| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | = 1821730 | 107 (0.950) | 5 (0.050) | 5/258 | 16.3 | 4.5% | coding (309/1119 nt) | ald2 | Alanine dehydrogenase 2 |
| ? | USA300TCH1516_ALE | = 1821772 | 108 (1.000) | coding (267/1119 nt) | ald2 | Alanine dehydrogenase 2 | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TAAGTTTTTGTAGGAACTCTGCGCCAACTTGAGCTGACATTCTTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > USA300TCH1516_ALE/1821585‑1821730‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcttgtATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGT < USA300TCH1516_ALE/1821772‑1821635 TAAGTTTTTGTAGGAACTCTGCGCCAACTTGAGCTGACATTCTTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAG < 4:145058/141‑1 GTTTTTGTAGGAACTCTGCGCCAACTTGAGCTGACATTCTTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTT > 4:117088/1‑141 ACTTGAGCTGACATTCTTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGAGCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGTATTATTCACTTATCTTCATTT > 3:228114/1‑141 TTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAA > 1:595131/1‑141 TGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAA < 4:228114/141‑1 TACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACAC < 2:595131/141‑1 GGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGA < 1:120602/141‑1 TTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAGAAATTAACACAAGCTTTGATAGATAGAACAGTAATTAGCATTGCATATGAGACTGGGCAGTTACCAGCCCGATCTTTACCATTGTTATCACCAATGAGCGAGG < 2:183608/141‑1 TTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGG < 2:914528/141‑1 TGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGT < 1:236399/141‑1 TGTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGT > 2:209118/1‑141 TGTATTATTCACTTAACTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCCCCAATGAGTGAGGT < 2:444322/141‑1 GTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTAT < 1:210353/77‑1 GTATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTAT > 2:210353/1‑77 TAAGTTTTTGTAGGAACTCTGCGCCAACTTGAGCTGACATTCTTCCTGCTACCTCACTCATTGGTGATAACAATGGTAAAGATCGGTCTGGTAACTGCACAGTCTCATATGCAATACTAATTACTTTTCTATCTATCAAAGCTTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > USA300TCH1516_ALE/1821585‑1821730‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcttgtATTATTCACTTATCTTCATTTAGCAAATGAAGAAAAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAGTTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGT < USA300TCH1516_ALE/1821772‑1821635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |