New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 111497	52 (1.230)	4 (0.100)	4/248	NT	7.5%	coding (1517/1590 nt)	ctaD	cytochrome c oxidase subunit 1
	?	NC_002947	= 111528	49 (1.210)					coding (1548/1590 nt)	ctaD	cytochrome c oxidase subunit 1

ACATGGTGTCGTCGATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTCGCGCTGGTGCCGATCTACGA  >  NC_002947/111380‑111674                                                                                                                                                     |                                                                                                                                                   aCATGGTGTCGTCGATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCt                                                                                                                                                    >  1:134371/1‑149 (MQ=255)    tGGTGTCGTCGATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCTGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTgcg                                                                                                                                                 >  8:141780‑M2/1‑146 (MQ=255)       tgtCGTCGATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGccc                                                                                                                                              <  2:18077/149‑1 (MQ=255)          cgtcgATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTAc                                                                                                                                           <  1:150161/149‑1 (MQ=255)             cgATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCATACcac                                                                                                                                        <  7:215152/149‑1 (MQ=255)                tCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACAcc                                                                                                                                     <  2:142263/149‑1 (MQ=255)                              ttCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACAccccc                                                                                                                       >  5:76877/1‑149 (MQ=255)                                        cGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGaaa                                                                                                             <  3:29840/149‑1 (MQ=255)                                         gCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGaaa                                                                                                             >  8:195356/1‑148 (MQ=255)                                         gCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAAt                                                                                                            <  3:215164/149‑1 (MQ=255)                                          gaGATCTTTTTCGTTTTCATTGTCATCAAGTGCATCCTCGGTGGTGCGTCGGTACGTGCTTAACCCTTGGANGTCGCCGTGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCGCTACCACACCTTCCAGACACCCCCGGAAGTGAAATa                                                                                                           <  1:86187/148‑1 (MQ=255)                                          cAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATa                                                                                                           <  7:195356/149‑1 (MQ=255)                                          cAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATa                                                                                                           <  2:198246/149‑1 (MQ=255)                                                ttcttcCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAAcc                                                                                                     >  2:146263/1‑149 (MQ=255)                                                     ccTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGcc                                                                                                 <  2:134371/148‑1 (MQ=255)                                                       tGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCAt                                                                                               >  2:137170/1‑148 (MQ=255)                                                                   tcaAGTGCATCCGCTGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCg                                                                                  <  2:166668/149‑1 (MQ=255)                                                                   tcaAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGccc                                                                                                                                              >  1:220168/1‑89 (MQ=255)                                                                   tcaAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGccc                                                                                                                                              <  2:220168/89‑1 (MQ=255)                                                                   tcaAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCg                                                                                  >  6:237351/1‑149 (MQ=255)                                                                   tcaAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCg                                                                                  >  6:241605/1‑149 (MQ=255)                                                                     aaGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCgg                                                                                 <  1:137170/148‑1 (MQ=255)                                                                     aaGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCgg                                                                                 <  2:73135/148‑1 (MQ=255)                                                                          cATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAAc                                                                           >  1:138385/1‑149 (MQ=255)                                                                            tCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGc                                                                         >  2:268310/1‑149 (MQ=255)                                                                              cgcgGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCt                                                                       <  1:268310/149‑1 (MQ=255)                                                                                cgGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTgg                                                                     <  5:241605/149‑1 (MQ=255)                                                                                cgGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTgg                                                                     <  5:237351/149‑1 (MQ=255)                                                                                      cgcgcCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAAcgc                                                               >  5:208105/1‑149 (MQ=255)                                                                                        cgcCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACgcgc                                                             >  7:91327/1‑149 (MQ=255)                                                                                        cgcCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACgcgc                                                             >  1:236848/1‑149 (MQ=255)                                                                                              cACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTg                                                       <  1:146263/149‑1 (MQ=255)                                                                                                 cGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACAccccc                                                                                                                       <  4:92137/82‑1 (MQ=255)                                                                                                     cAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGa                                                <  6:76877/149‑1 (MQ=255)                                                                                                         cccTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTc                                                                                   >  1:14260/1‑110 (MQ=255)                                                                                                         cccTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTc                                                                                   <  2:14260/110‑1 (MQ=255)                                                                                                          ccTGGGATGGCGCCGAGGGCCTGTAGTGGTCGATACCCTCGCCTgcgccatcccagggtttggccggtgccggcgcgccaccgcggatgcactggatgacaatgaacaggaagaagatctgcgaggcgccgaacatgaacgcgccgat                                            <  7:169045‑M2/148‑105 (MQ=255)                                                                                                               gATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGAt                                      <  2:236848/149‑1 (MQ=255)                                                                                                               gATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGAt                                      >  1:261605/1‑149 (MQ=255)                                                                                                                aTGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTgtgccatcccagggtttggccggtgccggcgcgccaccgcggatgcacttgatgacaatgaacaggaagaagatctgcgtggcgccgaacatgaacgcgccgatcgacga                                      <  2:210779‑M2/148‑111 (MQ=255)                                                                                                                    cgcCGAGGGCCTTGAGTGGTCGATACCCTCGCCTgcgccatcccagggtttggccggtgccggcgcgccaccgcggatgcacttgatgacaatgaacaggaagaagatctgcgtggcgccgaacatgaacgcgccgatcgacgacacca                                 <  7:162491‑M2/149‑116 (MQ=255)                                                                                                                    cgcCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCTGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCg                                 >  7:199153/1‑149 (MQ=255)                                                                                                                     gcCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGc                                >  1:13045/1‑149 (MQ=255)                                                                                                                      gcgtggggCTTGAGTGGTCGTTACCCTCGCCTGCGCCCTACCACACCTTCCAGAGACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGTTGATGTTCGcc                               <  2:261605/145‑1 (MQ=255)                                                                                                                        gAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCtt                             >  3:32956/1‑149 (MQ=255)                                                                                                                          gggCCTTGAGTGGTCGATACCCTCGCCTgtgccatcccagggtttggccggtgccggcgcgccaccgcggatgcacttgatgacaatgaacaggaagaagatctgcgtggcgccgaacatgaacgcgccgatcgacgacaccatgttga                           >  1:210779‑M2/1‑28 (MQ=255)                                                                                                                          gggCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCg                           <  6:208105/149‑1 (MQ=255)                                                                                                                           ggCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCgg                          <  4:32956/149‑1 (MQ=255)                                                                                                                              cTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCt                                                           >  2:166990/1‑113 (MQ=255)                                                                                                                              cTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCt                                                           <  1:166990/113‑1 (MQ=255)                                                                                                                              cTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCtt                       >  5:164153/1‑149 (MQ=255)                                                                                                                                 gAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTcgc                    >  2:5007/1‑149 (MQ=255)                                                                                                                                      gTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTCGCGCTgg               >  1:116133/1‑149 (MQ=255)                                                                                                                                       tCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGCAACGCCTGGTAACGCGCCTGTTGATGCTCACCGTGGAGATGTTCGCCTTCGGCTTCGCGCTGGt              >  5:265705/1‑149 (MQ=255)                                                                                                                                         gATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTCGCGCTGGTGc            >  6:141503/1‑149 (MQ=255)                                                                                                                                              ccTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCa                                                                                                <  1:154966/60‑1 (MQ=255)                                                                                                                                              ccTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCa                                                                                                >  2:154966/1‑60 (MQ=255)                                                                                                                                              ccTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGc                         >  8:103442/1‑131 (MQ=255)                                                                                                                                               atCGCCTGCGCCCTACCACACCTTCCAGACACCACCGGAAGTGAAATAGGAACCCGCCATGAACTGACTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGATGAAGGTGGTGATGTTCGCCTTCGGCTTCGCGCTGGTGCCGATc       >  4:82064/2‑148 (MQ=255)                                                                                                                                                   ccTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTCGCGCTGGTGCCGATCTacga  >  6:125487/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACATGGTGTCGTCGATCGGCGCGTTCATGTTCGGCGCCACGCAGATCTTCTTCCTGTTCATTGTCATCAAGTGCATCCGCGGTGGCGCGCCGGCACCGGCCAAACCCTGGGATGGCGCCGAGGGCCTTGAGTGGTCGATACCCTCGCCTGCGCCCTACCACACCTTCCAGACACCCCCGGAAGTGAAATAGGAACCCGCCATGAACGGCCTGTCGGTGAAACGCCTGGTAACGCGCCTGTTGATGCTGACGGTGGTGATGTTCGCCTTCGGCTTCGCGCTGGTGCCGATCTACGA  >  NC_002947/111380‑111674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.