breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
MC | CP000731 | 1 | Δ27,041 bp | repA–USA300HOU_pUSA300HOUMR0033 | 26 genes repA, USA300HOU_pUSA300HOUMR0003, USA300HOU_pUSA300HOUMR0004, USA300HOU_pUSA300HOUMR0005, USA300HOU_pUSA300HOUMR0006, cadR, cadX, USA300HOU_pUSA300HOUMR0010, blaZ, blaR, blaI, USA300HOU_pUSA300HOUMR0014, USA300HOU_pUSA300HOUMR0015, USA300HOU_pUSA300HOUMR0016, USA300HOU_pUSA300HOUMR0018, USA300HOU_pUSA300HOUMR0019, USA300HOU_pUSA300HOUMR0021, USA300HOU_pUSA300HOUMR0023, USA300HOU_pUSA300HOUMR0024, USA300HOU_pUSA300HOUMR0027, USA300HOU_pUSA300HOUMR0028, USA300HOU_pUSA300HOUMR0029, USA300HOU_pUSA300HOUMR0030, USA300HOU_pUSA300HOUMR0031, USA300HOU_pUSA300HOUMR0032, USA300HOU_pUSA300HOUMR0033 |
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MC | NC_012417 | 1 | Δ3,125 bp | USA300HOU_RS14890–USA300HOU_RS14900 | USA300HOU_RS14890, USA300HOU_RS15665, USA300HOU_RS14895, USA300HOU_RS14900 | |
RA | USA300TCH1516_ALE | 39,378 | C→T | D586N (GAT→AAT) | pbp ← | Beta‑lactam‑inducible penicillin‑binding protein |
RA | USA300TCH1516_ALE | 367,644 | G→T | intergenic (‑96/‑62) | nanA ← / → bglK | N‑acetylneuraminate lyase/Beta‑glucoside kinase |
RA | USA300TCH1516_ALE | 406,961 | C→G | A353G (GCA→GGA) | USA300HOU_RS01880 → | putative acetyl‑CoA acyltransferase |
RA | USA300TCH1516_ALE | 479,553 | G→A | A194T (GCA→ACA) | USA300HOU_RS02280 → | hypothetical protein |
RA | USA300TCH1516_ALE | 638,614 | G→T | T14K (ACA→AAA) | pdxK ← | Pyridoxine kinase |
RA | USA300TCH1516_ALE | 646,838 | G→A | intergenic (+192/‑395) | lipL → / → galK | Octanoyl‑[GcvH]:protein N‑octanoyltransferase/Galactokinase |
RA | USA300TCH1516_ALE | 654,252 | C→T | S360L (TCG→TTG) | USA300HOU_RS03120 → | Ribonuclease |
RA | USA300TCH1516_ALE | 707,879 | A→T | L11I (TTA→ATA) | znuC_1 ← | High‑affinity zinc uptake system ATP‑binding protein ZnuC |
RA | USA300TCH1516_ALE | 790,778 | G→A | intergenic (+277/‑197) | kipA_1 → / → ltaS | KipI antagonist/Lipoteichoic acid synthase |
RA | USA300TCH1516_ALE | 942,060 | C→G | intergenic (‑31/+218) | ptlE ← / ← mnhG1 | Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1 |
RA | USA300TCH1516_ALE | 942,163 | (A)6→5 | intergenic (‑134/+115) | ptlE ← / ← mnhG1 | Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1 |
RA | USA300TCH1516_ALE | 1,087,670 | A→T | L5I (TTA→ATA) | ykoE ← | Putative HMP/thiamine permease protein YkoE |
RA | USA300TCH1516_ALE | 1,232,465 | G→C | A124P (GCG→CCG) | prkC → | Serine/threonine‑protein kinase PrkC |
RA | USA300TCH1516_ALE | 1,416,458 | A→T | I216N (ATT→AAT) | oppD_4 ← | Oligopeptide transport ATP‑binding protein OppD |
