New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2328479 =128 (1.250)4 (0.040) 3/288 8.8 3.2% coding (1185/1713 nt) lacE PTS system lactose‑specific EIICB component
?USA300TCH1516_ALE 2328561 = 118 (1.180)coding (1103/1713 nt) lacE PTS system lactose‑specific EIICB component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 128 total aligned reads displayed.

TTTTCGTATCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAAT  >  USA300TCH1516_ALE/2328334‑2328626
                                                                                                                                                 |                                                                                                                                                   
ttttCGTATCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGt                                                                                                                                                  <  1:463061/149‑1 (MQ=255)
       aTCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACCGCATGCCAAGTAATAGCTAGTACAAATg                                                                                                                                           >  2:280323/1‑149 (MQ=255)
       aTCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTCCGAATg                                                                                                                                           >  1:3953/1‑149 (MQ=255)
       aTCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATg                                                                                                                                           <  1:954558/149‑1 (MQ=255)
        tCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTAATCACGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGa                                                                                                                                          <  1:811888/149‑1 (MQ=255)
         cAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGAc                                                                                                                                         <  1:434328/149‑1 (MQ=255)
           aaGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAGCAATCAAAGTAATAGCTAGTGCGAATGACCa                                                                                                                                       >  1:849713/1‑149 (MQ=255)
           aaGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCa                                                                                                                                       <  2:396001/149‑1 (MQ=255)
            aGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCAt                                                                                                                                      <  2:1050591/149‑1 (MQ=255)
              tttGCTGCAACTTTTCCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATaa                                                                                                                                    <  1:502739/149‑1 (MQ=255)
               ttGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATaaa                                                                                                                                   >  1:551852/1‑149 (MQ=255)
               ttGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATaaa                                                                                                                                   >  1:239979/1‑149 (MQ=255)
               ttGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCGTTACGTCCCTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATaaa                                                                                                                                   <  1:917308/149‑1 (MQ=255)
                    gcAACTTTTTCTTTTAAATCTGAGTTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACGATAAACCaaa                                                                                                                              >  1:828594/1‑149 (MQ=255)
                    gcAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATATTATCTACAACAATCAAAGTAATAGTTAGTACGAATGACATTAAAACaaa                                                                                                                              >  2:469530/1‑149 (MQ=255)
                    gcAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCaaa                                                                                                                              >  1:1014308/1‑149 (MQ=255)
                    gcAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCaaa                                                                                                                              >  1:1050612/1‑149 (MQ=255)
                        cTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCt                                                                                                                          <  2:301153/149‑1 (MQ=255)
                           tttCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTa                                                                                                                       <  2:146900/149‑1 (MQ=255)
                           tttCTTTTAAATCGGAAGTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTa                                                                                                                       <  2:491549/149‑1 (MQ=255)
                           tgtcttctAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTa                                                                                                                       <  2:601768/147‑1 (MQ=255)
                             tCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATCATAATATCTACAACAATCAAAGTAAGAGCTAGTGCGAATGACCATAAACCAAAACCTGTAcc                                                                                                                     <  1:722896/149‑1 (MQ=255)
                             tCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTAcc                                                                                                                     <  1:271063/149‑1 (MQ=255)
                               ttttAAATCTGGATTACTTTCTTTACGTCCTTCTTCTTCATCCAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTAGAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCa                                                                                                                   >  2:210462/1‑149 (MQ=255)
                               ttttAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCa                                                                                                                   >  1:410867/1‑149 (MQ=255)
                                tttAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATGGCTAGTACGAATGACCATAAACCAAAACCTGTACCCAt                                                                                                                  >  1:106935/1‑149 (MQ=255)
                                tttAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAAGAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCAt                                                                                                                  <  2:497371/149‑1 (MQ=255)
                                tgtAAATCTGCATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCAt                                                                                                                  <  2:154110/147‑1 (MQ=255)
                                 ttAAATCTGAATTACTTTCTTTACGTCCTTCTTCTGGATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATg                                                                                                                 <  1:699129/149‑1 (MQ=255)
                                  tAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGa                                                                                                                <  1:1061694/149‑1 (MQ=255)
                                  tAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGa                                                                                                                <  1:657469/149‑1 (MQ=255)
                                   aaaTCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGaa                                                                                                               >  1:795267/1‑148 (MQ=255)
