New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 277774541 (0.650)3 (0.050) 3/244 NT 6.9% coding (495/705 nt) USA300HOU_2631 transcriptional regulator
?CP000730 = 2777785 42 (0.710)coding (455/705 nt) USA300HOU_2631 transcriptional regulator

AAGTTCATGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCA  >  CP000730/2777613‑2777884
                                                                                                                                    |                                                                                                                                           
aaGTTCATGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAAtt                                                                                                                                      >  2:224268/1‑140 (MQ=255)
   ttCATGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGa                                                                                                                                   <  2:239548/140‑1 (MQ=255)
    tCATGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATTTCAATTTGAt                                                                                                                                  >  2:560996/1‑140 (MQ=255)
       tGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAAt                                                                                                                               <  2:88364/140‑1 (MQ=255)
                 gACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTa                                                                                                                     <  2:388006/140‑1 (MQ=255)
                  aCCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGCTAATTCGATCtttgg                                                                                                                    >  2:163966/1‑138 (MQ=255)
                  aCCAGCTGTTTCCCGGCAACTCCCAGCAATATCACTCACGAGTTGAATAGTTAAAAACTGTTTGATTTCATACAATTAATCTTGACATTATCCACAGGTCTGACGTAGATGGCATATCAATTTGTAAATCCGATCtgtcg                                                                                                                    >  1:406741/1‑136 (MQ=255)
                         gTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAAttt                                                                                                             >  2:58840/1‑140 (MQ=255)
                          tttCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTa                                                                                                            <  1:484550/140‑1 (MQ=255)
                          tttCCCGGGAAATCCCAGCAATAATACTCAGTAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTa                                                                                                            >  2:515188/1‑140 (MQ=255)
                           ttCCCGGGAAATCCCATCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTAc                                                                                                           <  1:40468/140‑1 (MQ=255)
                            tCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACt                                                                                                          >  2:351652/1‑140 (MQ=255)
                                tgtaaaTCCCTGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGtt                                                                                                      <  2:18721/137‑1 (MQ=255)
                                  gAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATtaacaagtaaatttgctaaagatcgaattatcaaattgata                                                                                                    <  1:665579‑M1/140‑42 (MQ=255)
                                  gAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAa                                                                                                    >  1:674707‑M2/1‑139 (MQ=255)
                                     aTCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTAATTTTGGTCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAttgc                                                                                                 <  2:605885‑M2/140‑5 (MQ=255)
                                          aGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGt                                                                                            <  1:575947/140‑1 (MQ=255)
                                          aGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGt                                                                                            <  1:407979/140‑1 (MQ=255)
                                               tatCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAg                                                                                       <  1:515624/140‑1 (MQ=255)
                                                 tCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATtaaaaagtaaatttgctaaagatcgaattatcaaattgatatgccatctatgtcag                                                                                     >  2:596489‑M1/1‑84 (MQ=255)
                                                 tCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTTTTAatgccatctatgtcag                                                                                     <  1:596489‑M2/140‑17 (MQ=255)
                                                       aTGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTTATAATTCTATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCAtgtg                                                                               <  2:51157/140‑1 (MQ=255)
                                                        tGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCCACTGTCTGACATCGACCGCATATCAATTGGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATgtgt                                                                              <  2:181126/140‑1 (MQ=255)
                                                              gAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTtgctgc                                                                        >  1:475879/1‑140 (MQ=255)
                                                              gAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTtgctgc                                                                        <  1:553930/140‑1 (MQ=255)
                                                                atcgTTAAAAACTGTTTGATTTCATAAAATGCATCTTGATCGTATCCTACTGTCTGACATAGGTGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTc                                                                      >  2:425020/4‑140 (MQ=255)
                                                                aTAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTc                                                                      >  1:615579/1‑140 (MQ=255)
                                                                  aGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCAt                                                                    <  1:228567/140‑1 (MQ=255)
