Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 ← / ← PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5555608	2 (0.030)	45 (0.680) +GCC	38/278	1.1	95.8%	intergenic (‑84/+87)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer
	?	NC_002947	5555609 =	2 (0.030)					intergenic (‑85/+86)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

CGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555461‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATCATCCAGCACCTTG  >  NC_002947/5555609‑5555755                                                                                                                                                      |||                                                                                                                                                      CGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGG                                                                                                                                                         <  6:87396/149‑1      TCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCG                                                                                                                                                    >  7:42413/1‑149        ACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGG                                                                                                                                                   <  2:272506/148‑1         CTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCA                                                                                                                                                 <  4:49896/149‑1            TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATT                                                                                                                                              >  5:187870/1‑149                 GAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGC                                                                                                                                         <  6:93472/149‑1                  AAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGC                                                                                                                                         <  8:42413/148‑1                    ATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTG                                                                                                                                      >  7:20139/1‑149                        AGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTAT                                                                                                                                   <  4:48044/148‑1                              GCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACA                                                                                                                             >  7:221126/1‑148                                cgggtCAGCGATTTGTCGGGCGGACATGAGCACGTCCTTTTGTCCTTTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCATTGTGTCGCTAAAGCGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAAC                                                                                                                          <  6:18027/144‑1                                              GTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATG                                                                                                            >  2:96582/1‑149                                                  GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGGCATTTCGGCGTGTTTGGAACAAACCTGGGGCTGCTATGGAGC                                                                                                        >  1:280964/1‑149                                                   GCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCC                                                                                                       <  3:55601/149‑1                                                        CATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCATAGCAGCCCcagg                                                                                                  <  8:221126/149‑5                                                         ATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                                 <  1:96582/149‑1                                                         ATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                                 <  5:391159/149‑1                                                           GAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGGAAGGGCCCCCAAG‑CGGCCCCGGCAATTTCCGCGGGTATGCAAAAAAACTGGGGCTGGTATGAAGCCCTTTGGCG                                                                                               >  6:90112/1‑149                                                                 CTCCTTTTGTCATTTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAA                                                                                         <  1:335994/149‑1                                                                 CTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAA                                                                                         <  8:87670/149‑1                                                                  TCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCT                                                                                                               <  1:82348/127‑1                                                                  TCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCT                                                                                                               >  2:82348/1‑126                                                                        TGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCA                                                                                   <  7:339486/148‑1                                                                            ATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAG                                                                              <  6:187870/149‑1                                                                                          GCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTT                                                                <  2:30196/149‑1                                                                                            ATAGCAACACCGCTCAGGTGTGGGAGCGGCGTTTTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCA                                                              <  2:271387/149‑1                                                                                            ATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTT                                                                >  4:245489/1‑147                                                                                                     CCGCTCAGGTGTGGGAGGGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAA                                                     <  6:22952/149‑1                                                                                                     CCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAA                                                     <  5:90112/149‑1                                                                                                                  tGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTG                                       <  7:300509/148‑1                                                                                                                  GGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCGTTCGCGGCACCAGGCGCACAAAGGACCGCGCAGGCGTCAGGCCCGGAACT‑GGCCCCGGCTG                                       >  5:332487/1‑149                                                                                                                         CCTTGTGTCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCG                                 >  6:391911/1‑147                                                                                                                                TCGCGAAAGGGCCGCATAG‑CGGCCCCGGCGATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGC                        >  1:4495/1‑149                                                                                                                                TCGCGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGC                        >  5:125424/1‑149                                                                                                                                  GCGAAAGGGCCCCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCC                       <  7:88311/148‑1                                                                                                                                   CGAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGC                     >  3:73246/1‑149                                                                                                                                    GAAAGGGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGC                     >  5:391845/1‑148                                                                                                                                         GGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCA                   >  6:362315/1‑145                                                                                                                                         GGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCA                   <  5:362315/145‑1                                                                                                                                         GGCCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCGGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATC               <  7:196318/149‑1                                                                                                                                           CCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTCGGCCCAGGCTGGCGGCTCAGCTGCGCgcca                    <  1:36204/144‑5                                                                                                                                           CCGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGCGGCTCAGCTGCGCgcca                    >  2:36204/1‑139                                                                                                                                            CGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGGAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATCATC            >  3:224516/1‑149                                                                                                                                            CGCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATCATC            <  3:245489/149‑1                                                                                                                                             GCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAA                                                                               <  7:208646/83‑1                                                                                                                                             GCATAG‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAA                                                                               >  8:208646/1‑83                                                                                                                                                       CCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATCATCCAGCACCTTG  >  6:237324/1‑149                                                                                                                                                      |||                                                                                                                                                      CGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAG‑CG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555461‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACT‑GGCCCAGGCTGGC‑GCGCAGCTGAGCCGCCAGATCATCCAGCACCTTG  >  NC_002947/5555609‑5555755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.