breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 137 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,327,1210CT64.0% 39.9 / 101.1 116intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_0029474,732,8850GA58.4% 40.4 / 80.1 78A917V (GCT→GTT) sucA2‑oxoglutarate dehydrogenase subunit E1
*NC_002947699,7220AG56.1% 12.1 / 95.1 82intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_0029475,948,1390GA55.1% 16.9 / 78.4 70A112V (GCC→GTC) rhotranscription termination factor Rho
*NC_0029472,772,3690CT51.9% ‑3.2 / 66.2 52R149R (CGG→CGAPP_2425AraC family transcriptional regulator
*NC_0029471,843,5350CT48.3% 1.0 / 75.4 60G420D (GGC→GAC) gacSsensor protein GacS
*NC_0029474,348,7050AG46.4% 36.4 / 102.9 84intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_0029474,621,0210A.45.1% 90.0 / 87.4 51coding (31/417 nt)PP_5654phosphotriesterase
*NC_002947195,6340CT38.9% 91.3 / 113.9 114T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_0029474,340,0890GC37.9% 13.9 / 23.5 30intergenic (+74/‑257)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
*NC_0029475,256,8640TA35.5% 28.1 / 18.8 31intergenic (+16/+29)PP_5703/PP_4634type III effector protein HopJ/alpha/beta family hydrolase
*NC_0029474,340,0830AG34.5% 26.1 / 19.2 30intergenic (+68/‑263)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
*NC_002947441,3260G.34.4% 62.4 / 37.1 33coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
*NC_0029474,340,0900AT33.3% 26.5 / 14.9 30intergenic (+75/‑256)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
*NC_0029475,256,8650TA32.3% 29.0 / 20.1 31intergenic (+17/+28)PP_5703/PP_4634type III effector protein HopJ/alpha/beta family hydrolase
*NC_0029475,256,8660TA32.3% 28.5 / 21.5 31intergenic (+18/+27)PP_5703/PP_4634type III effector protein HopJ/alpha/beta family hydrolase
*NC_0029474,340,0820CT32.1% 30.2 / 17.1 30intergenic (+67/‑264)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
*NC_0029471,506,9300TC31.6% 65.2 / 40.4 58intergenic (+51/‑66)petC/sspAubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A
*NC_0029471,544,4890CA31.1% 55.4 / 33.3 45intergenic (+48/+66)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
*NC_0029474,340,0840CT31.0% 33.2 / 17.2 30intergenic (+69/‑262)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein

Marginal new junction evidence (lowest skew 10 of 527 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 458602911 (0.160)30 (0.480) 21/258 3.1 53.5% intergenic (+112/+103) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 = 4586109 42 (0.670)intergenic (+192/+23) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 4808174 =4 (0.060)22 (0.430) 14/210 3.6 61.1% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988935 25 (0.490)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 4073915NA (NA)21 (0.320) 19/272 3.8 30.6% intergenic (‑453/+171) mdtA/PP_3586 multidrug transporter membrane fusion protein/transposase
?NC_002947 = 4302585 49 (0.720)intergenic (+1075/‑225) PP_3774/PP_5627 hypothetical protein/hypothetical protein
* ? NC_002947 1298266 =NA (NA)21 (0.320) 19/272 3.8 NA intergenic (+125/+304) PP_1133/PP_1134 transposase/hypothetical protein
?NC_002947 1298312 = NA (NA)intergenic (+171/+258) PP_1133/PP_1134 transposase/hypothetical protein
* ? NC_002947 = 5545666NA (NA)18 (0.270) 18/274 4.0 41.4% intergenic (‑806/+71) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NC_002947 5621244 = 26 (0.380)coding (20/774 nt) PP_4939 hypothetical protein
* ? NC_002947 4537544 =0 (0.000)20 (0.300) 16/270 4.4 88.9% coding (879/1533 nt) PP_4025 transposase
?NC_002947 = 4626651 5 (0.080)coding (853/1533 nt) PP_4091 transposase
* ? NC_002947 4537518 =NA (NA)20 (0.300) 16/270 4.4 100% coding (853/1533 nt) PP_4025 transposase
?NC_002947 4537544 = 0 (0.000)coding (879/1533 nt) PP_4025 transposase
* ? NC_002947 = 48081794 (0.060)18 (0.310) 13/240 4.6 49.7% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988949 33 (0.570)intergenic (+243/‑303) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 591418451 (0.750)11 (0.200) 10/224 5.2 24.2% intergenic (+76/+77) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
?NC_002947 = 5914224 28 (0.510)intergenic (+116/+37) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
* ? NC_002947 4647557 =28 (0.410)9 (0.180) 8/202 5.4 29.4% intergenic (+10/+121) fusB/PP_4112 elongation factor G 2/sulfate transporter
?NC_002947 4647665 = 23 (0.470)intergenic (+118/+13) fusB/PP_4112 elongation factor G 2/sulfate transporter