BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 2600866	90 (1.300)	3 (0.050)	3/238	NT	3.3%	intergenic (‑278/+589)	dapF/USA300HOU_RS13395	Diaminopimelate epimerase/hypothetical protein
*	?	USA300TCH1516_ALE	= 2600882	92 (1.450)	3 (0.050)	3/238	NT	3.3%	intergenic (‑294/+573)	dapF/USA300HOU_RS13395	Diaminopimelate epimerase/hypothetical protein
Rejected: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

TAATTCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGTTTTCACCTCAACT  >  USA300TCH1516_ALE/2600742‑2601021

tAATTCGCAAAAGCGAAAACCATGGCGCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGaa                                                                                                                                              <  1:77740/141‑1 (MQ=255)

 aaTTCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAa                                                                                                                                             <  2:148684‑M2/141‑2 (MQ=255)

  aTTCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAag                                                                                                                                            >  1:707507‑M2/1‑139 (MQ=255)

  aTTCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAag                                                                                                                                            <  1:755140‑M2/141‑3 (MQ=255)

   ttCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAagc                                                                                                                                           <  1:651345‑M2/141‑4 (MQ=255)

   atcGCAACAGAGAAAACACTGGCTCTTATGCAGTTGTTGCTAAGCAAGCACCTGTATTCCTCGAAAACTGTAATTTTTTAGAAGCTGAACCTAAAAGCTCTATCCATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAagc                                                                                                                                           >  2:211291‑M2/2‑138 (MQ=255)

     cGCAAAAGCGAAAACCATGGCACTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAagcat                                                                                                                                         <  2:573124‑M2/141‑6 (MQ=255)

         aaaGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAagcatgact                                                                                                                                     <  2:62130‑M2/141‑10 (MQ=255)

                aaaCCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACCTAGTAAt                                                                                                                              >  1:351936/1‑141 (MQ=255)

                    tattgCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGgt                                                                                                                          <  2:360578/137‑1 (MQ=255)

                    cATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATGGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGgt                                                                                                                          <  1:241414/141‑1 (MQ=255)

                    cATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGgt                                                                                                                          <  2:206203/141‑1 (MQ=255)

                            ttATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTAt                                                                                                                  <  2:229565/141‑1 (MQ=255)

                                gCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCaa‑a                                                                                                             <  1:148684/141‑1 (MQ=255)

                                gCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCaa‑a                                                                                                             <  2:579246/141‑1 (MQ=255)

                                  aGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AAt                                                                                                           <  1:170875/141‑1 (MQ=255)

                                   gttgttGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTTAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTTTCAA‑AATg                                                                                                          >  2:652081/1‑141 (MQ=255)

                                   gttgttGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATg                                                                                                          >  2:146467/1‑141 (MQ=255)

                                       ttgCGAAGGAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAc                                                                                                      <  2:643803/141‑1 (MQ=255)

                                       ttgCGAAGCAAGCAACTTTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCCCTGTGAACAGCATGACTTAGTAATCGGTGGTTGTATCAA‑AATGCAAc                                                                                                      <  2:764175/141‑1 (MQ=21)

                                       ttgCGAAGCAAGCAACTGTATTCCTCTAAAACTGTACTTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAc                                                                                                      >  2:198291/1‑141 (MQ=255)

                                        tgCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAcc                                                                                                     >  2:778994/1‑141 (MQ=255)

                                        tgCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAcc                                                                                                     >  1:758619/1‑141 (MQ=255)

                                        tgCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAcc                                                                                                     <  1:40283/141‑1 (MQ=255)

                                        tgCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAAcc                                                                                                     <  2:571560/141‑1 (MQ=255)

                                         gCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGCCTTAGTAATCGGTTCTTGTTTACA‑AATGCAACCg                                                                                                    >  2:354159/1‑141 (MQ=25)

                                         gCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCg                                                                                                    <  2:106805/141‑1 (MQ=255)

                                         gCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCg                                                                                                    >  2:514424/1‑141 (MQ=255)

                                          cGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTTTCCACTTTGCG‑GGTTagcatgacttacctaatagatagagctattaggttcagcttc                                                                                                    <  1:392057‑M2/141‑43 (MQ=255)

                                           gAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTa                                                                                                  <  1:479297/141‑1 (MQ=255)

                                                 agccaCTTTATTCCTCTAAAACCGTAATTTTTTAGAAGCTGACCCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGGGGAAAGGATGACTGAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCtt                                                                                            <  2:15775/141‑1 (MQ=255)

                                                  gcaaCTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAagcatgacttacctaatagatagagctattaggttcagcttctaaaaaat                                                                                            <  2:392057‑M2/141‑51 (MQ=255)

