002947/4117720‑4118002                                                                                                                                          |                                                                                                                                                      gcATGAGCTACCCCACCATGCGCCGTCTGGGCGCCCTCGCCCGCGGCATGCAGCTGCAGGCGGGCGCCGAACACCTCGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCAGTAccc                                                                                                                                          >  4:232470/1‑149 (MQ=255)   aTGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTa                                                                                                                                        >  1:289566/1‑149 (MQ=255)           ccccACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGc                                                                                                                               >  8:251571/1‑149 (MQ=255)            cccACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGccagctcaccgtagggtacggaac                                                                                                                               >  2:266808‑M1/1‑125 (MQ=255)              caccaTGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACg                                                                                                                                      >  3:353300/1‑140 (MQ=255)              caccaTGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACg                                                                                                                                      <  4:353300/140‑1 (MQ=255)              caccaTGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTgg                                                                                                                            >  4:246709/1‑149 (MQ=255)               aCCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGc                                                                                                                           <  2:111322‑M2/149‑2 (MQ=255)                 cATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGccg                                                                                                                         >  5:364264‑M2/1‑146 (MQ=255)                     cgcCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGccagctcaccgtagggtacggaacgtccggtcg                                                                                                                      <  1:70665‑M1/149‑34 (MQ=255)                      gcCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑Tcgc                                                                                                                   <  7:251571/149‑1 (MQ=255)                         gTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGcc                                                                                                                <  6:98828/149‑1 (MQ=255)                          tCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTAcc                                                                                                                                           >  4:84788/1‑123 (MQ=255)                          tCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTAcc                                                                                                                                           <  3:84788/123‑1 (MQ=255)                             gggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACCTACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATgg                                                                                                            >  4:300576/1‑149 (MQ=255)                             gggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATg                                                                                                             >  4:295636/1‑148 (MQ=255)                              ggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGa                                                                                                           >  6:33075/1‑149 (MQ=255)                              ggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGa                                                                                                           >  3:3007/1‑149 (MQ=255)                               gcgcgcTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGccagctcaccgtagggtacggaacgtccggtcgtggtatgtac                                                                                                            <  1:266808‑M1/149‑44 (MQ=255)                               gcgcgcTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGAc                                                                                                          >  4:16204/1‑149 (MQ=255)                                   gcTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGc                                                                                                      <  5:33075/149‑1 (MQ=255)                                     tCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCg                                                                                                    >  4:223512/1‑149 (MQ=255)                                     tCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCAAGGACCTGCCg                                                                                                    >  7:245514/1‑149 (MQ=255)                                       gCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGt                                                                                                  >  7:219020/1‑149 (MQ=255)                                           ggggCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGccc                                                                                              <  6:348588/149‑1 (MQ=255)                                                  gctgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGccagctcaccgtagggtacggaacgtccggtcgt                                                                                                                     >  6:226000‑M1/1‑87 (MQ=255)                                                  gctgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGccagctcaccgtagggtacggaacgtccggtcgt                                                                                                                     <  5:226000‑M1/121‑35 (MQ=255)                                                   ctgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGc                                                                                      <  3:300576/149‑1 (MQ=255)                                                   ctgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGc                                                                                      <  3:295636/149‑1 (MQ=255)                                                              gggcgCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGc                                                                           >  5:341713/1‑149 (MQ=255)                                                                 cgcCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTgc                                                                        <  6:91707/149‑1 (MQ=255)                                                                   ccGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACACGC‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGCCCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTgcg                                                                       >  1:250719/1‑149 (MQ=255)                                                                    cGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑CGGGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTgcgc                                                                      >  1:250474/1‑149 (MQ=255)                                                                              ggTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGCCCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCccagcca                                                           <  2:250719/149‑1 (MQ=255)                                                                              ggTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCccagcca                                                           <  3:223512/149‑1 (MQ=255)                                                                              ggTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCccagc                                                             <  4:3007/147‑1 (MQ=255)                                                                               gtgtACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCa                                                                                                               >  1:284201/1‑96 (MQ=255)                                                                               gtgtACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCa                                                                                                               <  2:284201/96‑1 (MQ=255)                                                                                       gTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTg                                                  <  8:219020/149‑1 (MQ=255)                                                                                       gTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTg                                                  <  3:16204/149‑1 (MQ=255)                                                                                            tGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGAtt                                              <  8:110078/148‑1 (MQ=255)                                                                                            tGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGAtt                                              >  7:110078/1‑148 (MQ=255)                                                                                             gAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACAACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTg                                             >  2:52189/1‑148 (MQ=255)                                                                                              atcTTTCTACCACAA‑C‑GGGTAACGTACTAAAAACCACGACCGGAAGTTTCGTACCCTACGG‑GGTTCTGGGCGGTTGGCTGCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTg                                             <  1:111322/146‑1 (MQ=255)                                                                                                                gggTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACgccgcc                           >  3:118675/1‑149 (MQ=255)                                                                                                                     aCGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGAt                      <  3:246709/149‑1 (MQ=255)                                                                                                                       gtagtaCATACCACGACCGGACGTTCCGTACCCTACGGTTGACATGGCCGG‑TCGCGCCATGGCCATCCCGTTGCCCGACCCGGCCAGCGTCAAGCTGCCCGACCCCAGCCAGTTTCGCTGGATTGGAAACCCCGTACGCCGCCTGGATg                     >  1:59057/1‑149 (MQ=255)                                                                                                                          gtaCATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagcatgccccgggcgagcgcgc                  <  1:266808‑M2/149‑107 (MQ=255)                                                                                                                                 ccACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACaa           <  1:52189/148‑1 (MQ=255)                                                                                                                                  cACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTAGAAAGCCCGTACGCCGCCTGGATGCCTACGACAAGt         >  6:144915/1‑149 (MQ=255)                                                                                                                                   aCGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagc                                     >  6:226000‑M2/1‑34 (MQ=255)                                                                                                                                   aCGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagc                                     <  5:226000‑M2/121‑88 (MQ=255)                                                                                                                                   aCGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCAGACCCGGCCAGCGTCAAGCTTCGCGACCCCCGCCACTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACAACTc        >  3:322698/1‑149 (MQ=255)                                                                                                                                    cGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagcatgccccgggcgagcgcgcccagacggcg        <  1:70665‑M2/149‑117 (MQ=255)                                                                                                                                        cGGccagctcaccgtagggtacggaacgtccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagcatgccccgggcgagcgcgcccagacggcgcatg     >  5:364264‑M1/1‑3 (MQ=255)                                                                                                                                        cGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACAAGTCCACCg   <  2:289566/149‑1 (MQ=255)                                                                                                                                         ggACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCGGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACAAGTCCACCgg  >  4:192538/1‑149 (MQ=255)                                                                                                                                          gccagctcaccgtagggtacggaacgtccggtcgtggtatgtactacgttacccggtgtggtagaaagctcatcgaccggtacacccaggtgttcggcgcccgcctgcagcagcatgccccgggcgagcgcgcccagacggcgcatggt   <  2:111322‑M1/149‑149 (MQ=255)                                                                                                                                          |                                                                                                                                                      GCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACAC‑C‑GGGTAACGTAGTACATACCACGACCGGACGTTCCGTACCCTACGG‑TGAGCTGGCCGG‑TCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTGGAAAGCCCGTACGCCGCCTGGATGCCTACGACAAGTCCACCGG  >  NC_002947/4117720‑4118002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.