New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2328479 =128 (1.250)4 (0.040) 3/288 8.8 3.2% coding (1185/1713 nt) lacE PTS system lactose‑specific EIICB component
?USA300TCH1516_ALE 2328561 = 118 (1.180)coding (1103/1713 nt) lacE PTS system lactose‑specific EIICB component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

CACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGAATGTTGGT  >  USA300TCH1516_ALE/2328415‑2328707
                                                                                                                                                  |                                                                                                                                                  
cACTATCATAAACTTTTAGGAAGGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTaaaa                                                                                                                                                  >  1:618135/1‑149 (MQ=255)
 aCTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAAc                                                                                                                                                 <  1:280323/149‑1 (MQ=255)
 aCTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAAc                                                                                                                                                 <  2:3953/149‑1 (MQ=255)
        tAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCGTGATAAAGCCCAATGGACCTGGTATTGTCCAAGGTAAATTAACACTAAAACTattcat                                                                                                                                          >  2:736547/1‑149 (MQ=255)
         aaaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcatt                                                                                                                                         <  1:904272/149‑1 (MQ=255)
         aaaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcatt                                                                                                                                         >  2:755752/1‑149 (MQ=255)
          aaCTTTTATGAAGGAGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATCGACCTTGTGTTGTCCAAGGTAAATTCACACTAAAACTattcattc                                                                                                                                        <  2:262406/149‑1 (MQ=255)
          aaCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcattc                                                                                                                                        <  1:1007413/149‑1 (MQ=255)
          aaCTTTTAGGAATGGGTAGTAAATAATAATAGCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTattcattc                                                                                                                                        <  2:590905/149‑1 (MQ=255)
           aCTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCGTAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAGATTCACACTAAAACTATTCATTcc                                                                                                                                       >  1:241330/1‑149 (MQ=255)
             ttttAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTa                                                                                                                                     <  1:510569/149‑1 (MQ=255)
                           agtaAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGaaa                                                                                                                       <  1:1035786/149‑1 (MQ=255)
                           aaaaaaTAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGaaa                                                                                                                       <  2:103694/146‑1 (MQ=255)
                              aaaTAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGt                                                                                                                    <  1:985658/149‑1 (MQ=255)
                              aaaTAATAATATCTACAACAATCAAAGTAATAGCGAGTACGAATGACCATAAACCAAACCCTGTAACCATGATACTGCACCACTGACCTTTCGTTGGCCAAGATAATATCACACTAAAACTATTCCTTCCTAACACTTCAACGAAAAGt                                                                                                                    <  2:236995/149‑1 (MQ=255)
                                    taataTCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCGAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGaaa                                                                                                              >  2:512730/1‑149 (MQ=255)
                                     aataTCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGaaaa                                                                                                             <  2:287389/149‑1 (MQ=255)
                                       tatCTACAACAATCAAAGTAGTAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAGACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATc                                                                                                           >  1:789319/1‑149 (MQ=255)
                                       tatCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAACACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATc                                                                                                           <  2:345429/149‑1 (MQ=255)
                                       tatCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCTCAATGGTCCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATc                                                                                                           <  2:953452/149‑1 (MQ=255)
                                       aaccTACAACAATCAAAGTAAAATCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATACTGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAGCTAATCATTGCTAACACTTCAACGAAAAGTTTGAAAATc                                                                                                           <  2:518813/146‑1 (MQ=255)
                                         tCTACAACAATCAAAGTAATAGATAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCa                                                                                                         >  1:617177/1‑149 (MQ=255)
                                           tACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTATGAAAATCCATa                                                                                                       >  2:1004041/1‑149 (MQ=255)
                                              aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                                                                                                    <  1:13163/149‑1 (MQ=255)
                                              aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                                                                                                    <  1:47952/149‑1 (MQ=255)
                                              aacaaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                                                                                                    >  1:356252/1‑149 (MQ=255)
                                              aacaaTCAAAGTAATAGCTAGGACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGt                                                                                                    <  2:849713/149‑1 (MQ=255)
                                                caaTCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGAACCTGGTGTAGACCAAGGTAAATTCACACTAAAAATATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATAAGTTa                                                                                                  >  2:903048/1‑149 (MQ=255)
                                                   tCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAACTTCACACTAAAACTATTCATTCCTAACACTTAAACGAAAAGTTTGAAAATCCATACGTTAACa                                                                                               >  1:754359/1‑149 (MQ=255)
                                                   tCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAACGTTTGAAAATCCATACGTTAACa                                                                                               <  2:409387/149‑1 (MQ=255)
                                                     aaaGTGATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGCTAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACGCTTCTACGAAGAGTTTGCAAATCCATAGGTTAACagt                                                                                             >  1:853867/1‑147 (MQ=255)