                                                                     tAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTAt                                                                 >  2:386159/1‑140 (MQ=255)
                                                                       aaaaCTGTTTGATTTCATAAAATTCTTCTTGATCGTATCCTACTGTCTGACATAGATTGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCa                                                               <  2:141207/140‑1 (MQ=255)
                                                                         aaCTGTTTGATTTCATAAAATTCATCTTGATCGTATTCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCTATCTTTAGCAAATTTACTTGTTATTGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCAtt                                                             <  2:3773/140‑1 (MQ=255)
                                                                               ttGATTTCATAAAATTCATCTTGATCGTATCCTTCTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTaa                                                       <  1:243361/140‑1 (MQ=255)
                                                                                       aTAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCaaaaa                                               <  2:560392/140‑1 (MQ=255)
                                                                                        tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                              <  1:224268/140‑1 (MQ=255)
                                                                                        tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                              <  1:409933/140‑1 (MQ=255)
                                                                                        tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                              <  1:288706/140‑1 (MQ=255)
                                                                                          aaaTTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATCTCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTTTTGCTGCTAATTATCATTTATTAATGCAAAAAgtg                                            >  1:209784/1‑140 (MQ=255)
                                                                                          aaaTTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATGTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAgtt                                            >  2:67901/1‑139 (MQ=255)
                                                                                                     gcttgTATCCTACTGTCTTATATTGATGGCATATCAATTTGATAATTCGATCTTTAGCAAGTTTCCTTGTTAATGCCATGTTATAGTTCATTTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAatata                                 <  1:581313/136‑1 (MQ=255)
                                                                                                     gATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAatata                                 >  2:343573/1‑140 (MQ=255)
                                                                                                          tATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATatcatc                            <  1:214905/140‑1 (MQ=255)
                                                                                                                     cTGACATAGATGGCATtaaaaagtaaatttgctaaagatcgaattatcaaattgatatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtga                 <  1:596489‑M1/140‑125 (MQ=255)
                                                                                                                     cTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTTTTAatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtga                 >  2:596489‑M2/1‑56 (MQ=255)
                                                                                                                      tGACATAGTTCGCATATCAATTTGATAATTCGATATCTACCAAAGTGACTTGTGCCTGGCATGGTATCGTTCATGTGTTGCTGCTAATTATACTTTATTAATGCAAAAACAGACAAACATGAATAATCATTAGCGTGGca                >  2:49068/1‑140 (MQ=255)
                                                                                                                      tGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATCCATGAAAGAAGTGTCAAACATATATCATCATTATCTTTGcg                >  1:676587/1‑139 (MQ=255)
                                                                                                                         cATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACaa             >  1:126774/1‑140 (MQ=255)
                                                                                                                         cATAGATGGCATATCAATTTGATAATTCGATCTCTAGAAAAGTGACTTGGTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACaa             <  1:677308/140‑1 (MQ=255)
                                                                                                                             gATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAg         >  1:355552/1‑140 (MQ=255)
                                                                                                                                ggCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGcc      >  2:18866/1‑140 (MQ=255)
                                                                                                                                ggCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGcc      >  2:379179/1‑140 (MQ=255)
                                                                                                                                 gCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCa     <  1:75937/140‑1 (MQ=255)
                                                                                                                                 gCATATCAATTTGATAATTCCATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCa     <  2:71858/140‑1 (MQ=255)
                                                                                                                                 gCAAtaacaagtaaatttgctaaagatcgaattatcaaattgatatgccatctatgtcagacagtaggatacgaccaaaattaattttatgaaatcaaacagtttttaactattcaactcatgagtgatatggctgggat     <  2:605885‑M1/140‑137 (MQ=255)
                                                                                                                                    ttaacaagtaaatttgctaaagatcgaattatcaaattgatatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtgatatggctgggatttc  >  1:674707‑M1/1‑1 (MQ=255)
                                                                                                                                    tatCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtgatatggctgggatttc  <  1:665579‑M2/140‑100 (MQ=255)
                                                                                                                                    |                                                                                                                                           
AAGTTCATGAATAATATGACCAGCTGTTTCCCGGGAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCA  >  CP000730/2777613‑2777884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.