New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	52489 =	126 (1.040)	33 (0.310)	7/250	6.7	25.0%	intergenic (+99/‑574)	USA300HOU_RS00215/USA300HOU_RS00220	hypothetical protein/hypothetical protein
	?	USA300TCH1516_ALE	= 52983	86 (0.800)					intergenic (+593/‑80)	USA300HOU_RS00215/USA300HOU_RS00220	hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.

TGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGATA  >  USA300TCH1516_ALE/52849‑53123                                                                                                                                       |                                                                                                                                             tGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATGATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGCAATCATCGTAGTAATCGCGCGAAAATcg                                                                                                                                        <  1:881736/141‑1 (MQ=17) tGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAAGCGCGTGAAATAATCAGAGGAATCGTTTGAAATCAACCTAGTAATCGCGCGAAAATcg                                                                                                                                        <  1:328128/141‑1 (MQ=12)          cGTAGTAATCGCGCGCAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATa                                                                                                                               >  1:1019534/1‑141 (MQ=17)          cGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATAGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAGATCATCGTAGTAATCGAGCGAAAATAGCGTGaaaga                                                                                                                               >  2:1206672/1‑139 (MQ=255)             agtaCTCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATGGTACCAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTa                                                                                                                            >  1:933410/1‑141 (MQ=255)              gtaATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATAAGAGGAATCGTTTGAAAAAATCGTAGTAATCGCGCGAAAATGGCGTGAAgtagtaa                                                                                                                           >  2:1274505/1‑140 (MQ=11)                aaTCGCGCGAAAATCACGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATgg                                                                                                                         <  1:1064131/141‑1 (MQ=14)                 aTCCCGCGAAAATCGCGTGAAATTATCAGAGGAAGTGTTTGAAATCGTCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGt                                                                                                                        <  1:1309033/141‑1 (MQ=11)                   cgcgcgAAAATCTCGTGAAATAACCAGAGGAATCGTCCGGCTCCATTGTACTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAg                                                                                                                      <  2:1314136/141‑1 (MQ=11)                    gcgcgAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGa                                                                                                                     <  2:130749/141‑1 (MQ=40)                        gAAAAGCGCTTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGt                                                                                                                 <  1:619451/141‑1 (MQ=255)                          aaagcccgTGAAATAATCAGAGGAAGCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGttt                                                                                                               <  1:1055891/135‑1 (MQ=255)                          aaaTCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGttt                                                                                                               <  1:635509/141‑1 (MQ=255)                          aaaTCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGttt                                                                                                               <  1:132513/141‑1 (MQ=255)                          aaaTCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGttt                                                                                                               <  1:422783/141‑1 (MQ=255)                          aaaTCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGttt                                                                                                               <  1:410715/141‑1 (MQ=255)                             tCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGGGCAATACTGTGGTAGACGGTTTGAg                                                                                                            >  1:64917/1‑141 (MQ=255)                               gcgTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATcg                                                                                                                                        <  1:971063/111‑1 (MQ=11)                                cgTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGCATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACGATGGTAGACGGTTTGAGTaa                                                                                                         >  1:739606/1‑141 (MQ=255)                                  tggaataacCATTGGACTCGTTAAAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAAt                                                                                                       <  1:1020043/138‑1 (MQ=17)                                  tGAAATAATCAGGGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAAt                                                                                                       <  1:72667/141‑1 (MQ=255)                                    aaaTAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTa                                                                                                     <  1:230963/141‑1 (MQ=255)                                    aaaTAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTa                                                                                                     <  2:266139/141‑1 (MQ=255)                                     aataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAAGACTGTGGGAGACGGTTTGAGTAAATTaa                                                                                                    >  1:616149/1‑141 (MQ=255)                                      ataatCTGAGGAATCGTTTGGAATCATCGGAGTAATCGCGCGAAAATCGTGTGAAATAATCAGAGGAATCGTTTGACATCATAGTAGTAATCGCGCGTAAATCGCGTGATATAGTATGTTAGACGGTTTTAGTAAATTAAt                                                                                                   <  2:603222/141‑1 (MQ=14)                                        aatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATgg                                                                                                 <  1:233531/141‑1 (MQ=255)                                        aatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATgg                                                                                                 <  1:1366181/141‑1 (MQ=255)                                        aatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATgg                                                                                                 <  1:841612/141‑1 (MQ=255)                                               