New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 622825	72 (0.830)	3 (0.040)	3/278	7.6	4.2%	coding (3388/3465 nt)	sdrE	Serine‑aspartate repeat‑containing protein E
	?	USA300TCH1516_ALE	= 622828	70 (0.860)					coding (3391/3465 nt)	sdrE	Serine‑aspartate repeat‑containing protein E
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACTCAGACAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCATATAAA  >  USA300TCH1516_ALE/622681‑622976                                                                                                                                                    |                                                                                                                                                     gactcagacAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAAc                                                                                                                                                     <  2:850513/149‑1 (MQ=255)  actcagacAGTGAATCAGACAGCCATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACg                                                                                                                                                    <  1:784981/149‑1 (MQ=255)   ctcagacAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGt                                                                                                                                                   <  2:864793/149‑1 (MQ=255)   ctcagacAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGt                                                                                                                                                   >  1:77368/1‑149 (MQ=255)   ctcagacAGGGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGt                                                                                                                                                   <  2:497698/149‑1 (MQ=255)     cagacAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTa                                                                                                                                                 <  2:298064/149‑1 (MQ=255)         cAGGGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTtg                                                                                                                                             <  2:107618/149‑1 (MQ=255)                 ctgACATCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGatta                                                                                                                                     <  1:909228/147‑1 (MQ=255)                  aGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGattat                                                                                                                                    <  2:643912/149‑1 (MQ=255)                    aCATCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGTCTCAAAGAACGCAACGTTATTTGGTGGATTAttt                                                                                                                                  <  1:449961/149‑1 (MQ=255)                    aCAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTAttt                                                                                                                                  <  1:887432/149‑1 (MQ=255)                       gCGATTCAGACTCGGATGCAGTAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTgca                                                                                                                               <  1:558995/149‑1 (MQ=255)                          aTTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACCACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTgcagca                                                                                                                            <  1:33233/149‑1 (MQ=255)                          aTTCAGACGCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAATACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTgcagca                                                                                                                            <  1:175091/149‑1 (MQ=255)                           ttCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCAt                                                                                                                           <  2:627038/149‑1 (MQ=255)                                aCTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGt                                                                                                                      >  1:901030/1‑149 (MQ=255)                                 cTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGtt                                                                                                                     >  1:276956/1‑149 (MQ=255)                                    ggATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCat                                                                                                                  <  2:315729/149‑1 (MQ=255)                                    ggATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCat                                                                                                                  <  2:535603/149‑1 (MQ=255)                                                   tACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTTTTGTTATTCGgtc                                                                                                   >  1:269219/1‑149 (MQ=255)                                                   tACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCgggc                                                                                                   >  1:187155/1‑147 (MQ=255)                                                       ccTGTTAACCCAATGAGTACTACTAAAGCCCACCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTTTTATTCGGtcgtcg                                                                                               <  2:180728/149‑1 (MQ=255)                                                             aaaCCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCaaaaa                                                                                         <  2:591732/149‑1 (MQ=255)                                                             aaaCCAATGAGGACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCaaaaa                                                                                         <  1:728594/149‑1 (MQ=255)                                                             aaaCAAAGGAATACTACTACAGACCACCACAATAAAGCAAAACCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCaaaaa                                                                                         <  1:907376/149‑1 (MQ=14)                                                               aCCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCaaaaaaa                                                                                       >  1:688314/1‑148 (MQ=255)                                                               aCCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAaaaac                                                                                       <  1:920082/149‑1 (MQ=255)                                                               aCCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAaaaac                                                                                       <  1:919798/149‑1 (MQ=255)                                                                ccAATTAGTACTACTACAGAACATCAGAGTAAAGCTAAAGCATTACCATATACAGGTAGTGAAAATAGCGGCTCAAATAACTGAACGTGATTTGGTGTTTTAGTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAaaaaca                                                                                      <  2:641348/149‑1 (MQ=14)                                                                  aaTGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATACCGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTACGCTCCATATTGGTCTTAGGCCGCAGCAAaaaacaaa                                                                                    >  2:32307/1‑149 (MQ=14)                                                                  aaTGAGTACTACTAAAGACCATCACAATAAAAATTCCTCAGTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAAAGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTGGCAAaaaacaaa                                                                                    >  1:222264/1‑149 (MQ=14)                                                                     gAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAaaacaaaaca                                                                                 <  1:306449/149‑1 (MQ=255)                                                                      actactaaCAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACTCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAaacaaaacaa                                                                                <  1:348550/147‑1 (MQ=255)                                                                      aGTACGACTAAATACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAAGACCGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCATCATTAGGTTCATTTTTGTTATTCGGTCGTCGCAAAAaacaaaacaa                                                                                <  1:598422/149‑1 (MQ=17)                                                                       gTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAGacaaaaaaaa                                                                               >  1:252614/1‑149 (MQ=255)                                                                         actactAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAaata                                                                             >  2:626591/1‑149 (MQ=255)                                                                         actactAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGATGGATTATTTGCAGCATTAGGTTCATTATTGTTATTGGGTCGTCGCAAAAAAAAAACCAcatc                                                                             >  2:228736/1‑148 (MQ=14)                                                                              taAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACa                                                                        >  1:313355/1‑149 (MQ=255)                                                                              aaaaGACCATCAAAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTTGCAAAAAACAAAACAAATAATACa                                                                        <  2:867629/148‑1 (MQ=255)                                                                                aaGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACaaa                                                                      >  2:877207/1‑148 (MQ=255)                                                                                 aGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAAAAATACAata                                                                     >  2:117536/1‑149 (MQ=255)                                                                                      aacaaaaTAAAGCAACAGCATTACCAGAAACAGGGAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGATTGAACTATAGAACAAAACAAATAATACAATATGAcc                                                                <  2:200349/144‑1 (MQ=17)                                                                                               aagcaaaagcaTTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTCGGTTCATTATTGGTAGTCGGTCGGCGCAAAAAACAAAAAAAATAATACAATATGACGCAGGTCCtt                                                       >  2:796842/1‑149 (MQ=17)                                                                                                        caTTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTgg                                              <  1:845277/149‑1 (MQ=255)                                                                                                        caTTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATAATTAACAGTATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCttg                                              <  1:918151/149‑3 (MQ=255)                                                                                                          ttACCAGAAACAGGTAGTGAAAATAATGGCTCAAATATCGCAAAGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtt                                            <  1:121739/149‑1 (MQ=255)                                                                                                          ttACCAGAAACAGGTAGTGAAAATAACGGCTCAACTACAGCAACGTTATGTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAAGAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtt                                            <  2:193406/149‑1 (MQ=21)                                                                                                          ttACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtt                                            <  1:306263/149‑1 (MQ=255)                                                                                                          ttACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTCCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtt                                            <  1:701998/149‑1 (MQ=255)                                                                                                              cAGAACTAGGGAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTCGATCATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtttttt                                        <  2:51058/149‑1 (MQ=21)                                                                                                              cAGAAACAGGTAGTGAAAATGACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtttttt                                        <  2:920739/149‑1 (MQ=255)                                                                                                              cAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtttttt                                        <  2:347630/149‑1 (MQ=255)                                                                                                              cAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtttttt                                        <  2:816065/149‑1 (MQ=255)                                                                                                              cAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGtttttt                                        <  1:492148/149‑1 (MQ=255)                                                                                                              cAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTACTTGTGACCTGGtttttt                                        >  2:564306/1‑149 (MQ=255)                                                                                                                 aaaCAGGTAGTGTAAATAACGGCTCAAATAACGCAACGCTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTtaa                                     >  2:566288/1‑148 (MQ=255)                                                                                                                 aaaCAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTtat                                     >  2:380579/1‑149 (MQ=255)                                                                                                                   aCAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAACACACAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATaa                                   <  1:409115/149‑1 (MQ=255)                                                                                                                       ggaGTGAAAATCACGGGTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTACTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTac                               <  2:563156/147‑1 (MQ=255)                                                                                                                       ggaGTGAAAAAAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATGTGCAGCATTAGGTTCATTATTGTTATTCGGTAGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTac                               <  2:325521/147‑1 (MQ=255)                                                                                                                       gTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTac                               <  1:615565/149‑1 (MQ=255)                                                                                                                       gTAGGGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATATGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTac                               <  2:502683/149‑1 (MQ=255)                                                                                                                        tAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTaca                              >  1:413927/1‑149 (MQ=255)                                                                                                                        tAGTGAAAATAACGGCTCAAAGAACGCAGCGTTATTTGGTTGATTATGTGCAGCATTAGGGTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTaca                              <  2:31497/149‑1 (MQ=25)                                                                                                                           tGAAAATAACGGCTCAAATAACGCCACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACAt                           >  1:554608/1‑149 (MQ=255)                                                                                                                              aaaTAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTTCACATGCa                        >  1:292106/1‑149 (MQ=255)                                                                                                                                  aaCGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTCTTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTTGCCTGGTTTTTTTATAATTTAACATgaattaa                    >  2:253930/1‑143 (MQ=21)                                                                                                                                  aaCGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGCTCGCCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCCATaa                    >  2:378089/1‑148 (MQ=255)                                                                                                                                           aagaaCGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTAtttt           <  2:185898/146‑1 (MQ=255)                                                                                                                                                cGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCata      >  2:344787/1‑149 (MQ=255)                                                                                                                                                cGCAACGTTATTTGGGGTGTTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCata      >  2:807769/1‑149 (MQ=255)                                                                                                                                                 gCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCatat     >  2:497023/1‑149 (MQ=255)                                                                                                                                                  cAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTCTTCAAATGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGGTTTTTTATAATTACACATGCAATAGATGTATTTTTCAtata    >  1:490620/1‑149 (MQ=255)                                                                                                                                                   aaCGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTTCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCATataa   <  1:406971/149‑1 (MQ=255)                                                                                                                                                    aCGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCATataaa  >  1:560827/1‑149 (MQ=255)                                                                                                                                                    aCGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATATTTCAGataaa  >  1:703714/1‑149 (MQ=255)                                                                                                                                                    aCGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGATTTTTTATAATTACACATGCAATAGATGTATTTTTCATataaa  >  2:123920/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GACTCAGACAGTGATTCAGACAGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAACAAATAATACAATATGACCCAGGTCCTTGTGGCCTGGTTTTTTTATAATTACACATGCAATAGATGTATTTTTCATATAAA  >  USA300TCH1516_ALE/622681‑622976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.