                                                  gcaaCTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTa                                                                                           >  1:47116/1‑141 (MQ=255)

                                                     aCTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCCA‑AATGCAACCGTAAACCTTAttt                                                                                        >  2:67247/1‑141 (MQ=255)

                                                      cTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTa                                                                                       >  1:43470/1‑141 (MQ=255)

                                                           ttCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGtt                                                                                  >  2:821094/1‑141 (MQ=255)

                                                            tCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTg                                                                                 <  1:23354/141‑1 (MQ=255)

                                                             ccTCTAAAACTGTAATTTTTTGGAAGCTGAACCTAATAGCTCTATCTATTACGTAAGTGATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGc                                                                                <  2:552818/141‑1 (MQ=255)

                                                             ccTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTACTCGGTTGTTGGATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGc                                                                                <  2:630223/141‑1 (MQ=255)

                                                             ccTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCGCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGTTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGc                                                                                <  2:137597/141‑1 (MQ=255)

                                                               tctAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGctt                                                                              <  1:351192/141‑1 (MQ=255)

                                                                   aaaCTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACT‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTtg                                                                          <  1:191407/141‑1 (MQ=255)

                                                                   aaaCTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGCAATCGTACGTTTTATCAA‑AATGCAACCGTAAACCTGATTTAAAGTTGCTTCTtg                                                                          >  1:689273/1‑141 (MQ=37)

                                                                       tGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGc                                                                      <  2:755140/141‑1 (MQ=255)

                                                                           aTTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTa                                                                  <  1:502708/141‑1 (MQ=255)

                                                                              ttttAGAAGCTGAACCCTATAGCTCTCTCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACaa                                                               <  1:513834/141‑1 (MQ=255)

                                                                               tttAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAg                                                              <  1:766701/141‑1 (MQ=255)

                                                                               tttAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAg                                                              <  1:810995/141‑1 (MQ=255)

                                                                               tttAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAg                                                              <  2:794281/141‑1 (MQ=255)

                                                                                ttAGAAGCTTAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACaaac                                                             >  2:405597/1‑139 (MQ=255)

                                                                                ttAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTGGCTTCTGGTGGCCCTACAAGc                                                             >  1:89488/1‑141 (MQ=255)

                                                                                  aGAAGCTGAACCTAATAGCTCTATCTATTAGGCAAGTCATGCTAACC‑CGCAAAGTGGAAagcatgacttacctaatagatagagctattaggttcagcttctaaaaaattacagttttagaggaatacagttgcttgcttc                                                            <  1:599734‑M2/141‑83 (MQ=255)

                                                                                   gAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGaa                                                          >  1:147860/1‑141 (MQ=255)

                                                                                     aGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAAAC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGAAACCGTAAACCTTATTTAGAGTTATAGCTTTGGGCTCTACAAGCGAATg                                                        >  1:687837/1‑141 (MQ=38)

                                                                                      gCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGc                                                       <  1:514424/141‑1 (MQ=255)

                                                                                       cTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTTGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCa                                                      >  1:706707/1‑141 (MQ=255)

                                                                                       cTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGcg                                                      >  1:746419/1‑140 (MQ=255)

                                                                                       cTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGCAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCa                                                      <  1:574574/141‑1 (MQ=255)

                                                                                        tGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTTAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAg                                                     <  2:720434/141‑1 (MQ=255)

                                                                                        tGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGATCTAAACGCGAATGCAg                                                     <  2:701470/141‑1 (MQ=255)

                                                                                        tGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGACTGACGCATCGAGCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAg                                                     <  1:608893/141‑1 (MQ=38)

                                                                                        tGAACCTAATAGCTCTATCCATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGGTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAg                                                     <  2:362649/141‑1 (MQ=255)

                                                                                        cgAACCGAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCACCCGTCAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAg                                                     <  2:629872/140‑1 (MQ=255)

                                                                                            ccTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGt                                                 <  1:66689/141‑1 (MQ=255)

                                                                                               aagaGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCg                                              >  2:728724/4‑141 (MQ=255)

                                                                                                  aGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTa                                           <  1:62130/141‑1 (MQ=255)

                                                                                                    ctctATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAAc                                         <  1:342883/141‑1 (MQ=255)

                                                                                                       tatctatTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGAATTCGAATTCTTTTGTTGTATGAT‑TAGTGTAGCGTAAACCTAATTTAAAGGTGGTGCTTTTTGCTCTACAAGCGAATGCAGTTGTCCGTTAACTAc                                      >  1:612600/1‑141 (MQ=255)

                                                                                                           tatTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGGTCTACAAGCGAATGCAGGTGTCCGTTAACTACATTc                                  >  2:23643/1‑141 (MQ=255)