                                   aaaTCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTATACCCATGAt                                                                                                               >  1:972651/1‑149 (MQ=255)
                                    aaTCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATa                                                                                                              >  1:409387/1‑149 (MQ=255)
                                     aTCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATaa                                                                                                             >  1:323879/1‑149 (MQ=255)
                                        tGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGAAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGc                                                                                                          <  1:811974/149‑1 (MQ=255)
                                         taaTTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGcc                                                                                                         <  1:23647/148‑1 (MQ=255)
                                         gAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGcc                                                                                                         >  2:271504/1‑149 (MQ=255)
                                           aTTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCa                                                                                                       >  2:437224/1‑149 (MQ=255)
                                           aTTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCa                                                                                                       >  1:1111835/1‑149 (MQ=255)
                                             tACTTTCTTTACGTCCTTCCTCTTCAACAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAAt                                                                                                     <  1:845535/149‑1 (MQ=255)
                                                tttctttACGTCCTTCTTATTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAATACGAATGACCATAAACCAACAACTGTACCAAGCATAACGCCAACTGGa                                                                                                  >  2:452367/1‑149 (MQ=255)
                                                 ttctttACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGAc                                                                                                 >  2:714561/1‑149 (MQ=255)
                                                      tACGTCTTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGgt                                                                                            <  1:149912/149‑1 (MQ=255)
                                                        cGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGAACATAAACCAATACCTTTACCCATGATAATGCCCAATGGACCTGgtgt                                                                                          >  1:859586/1‑149 (MQ=255)
                                                        cGTCCTTCCTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAGTGGACCTGgtgt                                                                                          >  2:865448/1‑149 (MQ=255)
                                                           ccTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTCAATAATAGTATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGtgttgt                                                                                       >  1:590905/1‑149 (MQ=255)
                                                            cttcttcttCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTc                                                                                      >  1:287389/1‑149 (MQ=255)
                                                             ttcttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTcc                                                                                     <  2:768438/149‑1 (MQ=255)
                                                              tcttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCa                                                                                    >  1:170710/1‑149 (MQ=255)
                                                               tttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTTTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCaa                                                                                   <  1:895626/148‑1 (MQ=255)
                                                               cttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCaa                                                                                   <  1:780936/149‑1 (MQ=255)
                                                               cttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAAGGGACCTGGTGTTGTCCaa                                                                                   <  1:808048/149‑1 (MQ=255)
                                                               cttcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGAGAATGCCCAATGGACCTGGTGTTGTCCaa                                                                                   <  1:677877/149‑1 (MQ=255)
                                                                 tcttcCTCAATAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAgg                                                                                 <  1:363679/149‑1 (MQ=255)
                                                                 tcttcATCAAGAATTTCACTATCATAAACTTTTAGGAGTGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTTAACCAATGATAATACCCAAGGGAACTGGAAGTGTCCAAgg                                                                                 >  2:804316/1‑149 (MQ=255)
                                                                 tcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAgg                                                                                 >  1:74391/1‑149 (MQ=255)
                                                                 tcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAgg                                                                                 >  1:962930/1‑149 (MQ=255)
                                                                 tcttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATACCCAATGGACCTGGTGTTGTCCAAgg                                                                                 >  2:683606/1‑149 (MQ=255)
                                                                  cttcATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGt                                                                                >  1:355987/1‑149 (MQ=255)
                                                                    tcatcaAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTaa                                                                              <  2:272756/149‑1 (MQ=255)
                                                                        caacactttCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACACTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAAGGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTc                                                                          <  2:385511/143‑1 (MQ=255)
                                                                           gAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTcaca                                                                       <  1:107689/149‑1 (MQ=255)
                                                                           gAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTcaca                                                                       <  2:240058/149‑1 (MQ=255)
                                                                           gAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAAGGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTcaca                                                                       <  2:356412/149‑1 (MQ=255)
                                                                                 cACTATCATAAACTTTTAGGAAGGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTaaaa                                                                 >  1:618135/1‑149 (MQ=255)
                                                                                  aCTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAAc                                                                <  1:280323/149‑1 (MQ=255)
