New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 277774541 (0.650)3 (0.050) 3/244 NT 6.9% coding (495/705 nt) USA300HOU_2631 transcriptional regulator
?CP000730 = 2777785 42 (0.710)coding (455/705 nt) USA300HOU_2631 transcriptional regulator

GAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAAGAACTGTACAATCGGTTAATGC  >  CP000730/2777647‑2777924
                                                                                                                                          |                                                                                                                                           
gAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAa                                                                                                                                            >  1:674707‑M2/1‑139 (MQ=255)
   aTCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTAATTTTGGTCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAttgc                                                                                                                                         <  2:605885‑M2/140‑5 (MQ=255)
        aGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGt                                                                                                                                    <  1:407979/140‑1 (MQ=255)
        aGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGt                                                                                                                                    <  1:575947/140‑1 (MQ=255)
             tatCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAg                                                                                                                               <  1:515624/140‑1 (MQ=255)
               tCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTTTTAatgccatctatgtcag                                                                                                                             <  1:596489‑M2/140‑17 (MQ=255)
                     aTGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTTATAATTCTATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCAtgtg                                                                                                                       <  2:51157/140‑1 (MQ=255)
                      tGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCCACTGTCTGACATCGACCGCATATCAATTGGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATgtgt                                                                                                                      <  2:181126/140‑1 (MQ=255)
                            gAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTtgctgc                                                                                                                <  1:553930/140‑1 (MQ=255)
                            gAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTtgctgc                                                                                                                >  1:475879/1‑140 (MQ=255)
                              atcgTTAAAAACTGTTTGATTTCATAAAATGCATCTTGATCGTATCCTACTGTCTGACATAGGTGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTc                                                                                                              >  2:425020/4‑140 (MQ=255)
                              aTAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTc                                                                                                              >  1:615579/1‑140 (MQ=255)
                                aGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCAt                                                                                                            <  1:228567/140‑1 (MQ=255)
                                   tAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTAt                                                                                                         >  2:386159/1‑140 (MQ=255)
                                     aaaaCTGTTTGATTTCATAAAATTCTTCTTGATCGTATCCTACTGTCTGACATAGATTGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCa                                                                                                       <  2:141207/140‑1 (MQ=255)
                                       aaCTGTTTGATTTCATAAAATTCATCTTGATCGTATTCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCTATCTTTAGCAAATTTACTTGTTATTGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCAtt                                                                                                     <  2:3773/140‑1 (MQ=255)
                                             ttGATTTCATAAAATTCATCTTGATCGTATCCTTCTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTaa                                                                                               <  1:243361/140‑1 (MQ=255)
                                                     aTAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCaaaaa                                                                                       <  2:560392/140‑1 (MQ=255)
                                                      tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                                                                      <  1:288706/140‑1 (MQ=255)
                                                      tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                                                                      <  1:409933/140‑1 (MQ=255)
                                                      tAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAg                                                                                      <  1:224268/140‑1 (MQ=255)
                                                        aaaTTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATCTCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTTTTGCTGCTAATTATCATTTATTAATGCAAAAAgtg                                                                                    >  1:209784/1‑140 (MQ=255)
                                                        aaaTTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATGTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAgtt                                                                                    >  2:67901/1‑139 (MQ=255)
                                                                   gcttgTATCCTACTGTCTTATATTGATGGCATATCAATTTGATAATTCGATCTTTAGCAAGTTTCCTTGTTAATGCCATGTTATAGTTCATTTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAatata                                                                         <  1:581313/136‑1 (MQ=255)
                                                                   gATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAatata                                                                         >  2:343573/1‑140 (MQ=255)
