| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | 1702257 = | 112 (1.140) | 17 (0.200) | 9/138 | NT | 16.5% | intergenic (‑1/+48) | comEA/tylM1 | ComE operon protein 1/dTDP‑3‑amino‑3,6‑dideoxy‑alpha‑D‑glucopyranose N,N‑dimethyltransferase |
| ? | USA300TCH1516_ALE | 1702302 = | 75 (0.880) | intergenic (‑46/+3) | comEA/tylM1 | ComE operon protein 1/dTDP‑3‑amino‑3,6‑dideoxy‑alpha‑D‑glucopyranose N,N‑dimethyltransferase | |||||
Only 100 of 151 total aligned reads displayed.
AATTAATACCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTCATTA > USA300TCH1516_ALE/1702167‑1702347 | tttaaacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagt < 1:2465183‑M1/1‑1 (MQ=255)tttaaacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagt > 1:2901145‑M1/91‑91 (MQ=255) aaacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTaaa > 1:2658766‑M1/88‑91 (MQ=255) aacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTaaaa > 1:4107756‑M1/87‑91 (MQ=255) aacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTaaaa > 1:534043‑M1/87‑91 (MQ=255) aacatgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTaaaa < 1:2889195‑M1/5‑1 (MQ=255) aaTACCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATaaaa < 1:2168034/91‑1 (MQ=255) tACCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAAcc < 1:367328/91‑1 (MQ=255) tACCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAAcc < 1:1467367/91‑1 (MQ=255) aCCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCa < 1:3651569/91‑1 (MQ=255) tgttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCAc < 1:3641710‑M1/9‑1 (MQ=255) ccATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCAc > 1:106284/1‑91 (MQ=255) ccATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCAc > 1:3902426/1‑91 (MQ=255) ccATAATTAAAACAACAGCACTTAGAATATAGAACTTCCATTGAGTTAAAAAATCTTAATAGCGTAATAAAAATTGATACAATAAAACCAc > 1:3685757/1‑91 (MQ=255) gttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCAct < 1:3522143‑M1/10‑1 (MQ=255) gttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCAct > 1:3744828‑M1/82‑91 (MQ=255) gttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCAct < 1:2358355‑M1/10‑1 (MQ=255) ttgaaacatttactgattttaatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCActc < 1:2727495‑M1/11‑1 (MQ=255) aTTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCActcctc < 1:1981214/91‑1 (MQ=255) aTTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCActcctc < 1:2862189/91‑1 (MQ=255) tAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCGCaa > 1:3734735/1‑91 (MQ=255) aaaaCAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAAc < 1:2543606/91‑1 (MQ=255) aacaacAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACtt > 1:1920783/1‑91 (MQ=255) aacaacAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACtt > 1:2766628/1‑91 (MQ=255) aacaacAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACtt > 1:3954976/1‑91 (MQ=255) aacaacAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACtt < 1:1336794/91‑1 (MQ=255) caacaGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTta < 1:2446935/91‑1 (MQ=255) caacaGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTta < 1:2758068/91‑1 (MQ=255) aacaCCACTTAGAATATATAACTTCCATTGAGTT‑AAAAATCTTTATCGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTtata > 1:3274069/1‑91 (MQ=255) acaGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTtata > 1:334959/1‑91 (MQ=255) acaGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTtata > 1:903259/1‑91 (MQ=255) caGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATac < 1:1143477/91‑1 (MQ=255) aGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATaca < 1:1016789/91‑1 (MQ=255) cACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACg > 1:1583347/1‑91 (MQ=255) cACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACg > 1:1943563/1‑91 (MQ=255) aCTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGt > 1:1078911/1‑91 (MQ=255) cTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTc < 1:1386325/91‑1 (MQ=255) tAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTg < 1:2651045/91‑1 (MQ=255) aatatagatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTg > 1:1857118‑M1/61‑91 (MQ=255) gatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg > 1:3932170‑M1/55‑91 (MQ=255) gatgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg > 1:472564‑M1/55‑91 (MQ=255) atataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg < 1:926644/91‑1 (MQ=255) atataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg < 1:3840006/91‑1 (MQ=255) atataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg < 1:3840103/91‑1 (MQ=255) atataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAg < 1:1876038/91‑1 (MQ=255) tataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGt > 1:864361/1‑91 (MQ=255) atgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGt > 1:834576‑M1/54‑91 (MQ=255) tgaacataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTg > 1:3388693‑M1/53‑91 (MQ=255) ataACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTg > 1:3498471/1‑91 (MQ=255) taACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTgg > 1:737653/1‑91 (MQ=255) aaCTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGt > 1:967566/1‑91 (MQ=255) aaCTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGACTGGt > 1:3453518/1‑91 (MQ=255) aCTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGtt < 1:3607415/91‑1 (MQ=255) aCTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGtt > 1:2222500/1‑91 (MQ=255) cataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGttt > 1:438586‑M1/49‑91 (MQ=255) cataatgaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGttt > 1:731560‑M1/49‑91 (MQ=255) aTTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACtt < 1:1237244/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCTACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:3591009/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt > 1:2306283/1‑91 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:2578598/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:919971/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:2187860/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:2186208/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt < 1:1959228/91‑1 (MQ=255) ttGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTCATACACGTCTGAGCGAGTGGTTTTACttt > 1:2306033/1‑91 (MQ=255) gaagatgcagaaagattgtttttcattgcgaaaaaataaaagTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACttt > 1:1644517‑M1/43‑91 (MQ=255) tGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACtttt > 1:1330573/1‑91 (MQ=255) gTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAtt > 1:311874/1‑91 (MQ=255) gTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAtt < 1:84249/91‑1 (MQ=255) tAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAtttt < 1:940526/91‑1 (MQ=255) tAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAtttt < 1:940543/91‑1 (MQ=255) aaaaaaTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAttttt > 1:1788678/1‑91 (MQ=255) aaTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGc < 1:2803283/91‑1 (MQ=255) aaTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGc < 1:3386550/91‑1 (MQ=255) aaTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGc > 1:3243438/1‑91 (MQ=255) aaTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGc < 1:2004467/91‑1 (MQ=255) aTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCa > 1:1417299/1‑91 (MQ=255) aTCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCa > 1:4039224/1‑91 (MQ=255) aTCTTTACAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTAGTTTTTCGCa < 1:2082624/91‑1 (MQ=255) cTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAAt > 1:3411449/1‑91 (MQ=255) cTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAAt > 1:215387/1‑91 (MQ=255) ttATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGa > 1:1019583/1‑91 (MQ=255) ttATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGa < 1:498103/91‑1 (MQ=255) tataGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGaa > 1:2222269/1‑91 (MQ=255) gCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTTGCAATGAAAAAc < 1:3185625/91‑1 (MQ=255) gTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACaa > 1:2312860/1‑91 (MQ=255) gTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACaa < 1:2731412/91‑1 (MQ=255) gTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACaa < 1:1403141/91‑1 (MQ=255) taataaAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAAt < 1:2612321/91‑1 (MQ=255) aataaAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATc < 1:3978845/91‑1 (MQ=255) ataaAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCt > 1:138189/1‑91 (MQ=255) aaaaTTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTc > 1:4031178/1‑91 (MQ=255) aaTTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTg < 1:483873/91‑1 (MQ=255) aTTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGc < 1:1437650/91‑1 (MQ=255) ttGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCa > 1:318641/1‑91 (MQ=255) gATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATc > 1:2433919/1‑91 (MQ=255) aCAATAAAATCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTc > 1:2088238/1‑91 (MQ=255) aCAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTc < 1:1634739/91‑1 (MQ=255) aaTAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTCAt < 1:3211559/91‑1 (MQ=255) TAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTCATTa > 1:1569281‑M1/1‑91 (MQ=255) | AATTAATACCATAATTAAAACAACAGCACTTAGAATATATAACTTCCATTGAGTTAAAAAATCTTTATAGCGTAATAAAAATTGATACAATAAAACCACTCCTCAACTTTATACACGTCTGAGCGAGTGGTTTTACTTTTATTTTTTCGCAATGAAAAACAATCTTTCTGCATCTTCATTA > USA300TCH1516_ALE/1702167‑1702347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |