New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2545705 =160 (1.140)5 (0.040) 5/234 NT 3.6% intergenic (+6/+46) hlgB/USA300HOU_RS13110 Gamma‑hemolysin component B/hypothetical protein
?USA300TCH1516_ALE 2545745 = 122 (0.960)intergenic (+46/+6) hlgB/USA300HOU_RS13110 Gamma‑hemolysin component B/hypothetical protein
Rejected: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

TGGAATGGCTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGTTTAACA  >  USA300TCH1516_ALE/2545565‑2545845
                                                                                                                                            |                                                                                                                                             
tGGAATGGCTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTa                                                                                                                                               >  1:586802/1‑141 (MQ=255)
tGGAATGGCTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTa                                                                                                                                               >  1:1090556/1‑141 (MQ=255)
 attatctattattttaaatttacctgtgtgggtagtgttgttaaacgcgattccaggcgtaatattcactttaattgtagctattcctttatatcacacataaagaaaaagaatggcagtattactaagataataaatcaa                                                                                                                                              >  2:209304‑M1/140‑141 (MQ=255)
  ttatctattattttaaatttacctgtgtgggtagtgttgttaaacgcgattccaggcgtaatattcactttaattgtagctattcctttatatctcacattgagaaaaagaatggcagtattactaagataataaatcaaa                                                                                                                                             >  1:151696‑M1/139‑141 (MQ=255)
  ttatctattattttaaatttacctgtgtgggtagtgttgttaaacgcgattccaggcgtaatattcactttaattgtagctattcctttatatctcacattgagaaaaagaatggcagtattactaagataataaatcaaa                                                                                                                                             <  1:386908‑M1/3‑1 (MQ=255)
        cTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACgg                                                                                                                                       <  3:399394/141‑1 (MQ=255)
        cTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACgg                                                                                                                                       <  1:216789/141‑1 (MQ=255)
          tCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTc                                                                                                                                     >  3:236119/1‑141 (MQ=255)
           cTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCg                                                                                                                                    >  2:960684/1‑141 (MQ=255)
           attttaaatttacctgtgtgggtagtgttgttaaacgcgattccaggcgtaatattcactttaatcgtagctattcctttatatctcacattgagaaaaagaatggcagtattactaagataataaatcAAAAcacggacg                                                                                                                                    <  1:845118‑M1/12‑4 (MQ=255)
              cTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCa                                                                                                                                 <  2:1170102/141‑1 (MQ=255)
                 ggCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACa                                                                                                                              >  4:312460/1‑141 (MQ=255)
                 ggCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACa                                                                                                                              >  2:620523/1‑141 (MQ=255)
                  gCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAg                                                                                                                             <  1:496892/141‑1 (MQ=255)
                  gCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAg                                                                                                                             >  2:770860/1‑141 (MQ=255)
                  gCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAg                                                                                                                             >  2:1123457/1‑141 (MQ=255)
                  gCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAg                                                                                                                             >  2:1018362/1‑141 (MQ=255)
                     tacctgtgtgggtagtgttgttaaacgcgattccaggcgtaatattcactttaattgtagctattcctttatatctcacattgagaaaaagaatggcagtattactaagataataaatcAAAACACGGTCGCCAACAGTaa                                                                                                                          >  4:74686‑M1/120‑141 (MQ=255)
                      gcgcAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAAt                                                                                                                         >  1:211161/1‑141 (MQ=255)
                       cgcAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAAtt                                                                                                                        >  4:346553/1‑141 (MQ=255)
                          aaaTTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATtgt‑g                                                                                                                    >  3:182011/1‑141 (MQ=255)
                          aaaTTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATtgt‑g                                                                                                                    >  1:622867/1‑141 (MQ=255)
                          aaaTTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATtgt‑g                                                                                                                    <  1:1061818/141‑1 (MQ=255)
                           aaTTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑ga                                                                                                                   >  1:402930/1‑141 (MQ=255)
                            aTTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑gac                                                                                                                  <  1:448691/141‑1 (MQ=255)
                               ataAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑gacgac                                                                                                               >  4:141717/1‑141 (MQ=255)
                                 aaaaaCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCg                                                                                                             <  3:162027/141‑1 (MQ=255)
                                  aaaaCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCgt                                                                                                            >  1:1002855/1‑141 (MQ=255)
                                   aaaCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCgtg                                                                                                           >  1:654822/1‑141 (MQ=255)
                                   aaaCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCgtg                                                                                                           <  2:474343/141‑1 (MQ=255)
                                   aaaCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCgtg                                                                                                           <  1:875126/141‑1 (MQ=255)
                                     aCTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAGATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGtt                                                                                                         <  1:229533/141‑1 (MQ=255)
                                        ttAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGTCCGTGTTTTg                                                                                                      <  2:93616/141‑1 (MQ=255)
                                          aaaaCTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGAt                                                                                                    <  1:86277/141‑1 (MQ=255)
                                          aaaaCTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGAt                                                                                                    <  1:968063/141‑1 (MQ=255)
                                           aaaCTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGAtt                                                                                                   >  1:1026548/1‑141 (MQ=255)
                                           aaaCTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGAtt                                                                                                   >  1:187217/1‑141 (MQ=255)
                                            aaCTAGAACATTCAAATCAACATAGGAAATTGATTGGGAAAATCACAAAGTGAAATTGTGAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGAgtt                                                                                                  >  2:113448/1‑141 (MQ=255)
                                                aGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATttatta                                                                                              >  3:79829/1‑141 (MQ=255)
                                                 gAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTtattat                                                                                             >  2:808469/1‑141 (MQ=255)
                                                     aTTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTAT‑GACGACCGTGTTTTGATTTATTATCTTa                                                                                         <  1:301941/141‑1 (MQ=255)
                                                          aaTCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAAt                                                                                    >  2:754033/1‑141 (MQ=255)
                                                              aaCATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTg                                                                                <  1:520463/141‑1 (MQ=255)
                                                                cATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGCAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGCTTTGATTTATTATCTTAGTTATACTGcc                                                                              >  2:253427/1‑141 (MQ=255)
                                                                  tatGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATCGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCAt                                                                            >  1:939414/1‑141 (MQ=255)
                                                                    tGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTc                                                                          <  2:169209/141‑1 (MQ=255)
                                                                     gAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCt                                                                         >  3:413767/1‑141 (MQ=255)
                                                                     gAAAATGATTGAGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCt                                                                         <  2:352229/141‑1 (MQ=255)
                                                                         ttgattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCttttt                                                                     <  4:122994/141‑1 (MQ=255)
                                                                         ttgattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCttttt                                                                     <  3:33584/141‑1 (MQ=255)
                                                                          tgattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTtc                                                                    <  4:269776/141‑1 (MQ=255)
                                                                          tgattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTtc                                                                    <  2:515685/141‑1 (MQ=255)
                                                                          tgattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTtc                                                                    <  3:445203/141‑1 (MQ=255)
                                                                           gattgGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTtct                                                                   <  3:94868/141‑1 (MQ=255)
                                                                              tgGGAAAATCACAGAGTGAAATTGTTAGATACGAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGCCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTCTTATCTTAGTAATACTGCCATTCTTTTTCTCaa                                                                >  2:432424/1‑141 (MQ=255)
                                                                               gggAAAATCACAAAGTGAAATTGGTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAAt                                                               >  4:417455/1‑141 (MQ=255)
                                                                                ggAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAAtg                                                              >  1:736611/1‑141 (MQ=255)
                                                                                   aactCACAAAGTGAAATTGTTAGATACAAAAGAACCTGAAAACAATAAATAGCTGGTATAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTga                                                           <  2:1113780/138‑1 (MQ=255)
                                                                                    aaTCACAAAGTGAAATTGTTAGATACAAAAGAATCTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTgag                                                          <  2:364931/141‑1 (MQ=255)
                                                                                    aaTCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTgag                                                          >  3:363676/1‑141 (MQ=255)
                                                                                     aTCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTgaga                                                         >  4:264424/1‑141 (MQ=255)
                                                                                     aTCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGACGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTgaga                                                         >  4:54812/1‑141 (MQ=255)
                                                                                         caAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGAtata                                                     <  1:996549/141‑1 (MQ=255)
                                                                                         caAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGAtata                                                     >  1:560879/1‑141 (MQ=255)
                                                                                         caAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAAGACTGCCATTCTTTTTCTCAATGTGAGAtata                                                     <  2:72203/141‑1 (MQ=255)
                                                                                          aaaGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATaa                                                    >  4:442040/1‑141 (MQ=255)
                                                                                          aaaGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATaa                                                    >  1:77012/1‑141 (MQ=255)
                                                                                          aaaGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATaa                                                    >  1:901985/1‑141 (MQ=255)
                                                                                            aGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAg                                                  <  2:187217/141‑1 (MQ=255)
                                                                                                 aaTTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATa                                             >  3:288081/1‑141 (MQ=255)
                                                                                                  aTTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAg                                            >  1:896773/1‑141 (MQ=255)
                                                                                                    tGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCt                                          <  3:85848/141‑1 (MQ=255)
                                                                                                    tGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCt                                          >  3:98945/1‑141 (MQ=255)
                                                                                                     gTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGTGATATAAAGGAATAGCtt                                         >  4:237906/1‑140 (MQ=255)
                                                                                                      ttAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTAc                                        <  2:304994/141‑1 (MQ=255)
                                                                                                          aTACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAAtt                                    <  3:319174/141‑1 (MQ=255)
                                                                                                                aaGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTg                              <  1:265837/141‑1 (MQ=255)
                                                                                                                 aGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGa                             >  4:394031/1‑141 (MQ=255)
                                                                                                                 aGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGa                             >  2:23287/1‑141 (MQ=255)
                                                                                                                  tggcagtattactaagataataaatcAAAACACGGTCGTCA‑CAATTACTGTTGGCGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAAt                                     >  1:171094‑M1/27‑132 (MQ=255)
                                                                                                                  gAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGaa                            >  1:665560/1‑141 (MQ=255)
                                                                                                                   aaaCTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAat                           <  4:20521/141‑1 (MQ=255)
                                                                                                                     aCTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAatat                         >  3:494594/1‑141 (MQ=255)
                                                                                                                     aCTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAatat                         >  1:82819/1‑141 (MQ=255)
                                                                                                                         aaaaCAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACg                     >  3:313467/1‑141 (MQ=255)
                                                                                                                         aaaaCAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACg                     >  3:24854/1‑141 (MQ=255)
                                                                                                                         aaaaCAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACg                     >  2:630533/1‑141 (MQ=255)
                                                                                                                         aaaaCAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATAGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACg                     >  2:874775/1‑141 (MQ=255)
                                                                                                                         aaaaCAATAAATAGCTAGGAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACg                     <  3:106607/141‑1 (MQ=255)
                                                                                                                            aCAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCt                  <  4:174123/141‑1 (MQ=255)
                                                                                                                               ataaataGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGa               <  2:1102753/141‑1 (MQ=255)
                                                                                                                                  aataGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATc            <  2:1029731/141‑1 (MQ=255)
                                                                                                                                  aataGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATc            >  1:833478/1‑141 (MQ=255)
                                                                                                                                    taGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTCTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATcgc          >  1:1150859/1‑141 (MQ=255)
                                                                                                                                      gCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGt        <  1:814815/141‑1 (MQ=255)
                                                                                                                                      gCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGt        >  2:764659/1‑141 (MQ=255)
                                                                                                                                       cTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGCTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGtt       >  3:33574/1‑141 (MQ=255)
                                                                                                                                        tAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGttt      >  2:1065970/1‑141 (MQ=255)
                                                                                                                                           tAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGTTTaac   <  2:484764/141‑1 (MQ=255)
                                                                                                                                            AAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGTTTaaca  <  2:882133‑M1/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TGGAATGGCTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTTAAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAAACTGAAAACAATAAATAGCTAGTAAAACACGGTCGCCAACAGTAATTGT‑GACGACCGTGTTTTGATTTATTATCTTAGTAATACTGCCATTCTTTTTCTCAATGTGAGATATAAAGGAATAGCTACAATTAAAGTGAATATTACGCCTGGAATCGCGTTTAACA  >  USA300TCH1516_ALE/2545565‑2545845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.