| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | 2492466 = | 142 (0.700) | 25 (0.140) | 13/138 | NT | 18.6% | intergenic (+9/+37) | yhfP/rimI_4 | Putative quinone oxidoreductase YhfP/Ribosomal‑protein‑alanine acetyltransferase |
| ? | USA300TCH1516_ALE | 2492499 = | 96 (0.550) | intergenic (+42/+4) | yhfP/rimI_4 | Putative quinone oxidoreductase YhfP/Ribosomal‑protein‑alanine acetyltransferase | |||||
Only 100 of 252 total aligned reads displayed.
TGAACTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGGTATAAATCCAAA > USA300TCH1516_ALE/2492376‑2492556 | cagactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaa < 2:3266276‑M1/1‑1 (MQ=255) agactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaa < 2:3950276‑M1/2‑1 (MQ=255) agactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaa < 1:3437399‑M1/2‑1 (MQ=255) gactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaa < 1:1020134‑M1/3‑1 (MQ=255) gactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaa > 1:1238490‑M1/89‑91 (MQ=255) aactacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaa > 2:2373558‑M1/89‑91 (MQ=255) actacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaaa < 2:2410546‑M1/4‑1 (MQ=255) aCTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGaaaa > 1:1124639/1‑91 (MQ=255) aCTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGaaaa > 2:155560/1‑91 (MQ=255) ctacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaaaa < 1:3168421‑M1/5‑1 (MQ=255) ctacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaaaa < 2:226656‑M1/5‑1 (MQ=255) ctacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaaaa > 1:2645371‑M1/87‑91 (MQ=255) ctacccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaaaaaa < 1:3210222‑M1/5‑1 (MQ=255) cTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGtaaag > 1:504472/1‑90 (MQ=255) cTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGCCGCATTGTTATCGATTTCGGTGTAGATAAATAGAAATCATGaaaaa > 2:1258978/1‑91 (MQ=255) ttCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAg < 1:2331179/91‑1 (MQ=255) tCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGa < 1:1838390/91‑1 (MQ=255) tCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGa < 2:1737067/91‑1 (MQ=255) acccagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGa < 1:1406617‑M1/7‑1 (MQ=255) ccAGAACAACTTAACAAAGTAATTAAACATGAAAATAAATGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGAc > 1:2410546/1‑91 (MQ=255) ccAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGAc > 1:2159332/1‑91 (MQ=255) cAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACa > 2:1179940/1‑91 (MQ=255) cagcggataaattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACAt < 1:2055819‑M1/10‑1 (MQ=255) gAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATc > 2:3022932/1‑91 (MQ=255) aacaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATcc < 2:1626203/91‑1 (MQ=255) aacaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATcc > 2:3713715/1‑91 (MQ=255) aacaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATcc < 1:2318390/91‑1 (MQ=255) acaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATccc < 2:1814330/91‑1 (MQ=255) acaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATccc < 1:689430/91‑1 (MQ=255) acaacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATccc > 1:2574298/1‑91 (MQ=255) aacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGt > 2:3590850/1‑91 (MQ=255) aacTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGt > 2:3590824/1‑91 (MQ=255) acTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGtt < 2:1867760/91‑1 (MQ=255) acTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGtt > 1:3744228/1‑91 (MQ=255) aattatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATg > 2:538670‑M1/73‑91 (MQ=255) attatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaaataAAAAAGACATCCCGTTATGc > 2:1684012‑M1/72‑91 (MQ=255) ttatatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCg > 1:1906555‑M1/71‑91 (MQ=255) aCAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCg < 1:3332743/91‑1 (MQ=255) atatacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCgag > 1:1614122‑M1/69‑91 (MQ=255) atatacaaaagttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCgag > 2:2831391‑M1/69‑91 (MQ=255) aaaGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCgag < 2:209819/91‑1 (MQ=255) aaGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGATATGCgaga > 2:1051420/1‑91 (MQ=255) atacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGAt > 1:72081‑M1/67‑91 (MQ=255) tacaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGATg > 2:2488780‑M1/66‑91 (MQ=255) gaaTTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGt < 2:3869910/90‑1 (MQ=255) acaaaatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGATGt > 1:566638‑M1/65‑91 (MQ=255) aTTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCt < 2:2569025/91‑1 (MQ=255) aatttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGATGTCttt > 1:1047472‑M1/61‑91 (MQ=255) atttggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGATGTCtttt > 1:2702315‑M1/60‑91 (MQ=255) aaaCATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCtttt > 2:3805342/1‑91 (MQ=255) aaCATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCTCGTTATGCGAGATGTCttttt > 2:934610/1‑91 (MQ=255) aCATGGAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCtttttt < 2:3201706/91‑1 (MQ=255) cATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTa > 2:2978974/1‑91 (MQ=255) ggatttatacctaccgaaccagattcaggcggtatgtatatcaaatactaaattaAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTaa > 1:1058789‑M1/56‑91 (MQ=255) tGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAAt < 1:80669/91‑1 (MQ=255) gAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAAtt > 2:1927831/1‑91 (MQ=255) aaaTAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTa < 1:2694143/91‑1 (MQ=255) aaaTAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTa < 1:835721/91‑1 (MQ=255) aTAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGt > 1:1916769/1‑91 (MQ=255) tAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTa < 1:3013660/91‑1 (MQ=255) aaaGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTAt < 1:391622/91‑1 (MQ=255) aaaGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTAt < 1:516638/91‑1 (MQ=255) aaGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTAtt > 1:1508340/1‑91 (MQ=255) aGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTAttt > 2:1156000/1‑91 (MQ=255) gggCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTg > 1:2499937/1‑91 (MQ=255) gggCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTg > 2:3165279/1‑91 (MQ=255) gcgcATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGCTGTCTTTTTTAATTTAGTATTTGAT > 1:3685045‑M1/1‑91 (MQ=255) gcgcATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGAT < 1:3735244‑M1/91‑1 (MQ=255) gcgcATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGAT > 2:2311768‑M1/1‑91 (MQ=255) cgcATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATA > 2:3840304‑M1/1‑91 (MQ=255) gcATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATAT < 1:1537203‑M1/91‑1 (MQ=255) cATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATA < 2:3375009‑M1/91‑1 (MQ=255) cATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATA < 2:221577‑M1/91‑1 (MQ=255) aTTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATAC < 1:608014‑M1/91‑1 (MQ=255) aTTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTAGATATAC > 2:3518596‑M1/1‑91 (MQ=255) gTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATatacata > 1:2823625/1‑91 (MQ=255) cGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGcc < 2:2937286/91‑1 (MQ=255) cGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGcc > 1:350827/1‑91 (MQ=255) cGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGcc < 2:678715/91‑1 (MQ=255) tttCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGa < 2:2226959/91‑1 (MQ=255) tCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAAt > 1:1729381/1‑91 (MQ=255) cGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGaagc < 2:670782/91‑3 (MQ=255) cGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATc < 2:2601875/91‑1 (MQ=255) cGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATc < 1:3549791/91‑1 (MQ=255) gtgtAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTg < 1:2527171/91‑1 (MQ=255) gtgtAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTg > 1:2714215/1‑91 (MQ=255) tgtAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTgg > 2:1407050/1‑91 (MQ=255) tgtAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTgg > 1:1384651/1‑91 (MQ=255) tAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGtt > 1:2366300/1‑91 (MQ=255) aGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTc > 2:572464/1‑91 (MQ=255) aaaTAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCggta > 2:418669/1‑91 (MQ=255) aGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGgta < 2:1961474‑M2/91‑4 (MQ=255) gTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGgtat < 2:3342125‑M2/91‑5 (MQ=255) tATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACctaccgaaccagattcaggcggtatgtata < 2:588265‑M1/91‑31 (MQ=255) tATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGgtata < 1:951475‑M2/91‑6 (MQ=255) tCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGGTATAAAt < 2:3719745/91‑1 (MQ=255) tCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGGTATAAAt < 1:1284831/91‑1 (MQ=255) cATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACctaccgaaccagattcaggcggtatgtatatcaa < 1:1630206‑M1/91‑35 (MQ=255) cATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGGTATAAATc < 2:2015319/91‑1 (MQ=255) gtGAAAGGGACGTCGCGTCACGCGAGATGTCTTTTGTAATTTAGTATGTGAGAGACATACCGCCTGAAGCTGGTTCGGTAGGTATAAAGcc < 2:1324354/90‑1 (MQ=255) | TGAACTTCCAGAACAACTTAACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAGTATTCATGAAAAAGACATCCCGTTATGCGAGATGTCTTTTTTAATTTAGTATTTGATATACATACCGCCTGAATCTGGTTCGGTAGGTATAAATCCAAA > USA300TCH1516_ALE/2492376‑2492556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |