New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 267142045 (0.920)3 (0.060) 3/250 NT 6.5% coding (171/345 nt) USA300HOU_2526 possible thioredoxin
?CP000730 = 2671462 43 (0.920)coding (129/345 nt) USA300HOU_2526 possible thioredoxin

TCATTTGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACGGATATTGATGAATAAATTCACGGA  >  CP000730/2671281‑2671559
                                                                                                                                            |                                                                                                                                           
tcattTGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACttt                                                                                                                                            <  2:568637/141‑1 (MQ=255)
tcagtTGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACttt                                                                                                                                            <  1:410537/141‑1 (MQ=255)
   tttGCTTAAGATGATGTTCAAATCGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGAt                                                                                                                                         <  2:532340/141‑1 (MQ=255)
   tttGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGAt                                                                                                                                         <  1:351293/141‑1 (MQ=255)
   tttGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTCTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGAt                                                                                                                                         <  1:384727/141‑1 (MQ=255)
   tttGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCATTGACGATGCATTTATTTACCATACATAAAAATTAAATCAACAGGTACAGTGAAAATATGTAATTCTCCAGCAATAGCTTCCACCTGACTTTGAt                                                                                                                                         >  2:392967/1‑141 (MQ=255)
       cTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAAt                                                                                                                                     <  2:504359/141‑1 (MQ=255)
        ttAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATc                                                                                                                                    <  1:121092/141‑1 (MQ=255)
                    tCACAACGTTGCATCTCGATAAAACGACCTTGACGATGCATTCCATTTCCATTCATAAAAACTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGc                                                                                                                        <  2:82175/141‑1 (MQ=255)
                    tCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTAAATTTGATTAATCACAGCTAATGGc                                                                                                                        >  1:3231/1‑141 (MQ=255)
                     cAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGca                                                                                                                       >  2:365660/1‑141 (MQ=255)
                        aaCGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACAt                                                                                                                    <  2:228775/141‑1 (MQ=255)
                        aaCGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACAt                                                                                                                    >  1:124061/1‑141 (MQ=255)
                           gTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGACCATTgg                                                                                                                 >  1:334940/1‑141 (MQ=255)
                              gCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGat                                                                                                              >  1:522303/1‑141 (MQ=255)
                               cATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGatt                                                                                                             >  2:66151/1‑140 (MQ=255)
                               cATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGata                                                                                                             <  1:541004/141‑1 (MQ=255)
                                atatCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGatat                                                                                                            <  2:10967/141‑1 (MQ=255)
                                   tCGATAAAACGCCCTTGACGATGCTTTTTCTTTCCATTCATAAAAATTAAATCCACTGTTACATTGAAAATATTTAATTCTCCAGCAATAGCTACCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGAt                                                                                                         <  2:372166/141‑1 (MQ=255)
                                     gATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTg                                                                                                       <  2:409053/141‑1 (MQ=255)
                                      acaaatCGCCCTGGCAGATGTATTTCATTTCCATTCATAGAAAGTAAATCCATAGGTACAGGGAAAATATGTAATTCTCCAGCAATAGCTTCCACCTGACGTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGc                                                                                                      <  2:534005/139‑1 (MQ=255)
                                      aTAAAACGCCCTTGACGATGCATTTCTTTTCCTGTCATAACAATTAAATCCACAGTTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTgatgcaatcacatcccaatctgccattagctgtgattaa                                                                                                      <  2:118839‑M1/141‑40 (MQ=255)
                                         aaaCGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACCTTGGGATATGATTGCATc                                                                                                   >  1:539431/1‑141 (MQ=255)
                                           aCGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCATCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTACATCaa                                                                                                 <  2:264638‑M2/141‑3 (MQ=255)
                                           aCGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCaa                                                                                                 <  2:475811‑M2/141‑3 (MQ=255)
                                              cccTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCaagtc                                                                                              >  1:153276‑M2/1‑136 (MQ=255)
                                              cccTTGACGAAGCATTTCATTTCCATTCATAAAAATTAAATCCACAGGGACAGTGAAAATATTTAATTCTCCAGCAATAGTTTCCACCTGACTTcatgcaatcatatcccaatgtgccattagctgtgattaatcaaagtc                                                                                              <  2:27293‑M1/141‑48 (MQ=255)
                                               ccTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCTTCaggact                                                                                             <  1:337664‑M2/141‑7 (MQ=255)
                                               ccTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCt                                                                                             >  2:263639/1‑141 (MQ=255)
                                               ccTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCt                                                                                             <  2:248055/141‑1 (MQ=255)
                                                 ttGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGAATATGATTGCATCAAGTCTTc                                                                                           >  2:411881/1‑141 (MQ=255)
                                                   gACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCaa                                                                                         >  2:84568/1‑141 (MQ=255)
                                                      gATTCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAAttt                                                                                      <  2:412857/141‑1 (MQ=255)
