New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	5270051 =	35 (0.650)	7 (0.140)	6/254	NT	15.3%	coding (288/1371 nt)	radA	DNA repair enzyme
	?	NC_002947	5270112 =	44 (0.850)					coding (227/1371 nt)	radA	DNA repair enzyme

GACATACAGGGCCGGCATGCCCACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGGTGTTCCAGGCCCCGC  >  NC_002947/5269964‑5270259                                                                                                                                                     |                                                                                                                                                      cagcggtcgcgctggctggacagggcagcaggcgcagatcaagaccctggccgaagtcagtgtcgaggaaatcccgcgtttcacgacCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTgg                                                                                                                                                       >  7:38483‑M1/88‑149 (MQ=255) cagcggtcgcgctggctggacagggcagcaggcgcagatcaagaccctggccgaagtcagtgtcgaggaaatcccgcgtttcacgacCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTgg                                                                                                                                                       >  5:239187‑M1/88‑149 (MQ=255)     ggtcgcgctggctggacagggcagcaggcgcagatcaagaccctggccgaagtcagtgtcgaggaaatcccgcgtttcacgacCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGt                                                                                                                                                   >  1:315221‑M1/84‑149 (MQ=255)              ggCATGCCCACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCgg                                                                                                                                          <  1:14352/149‑1 (MQ=255)              ggCATGCCCACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCAAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCgg                                                                                                                                          <  7:213174/149‑1 (MQ=255)                cATGCCCACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCAGATGCCGGGGTCACAGCCAATCAGCACAACCGAGCAATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGa                                                                                                                                        >  2:51954/1‑149 (MQ=255)                      cACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCt                                                                                                                                  >  6:329296/1‑149 (MQ=255)                       aCGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCgga                                                                                                                                         >  8:99392/1‑140 (MQ=255)                       aCGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCgga                                                                                                                                         <  7:99392/141‑2 (MQ=255)                                   cacaAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTc                                                                                                                     >  2:64694/1‑149 (MQ=255)                                      aaGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGc                                                                                                                  <  5:329296/149‑1 (MQ=255)                                              tAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGt                                                                                                            <  2:49597/147‑1 (MQ=255)                                                gCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGAtt                                                                                                                                      <  4:144936/119‑1 (MQ=255)                                                gCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGAtt                                                                                                                                      >  3:144936/1‑119 (MQ=255)                                                           gACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCATGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCct‑g                                                                                            >  6:206089/1‑149 (MQ=255)                                                           gACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCct‑g                                                                                            >  7:222410/1‑149 (MQ=255)                                                                 ccGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGcc                                                                                      <  1:64694/148‑1 (MQ=255)                                                                   gATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTg                                                                                   >  2:271931/1‑149 (MQ=255)                                                                   gATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGACCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTg                                                                                   >  6:169095/1‑149 (MQ=255)                                                                         ggggTCACCGCCAATCAGCACCACCGAGCAATACACCAGGCCGCAAACCAGCACGCGGTACAGTTCTTTGCTTCTGGTAGTGAAACGAGGGATTTGCTCGAAACTGACTTAGGCCAGGGTCTTGATCTGAGCCT‑TC‑TGCCCTTTccag                                                                              >  5:339066/1‑148 (MQ=255)                                                                             tCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCcagccagc                                                                         >  3:74323/1‑149 (MQ=255)                                                                              cACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAgcg                                                                        >  2:314536/1‑149 (MQ=255)                                                                                   acgaccagcagcaccgaactggaccgcgtgctgggtggcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGAcc                                                                   >  1:315221‑M2/67‑149 (MQ=255)                                                                                    cAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCGTCGACACTGACTTCGGCCAGGGGCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCg                                                                  >  5:262407/1‑149 (MQ=255)                                                                                     aaTCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCgc                                                                 >  1:67115/1‑149 (MQ=255)                                                                                       ccagcagcaccgaactggaccgcgtgctgggtggcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCgctg                                                               >  7:38483‑M2/63‑149 (MQ=255)                                                                                       ccagcagcaccgaactggaccgcgtgctgggtggcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCgctg                                                               >  5:239187‑M2/63‑149 (MQ=255)                                                                                        CAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCgctgc                                                              >  1:314536‑M1/1‑149 (MQ=255)                                                                                          gcagcaccgaactggaccgcgtgctgggtggcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGctgctg                                                            >  5:225965‑M2/60‑149 (MQ=255)                                                                                          gcagcaccgaactggaccgcgtgctgggtggcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGctgctg                                                            <  6:239187‑M2/90‑1 (MQ=255)                                                                                               accGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTgc                                                       <  7:48177/149‑1 (MQ=255)                                                                                                 cGAGCCAGCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTgcag                                                     <  1:58092/149‑1 (MQ=255)                                                                                                       aTCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAgcgc                                               >  4:178797/1‑149 (MQ=255)                                                                                                          caccaGGCCGCCACCCAGCACGCGGTCCTGTTCTGAGCTGCTGGTCGTGAAACGCGTGATTTCCTCGACACTGACTTCGGCCAGGGTCTTTATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCTCTGCTGCACCCAGCAgcggtc                                             <  6:262407/148‑4 (MQ=255)                                                                                                           accaGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGcgccgcc                                           >  2:79417/1‑149 (MQ=255)                                                                                                           accaGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGcgccgcc                                           >  2:247842/1‑149 (MQ=255)                                                                                                                 ccgccACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTc                                     <  1:247842/149‑1 (MQ=255)                                                                                                                 ccgccACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTc                                     <  3:178797/149‑1 (MQ=255)                                                                                                                   gccACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGa                                   <  5:206089/149‑1 (MQ=255)                                                                                                                   gccACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGa                                   <  7:33999/149‑1 (MQ=255)                                                                                                                      aCCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCa                                <  8:98411/149‑1 (MQ=255)                                                                                                                        gcggcctggtggatggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATg                              <  8:38483‑M2/120‑1 (MQ=255)                                                                                                                        ccAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTg                                                                                   >  5:76273/1‑96 (MQ=255)                                                                                                                        ccAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTg                                                                                   <  6:76273/96‑1 (MQ=255)                                                                                                                        ccAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATg                              >  1:213958/1‑149 (MQ=255)                                                                                                                        ccAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATg                              >  3:10197/1‑149 (MQ=255)                                                                                                                         cAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGcc                                                                                      <  5:30780/92‑1 (MQ=255)                                                                                                                         cAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGcc                                                                                      >  6:30780/1‑92 (MQ=255)                                                                                                                          aGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGt                            >  5:106608/1‑149 (MQ=255)                                                                                                                             aCGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTc                         >  1:191500/1‑149 (MQ=255)                                                                                                                               gcgGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGTATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATGTGCGCCTCTCTTGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTc                         <  1:271931/149‑1 (MQ=255)                                                                                                                               gcgGTCCAGTGCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGa                       <  8:222410/149‑1 (MQ=255)                                                                                                                                    atggctcggtggtgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTCCGATCATGGTTTCGACCAgg                  <  2:315221‑M2/132‑1 (MQ=255)                                                                                                                                      aGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGgt                <  2:67115/149‑1 (MQ=255)                                                                                                                                       gTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGgtg               >  8:279957/1‑149 (MQ=255)                                                                                                                                               ctgctgGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGGTGTTCCAGGc       <  2:213958/149‑1 (MQ=255)                                                                                                                                                     gTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGGTGTTCCAGGCCCCGc  <  4:74323/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GACATACAGGGCCGGCATGCCCACGGCAATGTTGCACAAGGTCTGTAGCAAAATGGTCGACTTGCCGATGCCGGGGTCACCGCCAATCAGCACCACCGAGCCATCCACCAGGCCGCCACCCAGCACGCGGTCCAGTTCGGTGCTGCTGGTCGTGAAACGCGGGATTTCCTCGACACTGACTTCGGCCAGGGTCTTGATCTGCGCCT‑GC‑TGCCCTGTCCAGCCAGCGCGACCGCTGCTGGGTGCAGCAGCGCCGCCGCTTTCGATCATGGTTTCGACCAGGGTGTTCCAGGCCCCGC  >  NC_002947/5269964‑5270259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.