RA | USA300TCH1516_ALE | 1,420,434 | G→A | A229T (GCG→ACG) | pepF1_2 → | Oligoendopeptidase F, plasmid |
RA | USA300TCH1516_ALE | 1,440,007 | Δ1 bp | coding (34/201 nt) | cspA_2 ← | Cold shock protein CspA |
RA | USA300TCH1516_ALE | 1,474,639 | C→G | A9129P (GCA→CCA) | ebh_1 ← | Extracellular matrix‑binding protein ebh |
RA | USA300TCH1516_ALE | 1,481,355 | G→T | A6890E (GCA→GAA) | ebh_1 ← | Extracellular matrix‑binding protein ebh |
RA | USA300TCH1516_ALE | 1,513,930 | A→T | L413F (TTA→TTT) | USA300HOU_RS07375 → | hypothetical protein |
RA | USA300TCH1516_ALE | 1,530,723 | G→A | T184I (ACA→ATA) | bshA ← | N‑acetyl‑alpha‑D‑glucosaminyl L‑malate synthase |
RA | USA300TCH1516_ALE | 1,646,189 | G→C | A155G (GCA→GGA) | ypdF ← | Aminopeptidase YpdF |
RA | USA300TCH1516_ALE | 1,744,454 | C→T | G73D (GGC→GAC) | apt ← | Adenine phosphoribosyltransferase |
RA | USA300TCH1516_ALE | 1,775,556 | G→A | P323L (CCT→CTT) | clpX ← | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
RA | USA300TCH1516_ALE | 1,797,772 | G→C | R7G (CGC→GGC) | phoR ← | Alkaline phosphatase synthesis sensor protein PhoR |
RA | USA300TCH1516_ALE | 1,834,119 | G→T | intergenic (+68/‑46) | osmC → / → USA300HOU_RS09140 | Peroxiredoxin OsmC/Soluble hydrogenase 42 kDa subunit |
RA | USA300TCH1516_ALE | 2,010,363 | C→T | C146Y (TGT→TAT) | USA300HOU_RS10125 ← | Putative multidrug export ATP‑binding/permease protein |
RA | USA300TCH1516_ALE | 2,027,561 | G→C | T129S (ACC→AGC) | vraS ← | Sensor protein VraS |
RA | USA300TCH1516_ALE | 2,035,886 | T→C | intergenic (+386/‑3) | ftnA → / → polC_2 | Bacterial non‑heme ferritin/DNA polymerase III PolC‑type |
RA | USA300TCH1516_ALE | 2,279,705 | G→A | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
RA | USA300TCH1516_ALE | 2,532,298 | A→T | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
RA | USA300TCH1516_ALE | 2,664,466 | G→A | A65T (GCG→ACG) | pitA_2 → | L‑methionine sulfoximine/L‑methionine sulfone acetyltransferase |
RA | USA300TCH1516_ALE | 2,679,708 | G→A | W64* (TGG→TGA) | ssaA2_4 → | Staphylococcal secretory antigen ssaA2 |
RA | USA300TCH1516_ALE | 2,719,269 | (A)5→6 | coding (213/705 nt) | cpnA → | Cyclopentanol dehydrogenase |
RA | USA300TCH1516_ALE | 2,856,087 | T→A | P80P (CCT→CCA) | rpoA_2 → | DNA‑directed RNA polymerase subunit alpha |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | USA300TCH1516_ALE | 512620 = | 48 (1.270) | 27 (0.990) +40 bp |
19/214 | 0.2 | 43.6% | intergenic (+688/‑6075) | recR/speA_2 | Recombination protein RecR/Arginine decarboxylase |
? | USA300TCH1516_ALE | 1998278 = | NA (NA) | intergenic (‑6/+3329) | USA300TCH1516_01882/USA300TCH1516_01883 | tRNA‑Val/tRNA‑Ala |