ggAATCGTTTGAAATCATCGTAGTAGTCGCGCGAAAATCGCGTGAAATAATCAGAGGGATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGGAATACGATGGTAGACGGGTTGGGTAAATTAGTGGAGGAttt                                                                                          >  1:50502/1‑141 (MQ=17)                                               ggAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGACTCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTCGCCGGTTTGGGTAAATTAACGGAGTAttt                                                                                          >  1:736709/1‑141 (MQ=17)                                                     gTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAatat                                                                                    >  2:508196/1‑141 (MQ=255)                                                             tcatcGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGtt                                                                            <  1:986630/141‑1 (MQ=255)                                                              catcGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGttt                                                                           <  1:1332440/141‑1 (MQ=255)                                                              catcGTAGTAATCGCGCGAAAATCACGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGttt                                                                           <  2:852257/141‑1 (MQ=255)                                                               atcGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTACTTTAATATTTATGGTTTg                                                                          >  2:417682/1‑141 (MQ=255)                                                               atcGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACGATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTg                                                                          >  1:50654/1‑141 (MQ=255)                                                                 cGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGt                                                                                                                        >  2:120882/1‑93 (MQ=38)                                                                 cGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGt                                                                                                                        <  1:120882/93‑1 (MQ=38)                                                                  gtagtaATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTggag                                                                       <  1:362616/141‑1 (MQ=255)                                                                  gtagcaATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTggag                                                                       <  1:897194/141‑1 (MQ=255)                                                                    agtcATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATATTTTAGGCGTCCGTTTGAGTACATTAATTGAGGATTTTAATATTTATGGTTTggagga                                                                     <  1:529817/141‑1 (MQ=17)                                                                     gtcaTCGCGCGAACATCGCTTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGGAGACGGTTTAAGTAAATTAATGGAGTATTTTAATAGTTATGGTTTGGAGGaa                                                                    <  2:154677/138‑1 (MQ=21)                                                                     gtaATCGCGCGAAAATCGCGTGAAATAATCTGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGATTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGaa                                                                    <  2:870831/141‑1 (MQ=255)                                                                              cgAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGc                                                           >  2:1103591/1‑141 (MQ=255)                                                                                   aTCGCGTGAAATAATCAGAGGAATCGTTTGAAAACATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATaga                                                      <  1:1010200/141‑1 (MQ=255)                                                                                    tCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATagag                                                     <  1:1133135/141‑1 (MQ=255)                                                                                    tCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATagag                                                     <  1:508196/141‑1 (MQ=255)                                                                                      cggTGAAATAATCAGAAGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAAGATTTATGGTTTGGAGGAATTGCGTTGCATAGAGaa                                                   <  2:1053265/139‑1 (MQ=255)                                                                                        cggAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAg                                                 <  1:1274505/139‑1 (MQ=255)                                                                                         tGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGt                                                <  1:281650/141‑1 (MQ=255)                                                                                         ggAAAAAATCAGAAGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGt                                                <  1:368515/140‑1 (MQ=255)                                                                                          gAAATAATACGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAACTCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAAGATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTa                                               <  2:585254/141‑1 (MQ=255)                                                                                            aataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTaaa                                             <  2:933410/141‑1 (MQ=255)                                                                                             ataatCAGAGGAATCGTTTGAAATCATCGTTGTAATGGCGCGATACGCGCTGGATATACAAGGGCAGACGGTATGAGTAAGGTAATGGAGTACGTTAATATTGNTGGTTTGGAGGAATTGCGTTGCATAGTGCAAGTaaac                                            >  2:83444/1‑140 (MQ=19)                                                                                             ataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAGGCGCGTGAAATAGTCTGGTAGAAGGTATGAGTAAATGAATGGAGAATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTaaaa                                            >  2:341637/1‑140 (MQ=25)                                                                                             ataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAat                                            <  1:395277/141‑1 (MQ=255)                                                                                             ataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAat                                            >  2:826341/1‑141 (MQ=255)                                                                                             ataatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAat                                            >  2:1080332/1‑141 (MQ=255)                                                                                               aatCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAatat                                          <  1:1307904/141‑1 (MQ=255)                                                                                                 tCAGAGGAATCGTTTGGAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAatatat                                        >  1:700581/1‑141 (MQ=255)                                                                                                 tCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAatatat                                        <  1:484669/141‑1 (MQ=255)                                                                                                 tCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAaaatat                                        >  1:469533/1‑141 (MQ=255)                                                                                                   agagGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATag                                      <  1:295687/141‑1 (MQ=255)                                                                                                   agagGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATag                                      <  1:907808/141‑1 (MQ=255)                                                                                                   agagGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATag                                      <  1:649841/141‑1 (MQ=255)                                                                                                     agGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATagag                                    <  1:1266985/141‑1 (MQ=255)                                                                                                     agGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATagag                                    <  1:557014/141‑1 (MQ=255)                                                                                                        aaTCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGtat                                 <  1:1103591/141‑1 (MQ=255)                                                                                                         aTCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGtata                                >  1:763095/1‑141 (MQ=255)                                                                                                          tCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATaa                               >  2:219837/1‑141 (MQ=255)                                                                                                          tCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATaa                               >  2:1100125/1‑141 (MQ=255)                                                                                                          tCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAAGTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGGATaa                               >  2:342466/1‑141 (MQ=255)                                                                                                            gTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGa                             <  1:225114/141‑1 (MQ=255)                                                                                                            gTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGa                             <  1:81849/141‑1 (MQ=255)                                                                                                                gAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTACACGGTTTGAGTAAATTAATGGAGTATTTTAATATATCTGGTTTGGAGGAATTGCGTTGCATTGAGAAAGTAAATATATAGAGTATAAGaaagc                         >  2:891639/1‑139 (MQ=255)                                                                                                                    tcatcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGa                     <  2:572814/141‑1 (MQ=255)                                                                                                                    tcatcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGa                     <  1:1247905/141‑1 (MQ=255)                                                                                                                    tcatcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGa                     <  1:916393/141‑1 (MQ=255)                                                                                                                    tcatcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATAGATAGCGTATAAGAAATCACGa                     <  1:698622/141‑1 (MQ=255)                                                                                                                      atcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGaaa                   >  2:429379/1‑141 (MQ=255)                                                                                                                      atcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTCGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGaaa                   >  2:1201276/1‑141 (MQ=255)                                                                                                                       tcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGaaaa                  <  2:438222/141‑1 (MQ=255)                                                                                                                       tcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGaaaa                  <  2:375923/141‑1 (MQ=255)                                                                                                                       tcGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGaaaa                  <  2:985442/141‑1 (MQ=255)                                                                                                                         gtagtaATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTTCGTTGCATAGAGAAAGTAAATATATAGAGTAGAAGAAATCAAGAAAAgg                >  1:852745/1‑141 (MQ=255)                                                                                                                                  gcgcgAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTAGAAGAAATCACGAAAAGGATTATCTaa       >  2:1076102/1‑141 (MQ=255)                                                                                                                                      gAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGat   <  2:562866/141‑1 (MQ=255)                                                                                                                                       aaaaTCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATACGAAATCCCGACCAGGATTATCTAATGata  <  2:1361081/141‑1 (MQ=255)                                                                                                                                       aaaaTCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGata  >  1:979378/1‑141 (MQ=255)                                                                                                                                       aaaaTCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGata  >  1:1339228/1‑141 (MQ=255)                                                                                                                                       aaaaTCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGata  >  2:87850/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                             TGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATAATCAGAGGAATCGTTTGAAATCATCGTAGTAATCGCGCGAAAATCGCGTGAAATACTATGGTAGACGGTTTGAGTAAATTAATGGAGTATTTTAATATTTATGGTTTGGAGGAATTGCGTTGCATAGAGAAAGTAAATATATAGAGTATAAGAAATCACGAAAAGGATTATCTAATGATA  >  USA300TCH1516_ALE/52849‑53123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.