| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | = 492723 | 99 (0.520) | 29 (0.190) | 16/128 | NT | 24.9% | intergenic (+145/+40) | sle1_1/USA300HOU_RS02345 | N‑acetylmuramoyl‑L‑alanine amidase sle1/hypothetical protein |
| ? | USA300TCH1516_ALE | = 492741 | 96 (0.630) | intergenic (+163/+22) | sle1_1/USA300HOU_RS02345 | N‑acetylmuramoyl‑L‑alanine amidase sle1/hypothetical protein | |||||
Only 100 of 289 total aligned reads displayed.
AGTTGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTT > USA300TCH1516_ALE/492633‑492813 | agttgttactttaataacaaacggtgctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGaa < 1:1898198‑M1/16‑1 (MQ=255)aGTTGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAA > 1:2898562‑M1/1‑91 (MQ=255) gTTGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATAAGAAAAAGGATTGGTCGAAt < 2:164067/91‑1 (MQ=255) gTTGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGAATATGAAAAAGGATTGGTCGAAt < 2:1571317/91‑1 (MQ=255) ttGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATg < 1:1638432/91‑1 (MQ=255) ggATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGaa < 1:3044236/91‑1 (MQ=255) aTTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTACTAGGATTTTTTGTATATGAAAAAGGATTGGTCGAATGTACt > 1:1359840/1‑91 (MQ=255) tGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTc > 1:3126809/1‑91 (MQ=255) aaTCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCAt > 1:843963/1‑91 (MQ=255) aaTCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCAt < 2:3067609/91‑1 (MQ=255) cTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTc < 1:650631/91‑1 (MQ=255) aaTTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAAcatagaacatgaagttc < 1:3171156‑M1/91‑18 (MQ=255) aaTTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTAt < 1:2820933/91‑1 (MQ=255) ttttATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGt < 1:538426‑M2/91‑2 (MQ=255) tttATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAAcatagaacatgaagttcatt < 2:616309‑M1/91‑21 (MQ=255) acggtgctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGtt > 2:2301010‑M1/56‑91 (MQ=255) ttATGGCGGGGCTATCAGAGTCGTGATTGGTGTAATAGGAATTTTTGTATATGGAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttc < 2:895475‑M2/91‑4 (MQ=255) cggtgctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTc > 1:410932‑M1/55‑91 (MQ=255) cggtgctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTc > 2:2827516‑M1/55‑91 (MQ=255) gtgctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGa > 2:1316920‑M1/53‑91 (MQ=255) aTGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcga < 2:1900997‑M2/91‑6 (MQ=255) tGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgac < 2:185649‑M2/91‑7 (MQ=255) tGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgac > 2:2311837‑M2/1‑85 (MQ=255) ggCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgacc > 1:3756897‑M2/1‑84 (MQ=255) gctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGAcc > 2:2103822‑M1/51‑91 (MQ=255) gctagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGAcc > 1:2892261‑M1/51‑91 (MQ=255) ctagattatttacaagcattcgcaaggtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCa > 1:3620902‑M1/50‑91 (MQ=255) tagattatttacaagcattcgcaaagtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCaa > 2:1370861‑M1/49‑91 (MQ=255) ggggCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaat > 1:3578389‑M2/1‑81 (MQ=255) gCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatcct > 1:2530551‑M2/1‑78 (MQ=255) gCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatcct > 2:2733844‑M2/1‑78 (MQ=255) tATCAAAATCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatccttt > 2:3438493‑M2/1‑76 (MQ=255) tCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTc < 1:2825911/91‑1 (MQ=255) tCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTc > 2:1161813/1‑91 (MQ=255) cAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCt > 2:946631/1‑91 (MQ=255) aaaGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTg < 1:1736770/91‑1 (MQ=255) aGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGaa > 1:2602972/1‑91 (MQ=255) aGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGaa > 1:2454168/1‑91 (MQ=255) tGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGt < 2:3304313/91‑1 (MQ=255) aTTGTTGTAATAGAAATTTTTGTATATGAAAAAGGTTTGGTCGAATGAACTTCAGGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTAt < 2:1083309/91‑1 (MQ=255) ttgttgTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTAtt < 1:532870/91‑1 (MQ=255) agtaaaacatgaatactaattcagAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATg > 2:1291115‑M1/25‑91 (MQ=255) gtAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatccttttcatatacaaaaattccta < 1:2142546‑M2/91‑35 (MQ=255) tAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTa < 1:2654687/91‑1 (MQ=255) aGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTAc < 2:2787642/91‑1 (MQ=255) gAATTTTTGTATAAGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatcctttttcttatacaaaaattcctattacaa > 2:3770889‑M2/1‑50 (MQ=255) aaTTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaac < 2:2070350‑M2/91‑43 (MQ=255) aaTTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACttt < 2:2638870/91‑1 (MQ=255) aTTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTg > 1:1154082/1‑91 (MQ=255) aTTTTTGAACATGAAAAAGGATTGGTCGAATGTACTTCATGTTCTATGTTCGACCAATCCTGTTCTGAATAAGTATGCATGTTTTACTTTg < 2:3457354/91‑1 (MQ=255) ttttGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCg < 2:547761/91‑1 (MQ=255) ttttGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCg < 2:3691524/91‑1 (MQ=255) ttttGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCg < 2:690333/91‑1 (MQ=255) ttGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGaa < 2:2186003/91‑1 (MQ=255) tGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAAt > 1:1754909/1‑91 (MQ=255) tGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAAt < 1:1036553/91‑1 (MQ=255) tGTATATGAAAAAGGATTGGACGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGCATTAGTATTCATGTTTTACTTTGCGAAt > 1:2496185/1‑91 (MQ=255) gTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATg < 1:1431500/91‑1 (MQ=255) gTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATg > 2:415714/1‑91 (MQ=255) tataTGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGc < 1:45867/91‑1 (MQ=255) atatGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCt > 1:1508589/1‑91 (MQ=255) atatGAAAAAGGATTGGTCGAATGAACTTCATGCTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCt < 1:1252635/91‑1 (MQ=255) tatGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCtt > 2:3012155/1‑91 (MQ=255) atGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTg < 2:1476532/91‑1 (MQ=255) tGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGt < 2:2902006/91‑1 (MQ=255) aaaaaGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTaa > 2:631097/1‑91 (MQ=255) AAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAaat > 2:3234883‑M1/1‑91 (MQ=255) aGGATTGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagc > 2:154915‑M1/1‑13 (MQ=255) aGGATTGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagc > 1:2530251‑M1/1‑13 (MQ=255) aGGATTGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagc > 2:2733803‑M1/1‑13 (MQ=255) AGGATTGGTGGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAaataa > 1:3718458‑M1/1‑91 (MQ=255) GATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGaa < 2:1757241‑M1/76‑1 (MQ=255) agTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCt > 2:2926943‑M1/3‑91 (MQ=255) aTTGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagcccc > 1:3576265‑M1/1‑10 (MQ=255) ttGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccg < 1:1134560‑M1/91‑83 (MQ=255) ttGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccg > 1:2443167‑M1/1‑9 (MQ=255) ttGGTCGAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccg < 1:3169963‑M2/91‑65 (MQ=255) TTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCTa > 1:2770446‑M1/1‑91 (MQ=255) tGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgc > 1:214122‑M1/1‑8 (MQ=255) tGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgc > 1:2075602‑M1/1‑8 (MQ=255) tGGTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgc > 1:3361395‑M1/1‑8 (MQ=255) ggTCGAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgcc < 2:2087150‑M2/91‑67 (MQ=255) ggTCGAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgcc < 1:2655957‑M2/91‑67 (MQ=255) GGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCTAGc > 2:530586‑M1/1‑91 (MQ=255) gTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgcca > 2:2311770‑M1/1‑6 (MQ=255) gTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgcca < 2:670005‑M1/91‑86 (MQ=255) gTCGAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgcca < 2:185633‑M1/91‑86 (MQ=255) gTCGAATGAACTTCATGTTCTATGttcgaccaatccttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccat < 1:17990‑M2/91‑68 (MQ=255) GTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCTAGCa > 2:192738‑M1/1‑91 (MQ=255) cGAATGATCTTCATGTTCTATGttcgaccaatccttattcctatacacaaattcctattacaacaatcacgactttgatagccccgccata < 2:3608222‑M2/91‑70 (MQ=255) cGAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccata < 1:3788723‑M2/91‑70 (MQ=255) gAAcatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaagcacgactttgatagccccgccataa < 2:895398‑M1/91‑89 (MQ=255) gAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccctaa < 2:2703893‑M2/91‑71 (MQ=255) gAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataa < 2:2549529‑M2/91‑71 (MQ=255) gAATGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataa < 2:2118264‑M2/91‑71 (MQ=255) aacatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataaa > 1:3388581‑M1/1‑2 (MQ=255) aaTGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataaa < 2:1589143‑M2/91‑72 (MQ=255) acatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataaaa < 2:2770446‑M1/91‑91 (MQ=255) acatagaacatgaagttcattcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataaaa < 1:614331‑M1/91‑91 (MQ=255) aTGAACTTCATGTTCTATGttcgaccaatcctttttcatatacaaaaattcctattacaacaatcacgactttgatagccccgccataaaa < 1:1056261‑M2/91‑73 (MQ=255) | AGTTGGATTGAATCCTAAATTTTATGGCGGGGCTATCAAAGTCGTGATTGTTGTAATAGGAATTTTTGTATATGAAAAAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATGTTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTT > USA300TCH1516_ALE/492633‑492813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |