mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | PAL053_S52_L007_R1_001.good.fq | 3,885,682 | 352,261,907 | 100.0% | 90.7 bases | 91 bases | 97.1% |
| errors | PAL053_S52_L007_R2_001.good.fq | 3,885,682 | 352,261,907 | 100.0% | 90.7 bases | 91 bases | 96.6% |
| total | 7,771,364 | 704,523,814 | 100.0% | 90.7 bases | 91 bases | 96.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 216.9 | 2.6 | 93.2% | Staphylococcus species strain strain. |
| coverage | distribution | CP000731 | 27,041 | 650.6 | 7.7 | 2.5% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| coverage | distribution | NC_012417 | 3,125 | 13678.7 | 44.8 | 4.3% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100000 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 5 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 971 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.063 |
| reference sequence | pr(no read start) |
|---|---|
| USA300TCH1516_ALE | 0.40881 |
| CP000731 | 0.13047 |
| NC_012417 | 0.02657 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:42:09 25 May 2019 | 07:43:39 25 May 2019 | 1 minute 30 seconds |
| Read alignment to reference genome | 07:43:39 25 May 2019 | 07:54:01 25 May 2019 | 10 minutes 22 seconds |
| Preprocessing alignments for candidate junction identification | 07:54:01 25 May 2019 | 07:55:32 25 May 2019 | 1 minute 31 seconds |
| Preliminary analysis of coverage distribution | 07:55:32 25 May 2019 | 07:57:54 25 May 2019 | 2 minutes 22 seconds |
| Identifying junction candidates | 07:57:54 25 May 2019 | 07:58:55 25 May 2019 | 1 minute 1 second |
| Re-alignment to junction candidates | 07:58:55 25 May 2019 | 08:03:46 25 May 2019 | 4 minutes 51 seconds |
| Resolving best read alignments | 08:03:46 25 May 2019 | 08:06:15 25 May 2019 | 2 minutes 29 seconds |
| Creating BAM files | 08:06:15 25 May 2019 | 08:08:20 25 May 2019 | 2 minutes 5 seconds |
| Tabulating error counts | 08:08:20 25 May 2019 | 08:09:23 25 May 2019 | 1 minute 3 seconds |
| Re-calibrating base error rates | 08:09:23 25 May 2019 | 08:09:25 25 May 2019 | 2 seconds |
| Examining read alignment evidence | 08:09:25 25 May 2019 | 08:50:16 25 May 2019 | 40 minutes 51 seconds |
| Polymorphism statistics | 08:50:16 25 May 2019 | 08:50:19 25 May 2019 | 3 seconds |
| Output | 08:50:19 25 May 2019 | 08:52:37 25 May 2019 | 2 minutes 18 seconds |
| Total | 1 hour 10 minutes 28 seconds | ||