                                                        tGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTtgt                                                                                    >  1:225793/1‑141 (MQ=255)
                                                                  ttCCATTCATAAATATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTTATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACgg                                                                          <  2:251715/141‑1 (MQ=255)
                                                                  ttCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGCCTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGCCTTCAATCTGTAGTAAAACga                                                                          >  1:76157/1‑140 (MQ=255)
                                                                  ttCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACgg                                                                          <  2:178335/141‑1 (MQ=255)
                                                                  ttCCATTCATAAAAAATAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACgg                                                                          <  2:247769/141‑1 (MQ=255)
                                                                    ccATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCa                                                                        >  1:309834/1‑141 (MQ=255)
                                                                    ccATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCa                                                                        <  1:196446/141‑1 (MQ=255)
                                                                     cATTCACAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATTGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCAt                                                                       <  2:36508/141‑1 (MQ=255)
                                                                          aTAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGacac                                                                  <  2:250293/141‑1 (MQ=255)
                                                                              aaatcaaatcCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGCATATGCTTGCATCCAGCCTTCAGTTTGTGGTAAAACGGCATGACACACGc                                                              >  2:100989/1‑141 (MQ=255)
                                                                              aaaTTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGc                                                              <  1:84568/141‑1 (MQ=255)
                                                                               aaTTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCt                                                             <  1:406234/141‑1 (MQ=255)
                                                                               aaTTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCt                                                             <  1:324494/141‑1 (MQ=255)
                                                                               aaTTAAATCCACAGGTACAGCGCCAATATTTCATTCTACCGCAATCCCTCCCACCTGACTGTTAGTAATCACAGCGTATTGCACATTCGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCt                                                             <  2:49275/141‑1 (MQ=255)
                                                                                aTTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCAAATAGGTATGTTATTGCATCAAGTCTTCAATTTGGGGTAAAACGGCATGACACACGCTa                                                            >  1:159003/1‑141 (MQ=255)
                                                                                        cacaGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCaaagtcaggtggaagctattgctggagaattaaatattttcactgta                                                    >  2:528911‑M2/1‑94 (MQ=255)
                                                                                             gTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCa                                               >  1:546311/1‑141 (MQ=255)
                                                                                              tACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTgatgcaatcatatcccaatgtgccattagctgtgattaatcaaagtcaggtggaagctattgctggagaattaaatattttcactgtacctgtg                                               >  2:528911‑M1/1‑47 (MQ=255)
                                                                                                cAGTGAAAAGATTTAATTCTCCAGCACGATCTTCCACCTGACTTTGATTAAACACAGCTCATGGCACATTGGTATAAGATAGCATCTACCCTTCAATATGGGGTAAAACAACAGGACACAAGCAACACTGATGCCGCAGGa                                            >  2:10162/1‑141 (MQ=255)
                                                                                                      aaaTATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACAGTGATCGCGCATGACATGTa                                      >  1:131138/1‑141 (MQ=255)
                                                                                                      aaaTATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATAAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTTTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATgga                                      >  1:430701/1‑139 (MQ=255)
                                                                                                              aaTTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATGGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATAACACACCCTACACAGATCGCGAATGCAGCGTACAACTGcc                              >  1:530422/1‑140 (MQ=255)
                                                                                                                 tctcCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAAc                           <  1:365660/141‑1 (MQ=255)
                                                                                                                  ctcCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACg                          <  1:521096/141‑1 (MQ=255)
                                                                                                                          aTAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACGGATATtga                  <  1:47686/141‑1 (MQ=255)
                                                                                                                               ttCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACGGATATTGATGAAt             <  2:522303/141‑1 (MQ=255)
                                                                                                                                      gaagacttGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACGGATATTGATGAATAACTTc       >  2:367334/7‑141 (MQ=255)
                                                                                                                                       gACTTgatgcaatcatatcccaatgtgccattagctgtgattaatcaaagtcaggtggaagctattgctggagaattaaatattttcactgtacctgtggatttaatttttatgaatggaaaagaaatgcatcgtcaaggg     >  1:153276‑M1/1‑5 (MQ=255)
                                                                                                                                       gACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATGaagtcaggtggaaactattgctggagaattaaatattttcactgtccctgtggatttaatttttatgaatggaaatgaaatgcttcgtcaaggg     <  2:27293‑M2/141‑95 (MQ=255)
                                                                                                                                       agtcctgaagcaatcatatcccaatgtgccattagctgtgattaatcaaagtcaggtggaagctattgctggagaattaaatattttcactgtacctgtggatttaatttttatgaatggaaaagaaatgcatcgtcaagg      <  1:337664‑M1/136‑136 (MQ=255)
                                                                                                                                           ttgatgtaatcatatcccaatgtgccattagctgtgattaatcaaagtcaggtggaagctattgatggagaattaaatattttcactgtacctgtggatttaatttttatgaatggaaaagaaatgcatcgtcaagggcgt  <  2:264638‑M1/141‑140 (MQ=255)
                                                                                                                                           ttgatgcaatcatatcccaatgtgccattagctgtgattaatcaaagtcaggtggaagctattgctggagaattaaatattttcactgtacctgtggatttaatttttatgaatggaaaagaaatgcatcgtcaagggcgt  <  2:475811‑M1/141‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCATTTGCTTAAGATGATGTTCAAAACGTTGCATATCGATAAAACGCCCTTGACGATGCATTTCTTTTCCATTCATAAAAATTAAATCCACAGGTACAGTGAAAATATTTAATTCTCCAGCAATAGCTTCCACCTGACTTTGATTAATCACAGCTAATGGCACATTGGGATATGATTGCATCAAGTCTTCAATTTGTGGTAAAACGGCATGACACACGCTACACTGATCGCGCATGACATGTACAACTGCTAACGGATATTGATGAATAAATTCACGGA  >  CP000730/2671281‑2671559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.