                                                      aaGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGGAAATTCGCACTAAAACTATTCATTCCCAACACTTCAGAGAAAAGTTTGAAAATCCATAACTTAACACtt                                                                                            >  2:320551/1‑149 (MQ=255)
                                                       aGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTg                                                                                           >  2:159059/1‑149 (MQ=255)
                                                        gTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTgg                                                                                          >  1:1052134/1‑149 (MQ=255)
                                                          aaTAGCTAGTACGAATGACCATACACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTg                                                                                        <  1:210462/149‑1 (MQ=255)
                                                           acaGCTAGTACGAATGACCATAAACCAAAACCTGGACCCATGATAATGCCCAATGGACCTGGGGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGc                                                                                       <  1:741028/147‑1 (MQ=255)
                                                           aTAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGc                                                                                       <  1:123822/149‑1 (MQ=255)
                                                           aTAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAAAAAAAAAAAAAAAAGTTTGAAAATCCATACGTTAACAATTGGTGc                                                                                       <  2:580232/149‑1 (MQ=255)
                                                            tAGCTAGTACGAATGACCATAAACCCAAACAGACACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGca                                                                                      >  2:516229/1‑148 (MQ=255)
                                                            tAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACATTTGGTGCt                                                                                      >  1:82628/1‑149 (MQ=255)
                                                            tAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGACCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTACCAATTGGTGca                                                                                      >  2:1023077/1‑148 (MQ=255)
                                                             aGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACCCTTCAACGAAAAGATTGAAAAACCATACGTTAACAATTGGTGcaa                                                                                     >  2:750540/1‑147 (MQ=255)
                                                             aGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATCCGTTAACAATTGGTGCTa                                                                                     >  1:286813/1‑149 (MQ=255)
                                                             aGCTAGTACGAATGACCATAAACCAAAACCGGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTa                                                                                     <  1:394777/149‑1 (MQ=255)
                                                              gCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTaa                                                                                    >  2:249843/1‑149 (MQ=255)
                                                              gCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTaa                                                                                    <  1:58573/149‑1 (MQ=255)
                                                              gCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTaa                                                                                    >  2:414209/1‑149 (MQ=255)
                                                               cTAGTACGAATGACCATAAACCAAAGCCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAAt                                                                                   <  2:532554/149‑1 (MQ=255)
                                                                 aGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATAc                                                                                 <  1:437224/149‑1 (MQ=255)
                                                                  gTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTACGAATTGGCATTCCTAACACTTCAAAGCAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACa                                                                                <  1:1043384/149‑1 (MQ=255)
                                                                   tACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTCCTAATACaa                                                                               >  2:416384/1‑149 (MQ=255)
                                                                   gaCGAAGGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACaa                                                                               <  1:771508/148‑1 (MQ=255)
                                                                    aCGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACaaa                                                                              <  1:485907/149‑1 (MQ=255)
                                                                      gAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCCACGAAAAGTTTGAAAATCCAGACGTTAACAATTGGTGCTAATACAAATg                                                                            >  1:14559/1‑149 (MQ=255)
                                                                      gAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATg                                                                            >  2:518553/1‑149 (MQ=255)
                                                                       aaTGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATgg                                                                           <  2:867464/149‑1 (MQ=255)
                                                                            gaagaaaCCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATaa                                                                      <  2:828594/145‑1 (MQ=255)
                                                                            ccATAAACCAAACCCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATaa                                                                      <  1:469530/149‑1 (MQ=255)
                                                                            ccATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATaa                                                                      <  2:74391/149‑1 (MQ=255)
                                                                            ccATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGATAATACAAATGGAATaa                                                                      <  2:962930/149‑1 (MQ=255)
                                                                              aTAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGACTAAAg                                                                    >  1:837351/1‑149 (MQ=255)
                                                                              aTAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACAATAAAACTATTCATTCCTAACCCTTCAACGTAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAg                                                                    >  1:983925/1‑149 (MQ=255)
                                                                              aTAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTAGTGGTGTCCAAAGTAAATCCACACTAAAACTATACATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATaagg                                                                    >  1:551729/1‑147 (MQ=255)
                                                                               tAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGa                                                                   <  1:103475/149‑1 (MQ=255)
                                                                               aaaaCCTAAGCCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGa                                                                   <  2:551852/148‑1 (MQ=255)
                                                                               aaaaCCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAACACAACAGGAATAAAGa                                                                   <  2:410916/148‑1 (MQ=255)
                                                                                 aaCCACAACCTGTACCCATGATAATCCCAAATGGCCCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAac                                                                 <  2:1033120/149‑1 (MQ=255)
                                                                                 aaCCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAac                                                                 >  1:488844/1‑149 (MQ=255)
                                                                                         ccttcaCCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGAtt                                                         <  1:276489/144‑1 (MQ=255)
                                                                                          cTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGCATAAAGAACACAGGATTc                                                        >  1:226767/1‑149 (MQ=255)
                                                                                          cTGCACCCACGATAATGCCCAATGCACCTGGTCTTGTCCAAGGTAAACTCACACCAAAACGACTCATTACTAACACTTTAACCATACGTTTGAAAATACATACGTTCGCAAATGGTGCTAATACCACTGGAATAAAGAACACAGGATTc                                                        <  2:241330/149‑1 (MQ=255)
                                                                                           tGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTTGAAATCCATACGTTAGCAACTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCa                                                       <  1:990126/149‑1 (MQ=255)
                                                                                               cccATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATAc                                                   >  2:752960/1‑149 (MQ=255)
                                                                                               cccATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATAc                                                   <  2:489527/149‑1 (MQ=255)
                                                                                               cccATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTACTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATAc                                                   <  2:300758/149‑1 (MQ=255)
                                                                                                ccaaggtAATGCCCAATGGCCCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACATTTAGGGCTAATACAAATGGAATAAAGAACACAGGATTCAATACt                                                  <  1:322230/143‑1 (MQ=255)
                                                                                                 cATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTa                                                 >  1:846563/1‑149 (MQ=255)
                                                                                                   tGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAgt                                               <  2:975580/149‑1 (MQ=255)
                                                                                                   tGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAgt                                               >  1:95284/1‑149 (MQ=255)
                                                                                                   tGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAAGTCACCCTAAAACTAGTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAgt                                               <  1:529202/149‑1 (MQ=255)
                                                                                                    gATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCCTTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAgtg                                              >  1:159068/1‑149 (MQ=255)
                                                                                                        aTGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGc                                          <  1:1065253/149‑1 (MQ=255)
                                                                                                        aTGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGc                                          <  2:413012/149‑1 (MQ=255)
                                                                                                         tGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCa                                         >  1:1034993/1‑149 (MQ=255)
                                                                                                          gCCGAATGGACCTGGTGTTTTCCAAGGTAAATGCACACCAAAACTATTCATTCCGAACACTTCAACGAAAAGTTGGAAAATACACACGTTAACAATTGGTGCTAATAGAAAGGGAATAAAGACCACAGGATTCAATACTAGTGGTGCAc                                        <  2:545044/149‑1 (MQ=255)
                                                                                                           cccAATTGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCAcc                                       <  1:497265/149‑1 (MQ=255)
                                                                                                           cccAATGGATCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCAcc                                       <  1:109929/149‑1 (MQ=255)
                                                                                                            ccAATGGACCTGGTGTTGTCCAAGGTAAGTTCACACCAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCa                                      >  1:723425/1‑149 (MQ=255)
                                                                                                                 ggACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATaa                                 >  2:876757/1‑149 (MQ=255)
                                                                                                                     cTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAGATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACGAGATTCAATACTAGTGGTGCACCAAATAAAAtt                             >  2:625429/1‑149 (MQ=255)
                                                                                                                     cTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAAtt                             >  2:848158/1‑149 (MQ=255)
                                                                                                                        gtgtTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGt                          <  2:452101/149‑1 (MQ=255)
                                                                                                                         tgttgtCCAAGGTAAATTTACACTAAAACTATTCATTCCTAACACTTCATCGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGtt                         <  1:604355/149‑1 (MQ=255)
                                                                                                                            tgtCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGt                      <  1:779673/149‑1 (MQ=255)
                                                                                                                                     tAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACAAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCaa             >  1:206604/1‑149 (MQ=255)
                                                                                                                                        aTTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGa          >  2:33729/1‑149 (MQ=255)
                                                                                                                                        aTTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGa          <  1:271504/149‑1 (MQ=255)
                                                                                                                                            acacTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGAATGt      >  2:935799/1‑149 (MQ=255)
                                                                                                                                            acacTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGAATGt      >  2:392273/1‑149 (MQ=255)
                                                                                                                                                tAAAACTATTCATTCCTAACACTTCAACGAAAAGTATCAAAAGTCAGACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGAATGTTGGt  <  1:755752/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CACTATCATAAACTTTTAGGAATGGGTAGTAAATAATAATATCTACAACAATCAAAGTAATAGCTAGTACGAATGACCATAAACCAAAACCTGTACCCATGATAATGCCCAATGGACCTGGTGTTGTCCAAGGTAAATTCACACTAAAACTATTCATTCCTAACACTTCAACGAAAAGTTTGAAAATCCATACGTTAACAATTGGTGCTAATACAAATGGAATAAAGAACACAGGATTCAATACTAGTGGTGCACCAAATAAAATTGGTTCGTTTACACCAAAGAATGTTGGT  >  USA300TCH1516_ALE/2328415‑2328707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.