New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	5299051 =	48 (0.910)	3 (0.060)	3/244	NT	5.9%	coding (1051/2076 nt)	recD	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease
	?	NC_002947	5299093 =	49 (0.970)					coding (1009/2076 nt)	recD	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease

ACCTGCTGAAGCCACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAGGCGATGCACGGTGCTGAC  >  NC_002947/5298948‑5299237                                                                                                                                                   |                                                                                                                                                 gcggcactttcgccatcatgctggcaacccgttgccgttggatgtgctggtggtcgacgaagcgtcgatgatcgacctggaaatgatggccaacctgctcgatgCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCa                                                                                                                                                 >  7:300098‑M1/105‑148 (MQ=255)   ctgctgAAGCCACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATc                                                                                                                                               >  3:135204/1‑147 (MQ=255)        gAAGCCACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAgg                                                                                                                                        >  3:125036/1‑149 (MQ=255)          aGCCACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGtt                                                                                                                                      <  4:135204/149‑1 (MQ=255)            ccACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTgg                                                                                                                                    >  4:83686/1‑149 (MQ=255)             cACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGc                                                                                                                                   <  5:57447/149‑1 (MQ=255)              aCGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGcc                                                                                                                                  <  3:83686/149‑1 (MQ=255)              aCGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGc                                                                                                                                   >  4:251215/1‑148 (MQ=255)                     aGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCAttt                                                                                                                           >  3:281203/1‑149 (MQ=255)                         gCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCa                                                                                                                        <  3:251215/148‑1 (MQ=255)                                aGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGatc                                                                                                                <  3:54323/149‑1 (MQ=255)                                 gTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGatca                                                                                                               >  1:55404/1‑149 (MQ=255)                                  tAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCcatca                                                                                                                              <  6:265853/133‑1 (MQ=255)                                  tAAAAGCCATCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCcatca                                                                                                                              >  5:265853/1‑133 (MQ=255)                                      aGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGAc                                                                                                          <  1:249925/149‑1 (MQ=255)                                              tcAGCATCCCGGCACAGGTCACCCAGCACCGCGCCCGCCTCGACCCAGGCCAACTGGAGCCTTGTCGCCCAGCAGCACCAGACCCGCCCGCGGCGGCAAGGCATCCAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACCCTtcgt                                                                                                   >  1:96829/1‑149 (MQ=255)                                               cAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCcatc                                                                                                                               <  5:157688/119‑1 (MQ=255)                                               cAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCcatc                                                                                                                               >  6:157688/1‑119 (MQ=255)                                                aGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTtcgtc                                                                                                  <  4:125036/147‑1 (MQ=255)                                                aGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGa                                                                                                >  6:220858/1‑149 (MQ=255)                                                    tCCCGGCTCAGGTCACGCAGCACCGCGCCGGCGTCGACCGAGGCCAAGTGG‑TCCTTGTCGCCCAGGAGCACCAGACGCGCCGGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATGATCGACGCTTCGTCGaccac                                                                                            <  2:96829/149‑1 (MQ=255)                                                         gCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTAGATCATCGACGCTTCGTCGACCACCAGc                                                                                        >  4:98120/1‑148 (MQ=255)                                                               gTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATc                                                                                   <  5:221089/147‑1 (MQ=255)                                                               gTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATc                                                                                   >  6:221089/1‑147 (MQ=255)                                                                     cAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacggc                                                                            >  7:168334/1‑148 (MQ=255)                                                                         aCCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacggcaacgg                                                                       <  2:55404/149‑1 (MQ=255)                                                                           cgcgCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTtcgtc                                                                                                  <  8:313082/120‑1 (MQ=255)                                                                           cgcgCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTtcgtc                                                                                                  >  7:313082/1‑120 (MQ=255)                                                                           cgcgCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacgg                                                                             >  1:328573/1‑141 (MQ=255)                                                                           cgcgCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacgg                                                                             <  2:328573/141‑1 (MQ=255)                                                                              gcCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGc                                                                  <  3:98120/149‑1 (MQ=255)                                                                                   ccTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGt                                                                                                                     <  2:314290/93‑1 (MQ=255)                                                                                   ccTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGt                                                                                                                     >  1:314290/1‑93 (MQ=255)                                                                                    cTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCat                                                            <  4:281203/149‑1 (MQ=255)                                                                                          cGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGa                                                                                                >  3:323393/1‑107 (MQ=255)                                                                                          cGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGa                                                                                                <  4:323393/107‑1 (MQ=255)                                                                                          cGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGc                                                      >  1:279376/1‑149 (MQ=255)                                                                                              gCCAACTGG‑TCCTTGTCGCCAAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGaaa                                                  <  1:1413/149‑1 (MQ=255)                                                                                                     gg‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCcgc                                           >  5:218628/1‑149 (MQ=255)                                                                                                      g‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCGGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCTAAAGTGCcgc                                           >  2:258265/1‑148 (MQ=255)                                                                                                      g‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCcgc                                           >  7:239606/1‑148 (MQ=255)                                                                                                       tgccttgccgccgcgggcgcgtctggtgctgctgggcgacaaggCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCcgc                                           >  7:300098‑M2/45‑148 (MQ=255)                                                                                                         ccttgccgccgcgggcgcgtctggtgctgctgggcgacaaggCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGtt                                                                    <  7:112402‑M2/79‑1 (MQ=255)                                                                                                         ccttgccgccgcgggcgcgtctggtgctgctgggcgacaaggCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGtt                                                                    >  8:112402‑M2/43‑121 (MQ=255)                                                                                                          CTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAg                                                                                         <  5:230735‑M1/99‑1 (MQ=255)                                                                                                          CTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAg                                                                                         >  6:230735‑M1/1‑99 (MQ=255)                                                                                                           TTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGACAGCATGATGGCGAAAGTGCCGCGAGcc                                      <  5:208457‑M1/149‑1 (MQ=255)                                                                                                             GTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGcc                                      >  6:218628‑M1/1‑147 (MQ=255)                                                                                                              tCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCgg                                   >  4:306011/1‑149 (MQ=255)                                                                                                              tCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCgg                                   >  1:163501/1‑149 (MQ=255)                                                                                                                  ccAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGggcgg                               >  5:134316/1‑149 (MQ=255)                                                                                                                           ccAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacg                                                                              <  4:60485/93‑1 (MQ=255)                                                                                                                           ccAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCcaacg                                                                              >  3:60485/1‑93 (MQ=255)                                                                                                                           ccAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGc                      <  5:220858/149‑1 (MQ=255)                                                                                                                             aGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAg                    >  7:26480/1‑149 (MQ=255)                                                                                                                             aGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAg                    <  3:306011/149‑1 (MQ=255)                                                                                                                                 gcgcCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAGGCGa                <  2:163501/149‑1 (MQ=255)                                                                                                                                       gcggcgGCAAGGCATCGAGCAGGTTGGCCATCATTTCCGGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCCACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAGGCGATGCACg          <  1:258265/149‑1 (MQ=255)                                                                                                                                               aaggCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAGGCGATGCACGGTGCTGAc  >  7:202760‑M2/5‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACCTGCTGAAGCCACGCTGCAGTCGCCGGCGAGTAAAAGCCCTCCTCAGCATCCCGGCACAGGTCACCCAGCACCGCGCCGGCCTCGACCGAGGCCAACTGG‑TCCTTGTCGCCCAGCAGCACCAGACGCGCCCGCGGCGGCAAGGCATCGAGCAGGTTGGCCATCATTTCCAGGTCGATCATCGACGCTTCGTCGACCACCAGCACATCCAACGGCAACGGGTTGCCAGCATGATGGCGAAAGTGCCGCGAGCCCGGGCGGCTGCCGAGCAGGCGATGCACGGTGCTGAC  >  NC_002947/5298948‑5299237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.