                                                                                  aCTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAAc                                                                <  2:3953/149‑1 (MQ=255)
                                                                                          aaaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcatt                                                        >  2:755752/1‑149 (MQ=255)
                                                                                          aaaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcatt                                                        <  1:904272/149‑1 (MQ=255)
                                                                                           aaCTTTTATGAAGGAGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATCGACCTTGTGTTGTCCAAGGTAAATTCACACTAAAACTattcattc                                                       <  2:262406/149‑1 (MQ=255)
                                                                                           aaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcattc                                                       <  1:1007413/149‑1 (MQ=255)
                                                                                            aCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCGTAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAGATTCACACTAAAACTATTCATTcc                                                      >  1:241330/1‑149 (MQ=255)
                                                                                              ttttAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTa                                                    <  1:510569/149‑1 (MQ=255)
                                                                                                       tGGTTAGTAAATAATAATATCTACAACAATCAAAGTAATATCTAGTACGAATAAAAAAAAAACAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCaa                                           <  2:1015649/149‑1 (MQ=255)
                                                                                                            agtaAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGaaa                                      <  1:1035786/149‑1 (MQ=255)
                                                                                                               aaaTAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGt                                   <  1:486758/149‑1 (MQ=255)
                                                                                                               aaaTAATAATATCTACAACAATCAAAGTAATAGCGAGTACGAATGACCATAAACCAAACCCTGTAACCATGATACTGCACCACTGACCTTTCGTTGGCCAAGATAATATCACACTAAAACTATTCCTTCCTAACACTTCAACGAAAAGt                                   <  2:236995/149‑1 (MQ=255)
                                                                                                                     taataTCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCGAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGaaa                             >  2:512730/1‑149 (MQ=255)
                                                                                                                      aataTCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGaaaa                            >  2:529202/1‑149 (MQ=255)
                                                                                                                        tatCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCTCAATGGTCCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATc                          <  2:953452/149‑1 (MQ=255)
                                                                                                                        aaccTACAACAATCAAAGTAAAATCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATACTGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAGCTAATCATTGCTAACACTTCAACGAAAAGTTTGAAAATc                          <  2:518813/146‑1 (MQ=255)
                                                                                                                          tCTACAACAATCAAAGTAATAGATAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCa                        >  1:617177/1‑149 (MQ=255)
                                                                                                                            tACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTATGAAAATCCATa                      >  2:1004041/1‑149 (MQ=255)
                                                                                                                               aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                   <  1:13163/149‑1 (MQ=255)
                                                                                                                               aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                   >  1:356252/1‑149 (MQ=255)
                                                                                                                               aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                   <  1:47952/149‑1 (MQ=255)
                                                                                                                               aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                   <  1:56647/149‑1 (MQ=255)
                                                                                                                                 caaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGAACCTGGTGTAGACCAAGGTAAATTCACACTAAAAATATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATAAGTTa                 >  2:903048/1‑149 (MQ=255)
                                                                                                                                   aTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAAc               >  2:336371/1‑149 (MQ=255)
                                                                                                                                    tCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAACTTCACACTAAAACTATTCATTCCTAACACTTAAACGAAAAGTTTGAAAATCCATACGTTAACa              >  1:754359/1‑149 (MQ=255)
                                                                                                                                      aaaGTGATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGCTAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACGCTTCTACGAAGAGTTTGCAAATCCATAGGTTAACagt            >  1:853867/1‑147 (MQ=255)
                                                                                                                                       aaGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGGAAATTCGCACTAAAACTATTCATTCCCAACACTTCAGAGAAAAGTTTGAAAATCCATAACTTAACACtt           >  2:320551/1‑149 (MQ=255)
                                                                                                                                        aGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTg          >  2:159059/1‑149 (MQ=255)
                                                                                                                                            aTAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAAAAAAAAAAAAAAAAGTTTGAAAATCCATACGTTAACAATTGGTGc      <  2:580232/149‑1 (MQ=255)
                                                                                                                                             tAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGACCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTACCAATTGGTGca     >  2:1023077/1‑148 (MQ=255)
                                                                                                                                              aGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACCCTTCAACGAAAAGATTGAAAAACCATACGTTAACAATTGGTGcaa    >  2:750540/1‑147 (MQ=255)
                                                                                                                                              aGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATCCGTTAACAATTGGTGCTa    >  1:286813/1‑149 (MQ=255)
                                                                                                                                               gCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTaa   >  2:414209/1‑149 (MQ=255)
                                                                                                                                                cTAGTACGAATGACCATAAACCAAAGCCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAAt  <  2:532554/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTTTCGTATCAAAGTTTGCTGCAACTTTTTCTTTTAAATCTGAATTACTTTCTTTACGTCCTTCTTCTTCATCAAGAATTTCACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAAT  >  USA300TCH1516_ALE/2328334‑2328626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.