New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 1662000 =183 (1.040)28 (0.180) 17/156 12.8 15.1% intergenic (‑87/+68) USA300HOU_RS08275/rpmG2_2 5‑formyltetrahydrofolate cyclo‑ligase/50S ribosomal protein L33 2
?USA300TCH1516_ALE 1662039 = 157 (1.030)intergenic (‑126/+29) USA300HOU_RS08275/rpmG2_2 5‑formyltetrahydrofolate cyclo‑ligase/50S ribosomal protein L33 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/1662101‑1662000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttcacctttttTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTCA  >  USA300TCH1516_ALE/1662039‑1662128
                                                                                                                                                                                                
AAACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGT                                                                                                       <  2:1873536/91‑1
AAACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGT                                                                                                       >  2:1469705/1‑91
AAACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGT                                                                                                       >  2:1063753/1‑91
 AACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTT                                                                                                      <  1:2223061/91‑1
  ACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTT                                                                                                     <  2:2311614/91‑1
   CAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTC                                                                                                    <  2:1772921/91‑1
   CAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTC                                                                                                    <  2:1717154/91‑1
   CAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTC                                                                                                    <  1:2803363/91‑1
    AAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCA                                                                                                   <  1:1840062/91‑1
     AACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCAC                                                                                                  >  2:1686212/1‑91
     AACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCAC                                                                                                  >  1:717799/1‑91
     AACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCAC                                                                                                  >  1:502560/1‑91
      ACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACC                                                                                                 >  2:1116430/1‑91
      ACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACC                                                                                                 <  2:865084/91‑1
      ACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACC                                                                                                 <  2:864985/91‑1
      ACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACC                                                                                                 <  2:2388929/91‑1
      ACAAACTTTACACCATGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACC                                                                                                 <  2:1930450/91‑1
       CAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCT                                                                                                >  2:624179/1‑91
       CAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCT                                                                                                >  2:2679982/1‑91
       CAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCT                                                                                                >  2:2490204/1‑91
       CAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCT                                                                                                >  2:1381285/1‑91
       CAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCT                                                                                                >  1:705837/1‑91
        AAACTTTACGCCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTT                                                                                               <  1:1109078/91‑1
        AAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTT                                                                                               >  1:2981279/1‑91
        AAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTT                                                                                               >  2:1196254/1‑91
        AAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATGAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTT                                                                                               <  1:1109091/91‑1
         AACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTT                                                                                              <  2:1553808/91‑1
         AACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTT                                                                                              <  2:1553706/91‑1
          ACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTT                                                                                             >  1:1465537/1‑91
          ACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTT                                                                                             <  1:599726/91‑1
           CTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTT                                                                                            <  1:2222340/91‑1
           CTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTT                                                                                            >  2:1597153/1‑91
             TTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTA                                                                                          >  1:3018975/1‑91
                             AATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTT                                                                          >  2:2553780/1‑91
                                     TTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAA                                                                  >  1:175685/1‑91
                                              AATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTAATTGTTAATTTAAATTTAATTTTAATGATTATTT                                                         >  1:819028/1‑91
                                                      TTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCAC                                                 >  1:2574880/1‑91
                                                        AACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGG                                               >  2:398823/1‑91
                                                        AACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTAATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGG                                               >  1:739075/1‑91
                                                        AACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTAATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGG                                               >  1:736443/1‑91
                                                                AAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGT                                       >  2:1866469/1‑91
                                                                   AGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTG                                    >  2:2917097/1‑91
                                                                     GTGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTT                                  >  2:1271280/1‑91
                                                                      TGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTT                                 >  1:2762845/1‑91
                                                                      TGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTT                                 >  1:3020568/1‑91
                                                                      TGAAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTT                                 >  2:1717410/1‑91
                                                                        AAATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGT                               >  2:2093278/1‑91
                                                                          ATCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTT                             >  2:1442804/1‑91
                                                                           TCTCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTT                            >  1:1431710/1‑91
                                                                            CTCAAAATAAGAGGTTTCACCTTTTTAATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTC                           >  1:797871/1‑91
                                                                             TCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCA                          >  2:2210862/1‑91
                                                                             TCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCA                          >  2:2210643/1‑91
                                                                             TCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCA                          >  1:410265/1‑91
                                                                             TCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCA                          >  2:40088/1‑91
                                                                             TCAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCA                          >  1:2315645/1‑91
                                                                              CAAAATAAGAGGTTTCACCTTTTTTATTGTTCATTTAAATTTAATCATAATGATTATATTGTTTCACGATGTAAAGTTTGTTTGTTTTCAC                         >  2:1018305/1‑91
                                                                              CAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCAC                         >  1:604238/1‑91
                                                                              CAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCAC                         >  1:797618/1‑91
                                                                              CAAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCAC                         >  1:2148475/1‑91
                                                                               AAAATAAGAGGTTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACG                        >  1:738805/1‑91
                                                                                          TTTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCGCGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATT             <  1:2600233/91‑1
                                                                                          TTTCACCTTTTGTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACTGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATT             >  2:41644/1‑91
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGATCAGAATTT            >  2:2043401/1‑91
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTT            <  1:1527572/91‑1
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTT            <  1:1218316/91‑1
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTT            <  1:657765/91‑1
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTT            >  1:890268/1‑91
                                                                                           TTCACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTT            >  1:1374951/1‑91
                                                                                             CACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCT          <  2:3084233/91‑1
                                                                                             CACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCT          <  1:2093278/91‑1
                                                                                              ACCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTT         >  2:1487892/1‑91
                                                                                               CCTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTC        >  2:2163119/1‑91
                                                                                                CTTTTTTATTGTTAATTTAAATTTAATTTTACTGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTCTTCACGTGAACAGAATTTCTTCA       >  2:2364017/1‑91
                                                                                                CTTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCA       <  1:974710/91‑1
                                                                                                 TTTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCAT      >  2:1874551/1‑91
                                                                                                  TTTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATT     >  1:1766880/1‑91
                                                                                                   TTTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTT    <  1:2282146/91‑1
                                                                                                    TTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTC   >  2:2761167/1‑91
                                                                                                    TTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTC   >  2:2761177/1‑91
                                                                                                    TTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTC   >  2:2921499/1‑91
                                                                                                    TTTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTC   >  2:2921666/1‑91
                                                                                                     TTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTCA  <  1:421761/91‑1
                                                                                                                                                                                                
AAACAAACAAACTTTACACCGTGAAACAAAATAATCATTAAAATTAAATTTAAATTAACAATAAAAAAGGTGAAATCTCAAAATAAGAGGTTTCACCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/1662101‑1662000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttcacctttttTATTGTTAATTTAAATTTAATTTTAATGATTATTTTGTTTCACGGTGTAAAGTTTGTTTGTTTTCACGTGAACAGAATTTCTTCATTTCA  >  USA300TCH1516_ALE/1662039‑1662128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.