                                                                                                           tatTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCAGTTAACTACATTc                                  >  2:762864/1‑141 (MQ=255)

                                                                                                            atTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTCATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTACGTTAACTACATTCa                                 >  2:1707/1‑141 (MQ=255)

                                                                                                            atTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCa                                 >  1:700383/1‑141 (MQ=255)

                                                                                                                 gTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAg                            <  2:400646/141‑1 (MQ=255)

                                                                                                                    aGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATg                         >  1:70010/1‑141 (MQ=255)

                                                                                                                     gTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGc                        >  1:288209/1‑141 (MQ=255)

                                                                                                                      tCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCt                       <  2:651345/141‑1 (MQ=255)

                                                                                                                        atcctgaCC‑CGCAAAGTGGAAAGCATGACGTAGTAATCGGATGTTGCATCAA‑AATGCAACCGTAAACCTAATTTAACGTTGCCTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCttt                     <  2:125738/135‑1 (MQ=255)

                                                                                                                        aTGCTAACC‑CGCAAAGTGGAATGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCttt                     >  2:809763/1‑141 (MQ=255)

                                                                                                                        aTGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCttt                     <  2:135462/141‑1 (MQ=255)

                                                                                                                        aTGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCttt                     >  2:713687/1‑141 (MQ=255)

                                                                                                                        aTGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCttt                     >  1:773879/1‑141 (MQ=255)

                                                                                                                        aTGCGAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCCCTTAGATGCttt                     >  2:811596/1‑141 (MQ=255)

                                                                                                                         tGCTAATC‑CGCAAGGTTGAGCTCATGACTGAGTGATCGTTTGTTGTATCAA‑AGTGCAACCGTAAACTTTATTTAAATTTGCTTCTTGTGGCTCTACAAGCGAAGGCAGTTGTCCGTTAACTACATTCACTTAGATGCtttt                    <  2:376571/141‑1 (MQ=255)

                                                                                                                         tGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCtttt                    >  1:87805/1‑141 (MQ=255)

                                                                                                                         tGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCtttt                    >  1:482894/1‑141 (MQ=255)

                                                                                                                         tGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAATGGTTTTTTAACGACATTCACTTAGATGCtttt                    <  1:405597/141‑1 (MQ=255)

                                                                                                                          gCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTa                   <  1:510538/141‑1 (MQ=255)

                                                                                                                           cTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTAc                  >  2:557649/1‑141 (MQ=255)

                                                                                                                           cTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTAc                  <  1:625528/141‑1 (MQ=255)

                                                                                                                                c‑cGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAATGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGttt             <  2:392789/141‑1 (MQ=255)

                                                                                                                                c‑cGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGttt             <  2:546014/141‑1 (MQ=255)

                                                                                                                                c‑cGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGttt             <  1:644706/141‑1 (MQ=255)

                                                                                                                                c‑cGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGttt             <  1:131971/141‑1 (MQ=255)

                                                                                                                                c‑cGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGGTAACTACATTCACTTAGATGCTTTTACTGttt             <  1:198291/141‑1 (MQ=255)

                                                                                                                                  cGCAAATGGGAAAGCATGACTTAGTAATCGGTTGGTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGtttt            <  2:191709/141‑1 (MQ=255)

                                                                                                                                   gCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AAGGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGTTTTc           >  2:448176/1‑141 (MQ=255)

                                                                                                                                       aGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCCCTTAGATGCTTTTACTGTTTTCACCt       >  2:344959/1‑141 (MQ=255)

                                                                                                                                           gAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGTTTTCACCTCAAc   <  2:613742/141‑1 (MQ=255)

                                                                                                                                           gAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGGTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTGCTGTTTTCACCTCAAc   >  1:177331/1‑141 (MQ=255)

                                                                                                                                             aaGCCTGACTTAGGTATCGGTTGTTGTATCAATAATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGTTTTCACCTCAACt  <  1:560661/141‑1 (MQ=255)

TAATTCGCAAAAGCGAAAACCATGGCTCTTATGCAGTTGTTGCGAAGCAAGCAACTGTATTCCTCTAAAACTGTAATTTTTTAGAAGCTGAACCTAATAGCTCTATCTATTAGGTAAGTCATGCTAACC‑CGCAAAGTGGAAAGCATGACTTAGTAATCGGTTGTTGTATCAA‑AATGCAACCGTAAACCTTATTTAAAGTTGCTTCTTGTGGCTCTACAAGCGAATGCAGTTGTCCGTTAACTACATTCACTTAGATGCTTTTACTGTTTTCACCTCAACT  >  USA300TCH1516_ALE/2600742‑2601021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.