                                                                        tATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATatcatc                                                                    <  1:214905/140‑1 (MQ=255)
                                                                                   cTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTTTTAatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtga                                                         >  2:596489‑M2/1‑56 (MQ=255)
                                                                                    tGACATAGTTCGCATATCAATTTGATAATTCGATATCTACCAAAGTGACTTGTGCCTGGCATGGTATCGTTCATGTGTTGCTGCTAATTATACTTTATTAATGCAAAAACAGACAAACATGAATAATCATTAGCGTGGca                                                        >  2:49068/1‑140 (MQ=255)
                                                                                    tGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATCCATGAAAGAAGTGTCAAACATATATCATCATTATCTTTGcg                                                        >  1:676587/1‑139 (MQ=255)
                                                                                       cATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACaa                                                     >  1:126774/1‑140 (MQ=255)
                                                                                       cATAGATGGCATATCAATTTGATAATTCGATCTCTAGAAAAGTGACTTGGTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACaa                                                     <  1:677308/140‑1 (MQ=255)
                                                                                           gATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAg                                                 >  1:355552/1‑140 (MQ=255)
                                                                                              ggCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGcc                                              >  2:18866/1‑140 (MQ=255)
                                                                                              ggCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGcc                                              >  2:379179/1‑140 (MQ=255)
                                                                                               gCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCa                                             <  1:75937/140‑1 (MQ=255)
                                                                                               gCATATCAATTTGATAATTCCATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCa                                             <  2:71858/140‑1 (MQ=255)
                                                                                                  tatCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAatgccatctatgtcagacagtaggatacgatcaagatgaattttatgaaatcaaacagtttttaactattcaactcatgagtgatatggctgggatttc                                          <  1:665579‑M2/140‑100 (MQ=255)
                                                                                                   atCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCaa                                         <  2:475879/140‑1 (MQ=255)
                                                                                                       aTTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATtct                                     <  1:322214/140‑1 (MQ=255)
                                                                                                             tAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGc                               >  1:426651/1‑140 (MQ=255)
                                                                                                                 tCGAACTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTCCAGGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATc                           <  1:393227/140‑1 (MQ=255)
                                                                                                                    ttttttAGTAAATTTTCTTGTTAATGCCAGTTTATAGTTAATGTGTTTCCGCTCATTATCATTTATTAATGCAAACATTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCCTCCATTCTTTAGGCAATCCaa                        <  2:642670/137‑1 (MQ=255)
                                                                                                                      cTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAATGCCATCAATTCTCTAGGCAATCCAAGa                      >  1:702854/1‑140 (MQ=255)
                                                                                                                         tAGCAAATTTACTTGTTAATGCCATGTTCTAGTTCATTTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATAAATTCTCTAGGCACTCCAGGAACt                   >  1:208047/1‑140 (MQ=255)
                                                                                                                         tAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCATTGCACAAAAAGGCCATCAATTCCCTAGGCAATCCAAGAAca                   >  1:45904/1‑139 (MQ=255)
                                                                                                                                 ttACTTGTTAATGCCATGTTATAGTTTATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAAGAACTGTACAATc           <  1:296160/140‑1 (MQ=255)
                                                                                                                                  tACTAGTTAATGCCATGTTATAGTTCATGTGTCGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAACAACTGTACAATCg          >  1:520272/1‑140 (MQ=255)
                                                                                                                                     ttGTTAATGCCATGTTATAGTTCATGTTGTGCTGGTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAAGAACTGTACAATCGGtt       <  2:308152/140‑1 (MQ=255)
                                                                                                                                     ttGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAAGAACTGTACAATCGGtt       <  1:78285/140‑1 (MQ=255)
                                                                                                                                          aaTGCTATTGTATAGTTCATGTGTTGCTGCGCTTTATCCTATATTAATGCAAAAAGTGTGAAAAATATATCATCATTAGCTTTGCACACAAAGGTCATCAATTCTCTAGGCAATCCAAGAACTGTACAATCGGTTAATGc  <  2:236338/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GAAATCCCAGCCATATCACTCATGAGTTGAATAGTTAAAAACTGTTTGATTTCATAAAATTCATCTTGATCGTATCCTACTGTCTGACATAGATGGCATATCAATTTGATAATTCGATCTTTAGCAAATTTACTTGTTAATGCCATGTTATAGTTCATGTGTTGCTGCTCATTATCATTTATTAATGCAAAAAGTGTCAAAAATATATCATCATTAGCTTTGCACAAAAAGGCCATCAATTCTCTAGGCAATCCAAGAACTGTACAATCGGTTAATGC  >  CP000730/2777647‑2777924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.