New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1242577 =	36 (0.780)	5 (0.120)	5/260	2.9	12.9%	coding (504/915 nt)	ldcA	murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
	?	NC_000913	1242595 =	36 (0.880)					coding (486/915 nt)	ldcA	murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
Rejected: Frequency below cutoff threshold.

GTCTTCTGGCGCATGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTGGCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1242740‑1242577 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcccgacatgtcgggccTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCA  >  NC_000913/1242595‑1242727                                                                                                                                                                                                                                                                                                           GTCTTCTGGCGCATGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTGGCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCC                                                                                                                                                     >  1:483032/1‑150                    TGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTGGCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCCTTCCCC                                                                                                                                 <  2:233075/151‑1                            CCTTTAGCGGCCCCATGCTGGTAGCAAACTTTGGCGCGGATGAACTGCACGCCTTTACCGAGCACCATTTCTGTCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACACGTCGGGCCTTCCCCTTGCCATT                                                                                                                         >  1:601951/1‑151                                                                   ATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAAT                                                                                  <  2:376345/151‑1                                                                                               TTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCAT                                                       >  2:160943/1‑150                                                                                                    GGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCG                                                  >  2:679919/1‑150                                                                                                                                                    GCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCA  <  1:5489/150‑1                                                                                                                                                                                                                                                                                                           GTCTTCTGGCGCATGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTGGCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAAGGGGAAGGCCCGACATGTCGGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1242740‑1242577 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcccgacatgtcgggccTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCA  >  NC_000913/1242595‑1242727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.